GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Glycogene class
A3GALT2
A4GALT
A4GNT
ABO
B3GALNT1
B3GALNT2
B3GALT1
B3GALT2
B3GALT4
B3GALT5
B3GAT1
B3GAT2
B3GNT2
B3GNT3
B3GNT4
B3GNT5
B3GNT6
B3GNT7
B4GALNT1
B4GALNT2
B4GALNT3
B4GALNT4
B4GALT1
B4GALT2
B4GALT3
B4GALT4
B4GALT5
B4GALT6
C1GALT1
C1GALT1C1
CHST1
CHST10
CHST2
CHST3
CHST4
CHST5
CHST8
CHST9
CMAS
FPGT
FUT1
FUT2
FUT3
FUT4
FUT5
FUT6
FUT7
FUT9
GAL3ST1
GAL3ST2
GAL3ST3
GAL3ST4
GALNT1
GALNT10
GALNT11
GALNT12
GALNT13
GALNT14
GALNT15
GALNT16
GALNT17
GALNT18
GALNT2
GALNT3
GALNT4
GALNT5
GALNT6
GALNT7
GALNT8
GALNT9
GALNTL6
GBGT1
GCNT1
GCNT2
GCNT3
GCNT4
HEXA
HEXB
NAGA
NEU1
NEU2
NEU3
NEU4
ST3GAL1
ST3GAL2
ST3GAL3
ST3GAL4
ST3GAL5
ST3GAL6
ST6GAL1
ST6GAL2
ST6GALNAC1
ST6GALNAC2
ST6GALNAC3
ST6GALNAC4
ST6GALNAC5
ST6GALNAC6
ST8SIA1
ST8SIA2
ST8SIA3
ST8SIA4
ST8SIA5
ST8SIA6
UGCG
UGT8
Pathway Map
GAL3ST4



General Information
Gene Symbol:   
GAL3ST4
Protein name:   
Galactose-3-O-sulfotransferase 4
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.*
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• There are 4 enzymes that perform 3-O-sulfation of Gal.
• Gal3ST1: Acts on GalCer and LacCer to create sulfated glycolipids. Catalyzes the synthesis of sulfatides and seminolpids.
• Gal3ST2: Galβ1,4GlcNAc of O- and N-glycan, can act on sulfated substrates to form bisulfated carbohydrates like 3′,6-disulfo LewisX and 3′,6'-disulfo LewisX. This can also form 3'-sulfo-Le a and 3-O-sulfo-LeX determinants and it can act on lipid substrates
• Gal3ST3 Galβ1,4GlcNAc of O- and N-glycan and Galβ1,4GalNAc of GloboH structure. Can act on sulfated substrates to form bisulfated carbohydrates
• Gal3ST4 Galβ1,4GalNAc of Globo glycolipids and glycopeptides

Related Pathway
KEGG ORTHOLOGY:   
K09677
KEGG Pathway:   
No Record 
Reactome:   
No Record
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q96RP7 
NCBI GeneID:   
79690
GlyMap GeneID:   
79690
DNA aRefSeq:   
NM_024637.4 
Protein RefSeq:   
NP_078913.3 
BRENDA:   
Kinetic info.
OMIM number:   
608235
GeneCards database:   
GAL3ST4
Enzyme Specificity
Glycan Type:  
     O-linked (mucin type), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-3)GalNAc(b1-?)
Product:  
     Gal(b1-3 [3S])GalNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6795-5p 680-687 -0.862 99.0 -0.862 99.0 noncon
hsa-miR-6887-5p 680-687 -0.838 99.0 -0.838 99.0 noncon
hsa-miR-7109-5p 681-688 -0.712 99.0 -0.712 99.0 noncon
hsa-miR-3651 338-345 -0.554 99.0 -0.554 99.0 noncon
hsa-miR-598-5p 1017-1024 -0.542 98.0 -0.445 98.0 noncon
hsa-miR-4285 226-233 -0.531 97.0 -0.531 98.0 noncon
hsa-miR-3184-5p 461-468 -0.519 99.0 -0.519 99.0 con
hsa-miR-325 1118-1125 -0.505 99.0 -0.416 98.0 noncon
hsa-miR-423-5p 461-468 -0.503 99.0 -0.503 99.0 con
hsa-miR-6886-3p 535-542 -0.498 99.0 -0.498 99.0 noncon
hsa-miR-4537 752-759 -0.491 97.0 -0.491 98.0 noncon
hsa-miR-339-5p 868-875 -0.484 98.0 -0.399 98.0 noncon
hsa-miR-1307-3p 228-235 -0.467 99.0 -0.467 99.0 noncon
hsa-miR-6848-5p 363-370 -0.467 97.0 -0.467 97.0 noncon
hsa-miR-6846-5p 363-370 -0.459 96.0 -0.459 97.0 noncon
hsa-miR-4749-3p 1034-1041 -0.455 98.0 -0.376 97.0 noncon
hsa-miR-5008-5p 47-54 -0.452 98.0 -0.452 99.0 noncon
hsa-miR-6734-5p 462-469 -0.445 98.0 -0.445 98.0 noncon
hsa-miR-4795-5p 146-153 -0.431 98.0 -0.431 99.0 noncon
hsa-miR-3180-3p 305-311 -0.429 92.0 -0.429 93.0 noncon
hsa-miR-3180 305-311 -0.429 92.0 -0.429 93.0 noncon
hsa-miR-6887-5p 442-448 -0.429 93.0 -0.429 95.0 noncon
hsa-miR-3175 484-491 -0.424 98.0 -0.424 98.0 noncon
hsa-miR-3196 305-311 -0.415 90.0 -0.415 92.0 noncon
hsa-miR-6795-5p 442-448 -0.407 92.0 -0.407 95.0 noncon
hsa-miR-6816-5p 305-311 -0.407 90.0 -0.407 91.0 noncon
hsa-miR-376b-3p 1103-1110 -0.404 98.0 -0.335 98.0 con
hsa-miR-376a-3p 1103-1110 -0.404 98.0 -0.335 98.0 con
hsa-miR-486-3p 997-1004 -0.403 95.0 -0.334 93.0 noncon
hsa-miR-6762-3p 522-529 -0.389 97.0 -0.389 98.0 noncon
hsa-miR-6506-3p 1088-1094 -0.384 93.0 -0.319 91.0 noncon
hsa-miR-3202 309-316 -0.373 98.0 -0.373 98.0 noncon
hsa-miR-6799-3p 44-50 -0.37 97.0 -0.37 98.0 noncon
hsa-miR-6805-5p 617-623 -0.365 94.0 -0.365 96.0 noncon
hsa-miR-3689c 936-943 -0.363 97.0 -0.302 96.0 noncon
hsa-miR-3689b-3p 936-943 -0.363 97.0 -0.302 96.0 noncon
hsa-miR-30b-3p 936-943 -0.363 97.0 -0.302 96.0 noncon
hsa-miR-7847-3p 660-666 -0.357 96.0 -0.357 97.0 noncon
hsa-miR-3689a-3p 936-943 -0.355 97.0 -0.295 96.0 noncon
hsa-miR-20b-3p 502-509 -0.355 98.0 -0.355 98.0 noncon
hsa-miR-6779-5p 936-943 -0.355 97.0 -0.295 96.0 noncon
hsa-miR-625-5p 679-685 -0.352 94.0 -0.352 96.0 noncon
hsa-miR-628-5p 60-67 -0.348 98.0 -0.348 98.0 noncon
hsa-miR-1273h-5p 936-943 -0.347 96.0 -0.289 96.0 noncon
hsa-miR-4512 235-242 -0.337 97.0 -0.337 98.0 noncon
hsa-miR-6848-5p 616-622 -0.335 91.0 -0.335 93.0 noncon
hsa-miR-6846-5p 616-622 -0.335 91.0 -0.335 93.0 noncon
hsa-miR-6780a-5p 936-943 -0.334 97.0 -0.278 96.0 noncon
hsa-miR-765 651-658 -0.332 96.0 -0.332 97.0 noncon
hsa-miR-3677-5p 478-484 -0.326 97.0 -0.326 98.0 noncon
hsa-miR-4671-5p 348-354 -0.326 93.0 -0.326 94.0 noncon
hsa-miR-5591-5p 1025-1031 -0.325 95.0 -0.271 94.0 noncon
hsa-miR-3171 284-291 -0.323 96.0 -0.323 97.0 noncon
hsa-miR-6515-5p 659-665 -0.318 96.0 -0.318 97.0 noncon
hsa-miR-7159-5p 1149-1156 -0.315 98.0 -0.262 98.0 noncon
hsa-miR-3173-5p 44-50 -0.315 96.0 -0.315 97.0 noncon
hsa-miR-4697-5p 364-370 -0.314 94.0 -0.314 95.0 noncon
hsa-miR-4259 684-690 -0.313 95.0 -0.313 97.0 noncon
hsa-miR-514a-5p 807-814 -0.312 98.0 -0.26 97.0 noncon
hsa-miR-7851-3p 775-781 -0.312 96.0 -0.26 95.0 noncon
hsa-miR-4284 1051-1058 -0.312 98.0 -0.26 98.0 noncon
hsa-miR-3616-5p 147-154 -0.304 97.0 -0.304 98.0 noncon
hsa-miR-4650-5p 972-979 -0.303 97.0 -0.253 97.0 noncon
hsa-miR-891a-3p 832-838 -0.302 96.0 -0.252 96.0 noncon
hsa-miR-887-5p 937-944 -0.3 96.0 -0.25 96.0 noncon
hsa-miR-4278 444-450 -0.299 90.0 -0.299 93.0 noncon
hsa-miR-4758-5p 663-669 -0.298 97.0 -0.298 98.0 noncon
hsa-miR-5572 277-284 -0.297 97.0 -0.297 98.0 noncon
hsa-miR-6763-5p 485-491 -0.296 95.0 -0.296 96.0 noncon
hsa-miR-3192-5p 583-590 -0.295 98.0 -0.295 98.0 noncon
hsa-miR-6880-5p 654-660 -0.292 93.0 -0.292 94.0 noncon
hsa-miR-4665-5p 682-688 -0.292 92.0 -0.292 94.0 noncon
hsa-miR-6827-5p 632-639 -0.291 91.0 -0.291 93.0 noncon
hsa-miR-573 147-154 -0.291 97.0 -0.291 98.0 noncon
hsa-miR-6726-5p 1025-1031 -0.289 93.0 -0.241 92.0 noncon
hsa-miR-132-3p 1124-1130 -0.288 98.0 -0.24 97.0 noncon
hsa-miR-212-3p 1124-1130 -0.288 98.0 -0.24 97.0 noncon
hsa-miR-3138 143-149 -0.286 90.0 -0.286 93.0 noncon
hsa-miR-520f-5p 862-869 -0.283 98.0 -0.236 97.0 noncon
hsa-miR-4642 429-436 -0.282 95.0 -0.282 96.0 noncon
hsa-miR-6798-5p 400-406 -0.282 94.0 -0.282 96.0 noncon
hsa-miR-4300 1025-1031 -0.281 93.0 -0.235 92.0 noncon
hsa-miR-604 523-529 -0.281 97.0 -0.281 97.0 noncon
hsa-miR-637 723-729 -0.28 95.0 -0.28 96.0 noncon
hsa-miR-6804-5p 657-663 -0.278 90.0 -0.278 92.0 noncon
hsa-miR-4488 364-370 -0.278 90.0 -0.278 92.0 noncon
hsa-miR-1237-5p 364-370 -0.274 90.0 -0.274 92.0 noncon
hsa-miR-1291 45-51 -0.271 91.0 -0.271 93.0 noncon
hsa-miR-6797-5p 648-654 -0.27 94.0 -0.27 95.0 noncon
hsa-miR-4750-3p 321-327 -0.269 95.0 -0.269 97.0 noncon
hsa-miR-6849-3p 31-38 -0.267 97.0 -0.267 98.0 noncon
hsa-miR-3150a-3p 485-491 -0.263 94.0 -0.263 95.0 noncon
hsa-miR-6745 655-661 -0.26 93.0 -0.26 95.0 noncon
hsa-miR-4725-3p 986-992 -0.259 91.0 -0.216 90.0 noncon
hsa-miR-4314 584-591 -0.256 93.0 -0.256 95.0 noncon
hsa-miR-1249-5p 659-665 -0.255 92.0 -0.255 94.0 noncon
hsa-miR-6721-5p 996-1002 -0.255 91.0 -0.213 89.0 noncon
hsa-miR-1238-5p 663-669 -0.254 96.0 -0.254 97.0 noncon
hsa-miR-4642 567-573 -0.254 94.0 -0.254 96.0 noncon
hsa-miR-3612 803-809 -0.252 94.0 -0.211 93.0 noncon
hsa-miR-6743-5p 460-466 -0.252 92.0 -0.252 94.0 noncon
hsa-miR-7847-3p 653-659 -0.251 90.0 -0.251 92.0 noncon