GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Glycogene class
A3GALT2
A4GALT
A4GNT
ABO
ALG1
ALG10
ALG10B
ALG11
ALG12
ALG13
ALG14
ALG1L
ALG1L2
ALG2
ALG3
ALG5
ALG6
ALG8
ALG9
B3GALNT1
B3GALNT2
B3GALT1
B3GALT2
B3GALT4
B3GALT5
B3GAT1
B3GAT2
B3GNT2
B3GNT3
B3GNT4
B3GNT5
B3GNT6
B3GNT7
B3GNT8
B4GALNT1
B4GALNT2
B4GALNT3
B4GALNT4
B4GALT1
B4GALT2
B4GALT3
B4GALT4
B4GALT5
B4GALT6
C1GALT1
C1GALT1C1
CHST1
CHST10
CHST2
CHST3
CHST4
CHST5
CHST8
CHST9
CMAS
CTBS
DAD1
DDOST
DPAGT1
DPM1
DPM2
DPM3
EDEM1
EDEM2
EDEM3
ENGASE
FPGT
FUCA1
FUT1
FUT10
FUT11
FUT2
FUT3
FUT4
FUT5
FUT6
FUT7
FUT8
FUT9
GAL3ST1
GAL3ST2
GAL3ST3
GAL3ST4
GALNT1
GALNT10
GALNT11
GALNT12
GALNT13
GALNT14
GALNT15
GALNT16
GALNT17
GALNT18
GALNT2
GALNT3
GALNT4
GALNT5
GALNT6
GALNT7
GALNT8
GALNT9
GALNTL6
GANAB
GBGT1
GCNT1
GCNT2
GCNT3
GCNT4
GNPNAT1
GNPTAB
GNPTG
HEXA
HEXB
MAN1A1
MAN1A2
MAN1B1
MAN1C1
MAN2A1
MAN2A2
MAN2B1
MAN2B2
MAN2C1
MANBA
MANEA
MGAT1
MGAT2
MGAT3
MGAT4A
MGAT4B
MGAT4C
MGAT4D
MGAT5
MOGS
NAGA
NAGK
NAGPA
NEU1
NEU2
NEU3
NEU4
PRKCSH
RPN1
RPN2
ST3GAL1
ST3GAL2
ST3GAL3
ST3GAL4
ST3GAL5
ST3GAL6
ST6GAL1
ST6GAL2
ST6GALNAC1
ST6GALNAC2
ST6GALNAC3
ST6GALNAC4
ST6GALNAC5
ST6GALNAC6
ST8SIA1
ST8SIA2
ST8SIA3
ST8SIA4
ST8SIA5
ST8SIA6
STT3A
STT3B
UGCG
UGGT1
UGGT2
UGT8
Pathway Map
ST8SIA3



General Information
Gene Symbol:   
ST8SIA3
Protein name:   
Sia-alpha-2,3-Gal-beta-1,4-GlcNAc-R:alpha 2,8-sialyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT29
E.C. No:   
2.4.99.8
Organs:   
Reaction (SNFG):   
+
→
+ CMP
Reaction (IUBMB):   
No specified reaction.
About

• ST8Sia-III transfers sialic acids to Siaα2-3Galβl-4GlcNAc sequences on N-linked oligosaccharides of glycoproteins or α2-3sialylated glycosphingolipids.
• The substrate specificity of ST8Sia-III toward glycolipids is similar to that of ST8Sia-I (GD3 synthase), and both enzymes synthesize GD3 from GM3.
• However, ST8Sia-III (and perhaps ST8Sia-5), but not ST8Sia-I, is thought to synthesize GT3 from GD3.
• The mouse ST8Sia-III gene (st8sia3) was expressed only in the brain and testis.
• Broad specificity for GSLs and proteins

Related Pathway
KEGG ORTHOLOGY:   
K06613
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-4085001
Related Database
Organism:   
Homo sapiens
Uniprot:   
O43173 
NCBI GeneID:   
51046
GlyMap GeneID:   
51046
DNA aRefSeq:   
NM_015879.2 
Protein RefSeq:   
NP_056963.2 
BRENDA:   
Kinetic info.
OMIM number:   
609478
GeneCards database:   
ST8SIA3
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GSL
Compartment:  
     
Substrate:  
     NeuAc(a2-3)
Product:  
     NeuAc(a2-8)NeuAc(a2-3)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 8 6 4 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000324000 MYCN RUNX1 MAX
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-889-5p 1358-1365 -0.369 98.0 -0.369 98.0 noncon
hsa-miR-4260 41-48 -0.357 96.0 -0.357 97.0 noncon
hsa-miR-601 7437-7444 -0.355 95.0 -0.355 96.0 noncon
hsa-miR-212-5p 2246-2253 -0.321 96.0 -0.321 97.0 noncon
hsa-miR-6760-5p 463-470 -0.307 98.0 -0.307 98.0 noncon
hsa-miR-509-3p 5416-5423 -0.306 93.0 -0.306 95.0 noncon
hsa-miR-642a-3p 8133-8140 -0.303 97.0 -0.303 98.0 noncon
hsa-miR-642b-3p 8133-8140 -0.303 97.0 -0.303 98.0 noncon
hsa-miR-1825 1200-1207 -0.3 97.0 -0.3 98.0 noncon
hsa-miR-6752-3p 7322-7329 -0.298 94.0 -0.298 96.0 noncon
hsa-miR-6505-3p 7934-7941 -0.297 95.0 -0.297 96.0 noncon
hsa-miR-6804-3p 7095-7102 -0.296 94.0 -0.296 96.0 noncon
hsa-miR-3184-5p 6244-6251 -0.291 92.0 -0.291 94.0 noncon
hsa-miR-6841-5p 1030-1037 -0.29 93.0 -0.29 95.0 noncon
hsa-miR-423-5p 6244-6251 -0.283 91.0 -0.283 93.0 noncon
hsa-miR-3162-5p 464-470 -0.282 92.0 -0.282 95.0 noncon
hsa-miR-4515 708-715 -0.28 93.0 -0.28 94.0 noncon
hsa-miR-6871-5p 899-906 -0.277 95.0 -0.277 96.0 noncon
hsa-miR-5588-3p 231-238 -0.274 95.0 -0.274 96.0 noncon
hsa-miR-8071 3824-3831 -0.274 94.0 -0.274 96.0 noncon
hsa-miR-346 7339-7346 -0.27 90.0 -0.27 93.0 noncon
hsa-miR-33b-3p 3407-3414 -0.27 94.0 -0.27 96.0 noncon
hsa-miR-563 5779-5786 -0.268 92.0 -0.268 95.0 noncon
hsa-miR-4732-3p 8137-8144 -0.266 97.0 -0.266 98.0 noncon
hsa-miR-519e-3p 3407-3414 -0.259 93.0 -0.259 95.0 noncon
hsa-miR-6838-5p 239-246 -0.253 92.0 -0.253 94.0 con