GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Glycogene class
ABO
ALG1
ALG10
ALG10B
ALG11
ALG12
ALG13
ALG14
ALG1L
ALG1L2
ALG2
ALG3
ALG5
ALG6
ALG8
ALG9
B3GALNT2
B3GALT2
B3GAT1
B3GAT2
B3GNT2
B3GNT4
B3GNT7
B3GNT8
B4GALNT2
B4GALNT3
B4GALNT4
B4GALT1
B4GALT2
B4GALT3
B4GALT4
B4GALT5
CHST1
CHST10
CHST2
CHST3
CHST4
CHST5
CHST8
CHST9
CMAS
CTBS
DAD1
DDOST
DPAGT1
DPM1
DPM2
DPM3
EDEM1
EDEM2
EDEM3
ENGASE
FPGT
FUCA1
FUT1
FUT10
FUT11
FUT2
FUT3
FUT4
FUT5
FUT6
FUT7
FUT8
FUT9
GAL3ST2
GAL3ST3
GANAB
GCNT2
GNPNAT1
GNPTAB
GNPTG
MAN1A1
MAN1A2
MAN1B1
MAN1C1
MAN2A1
MAN2A2
MAN2B1
MAN2B2
MAN2C1
MANBA
MANEA
MGAT1
MGAT2
MGAT3
MGAT4A
MGAT4B
MGAT4C
MGAT4D
MGAT5
MOGS
NAGK
NAGPA
NEU1
NEU2
NEU3
NEU4
PRKCSH
RPN1
RPN2
ST3GAL3
ST3GAL4
ST3GAL6
ST6GAL1
ST6GAL2
ST8SIA2
ST8SIA3
ST8SIA4
STT3A
STT3B
UGGT1
UGGT2
Pathway Map
NAGK



General Information
Gene Symbol:   
NAGK
Protein name:   
N-acetyl-D-glucosamine kinase
Enzyme Class:   
CAZy Family:   
E.C. No:   
2.7.1.59
Tissues:   
all, Muscular, Epithelial, Connective, Nervous
Organs:   
Reaction (SNFG):   

GlcNAc → GlcNAc(6P)
Reaction (IUBMB):   
No specified reaction.
About

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-446210
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9UJ70 
NCBI GeneID:   
55577
GlyMap GeneID:   
55577
DNA aRefSeq:   
NM_017567.4 
Protein RefSeq:   
NP_060037.3 
BRENDA:   
Kinetic info.
OMIM number:   
606828
GeneCards database:   
NAGK
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     
Substrate:  
     GlcNAc
Product:  
     GlcNAc(6P)
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 160 140 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-519d-3p 114-121 -0.753 99.0 -0.753 99.0 con
hsa-miR-20b-5p 114-121 -0.729 99.0 -0.729 99.0 con
hsa-miR-17-5p 114-121 -0.729 99.0 -0.729 99.0 con
hsa-miR-106a-5p 114-121 -0.726 99.0 -0.726 99.0 con
hsa-miR-93-5p 114-121 -0.721 99.0 -0.721 99.0 con
hsa-miR-20a-5p 114-121 -0.716 99.0 -0.716 99.0 con
hsa-miR-526b-3p 114-121 -0.688 99.0 -0.688 99.0 con
hsa-miR-106b-5p 114-121 -0.684 99.0 -0.684 99.0 con
hsa-miR-8068 91-98 -0.581 99.0 -0.581 99.0 noncon
hsa-miR-6795-5p 315-322 -0.574 97.0 -0.035 29.0 noncon
hsa-miR-6887-5p 315-322 -0.574 97.0 -0.035 28.0 noncon
hsa-miR-6515-5p 22-29 -0.548 99.0 -0.548 99.0 noncon
hsa-miR-4659b-3p 100-107 -0.534 99.0 -0.534 99.0 noncon
hsa-miR-4659a-3p 100-107 -0.534 99.0 -0.534 99.0 noncon
hsa-miR-4792 432-439 -0.494 99.0 -0.031 61.0 noncon
hsa-miR-1304-5p 1269-1276 -0.482 99.0 -0.03 65.0 noncon
hsa-miR-6763-5p 126-133 -0.46 99.0 -0.029 55.0 noncon
hsa-miR-3150a-3p 126-133 -0.452 98.0 -0.029 56.0 noncon
hsa-miR-6769a-3p 506-513 -0.435 99.0 -0.028 69.0 noncon
hsa-miR-3663-3p 246-253 -0.435 97.0 -0.028 53.0 noncon
hsa-miR-519c-3p 113-119 -0.434 99.0 -0.434 99.0 noncon
hsa-miR-519a-3p 113-119 -0.434 99.0 -0.434 99.0 noncon
hsa-miR-519b-3p 113-119 -0.434 99.0 -0.434 99.0 noncon
hsa-miR-1286 924-931 -0.421 99.0 -0.027 66.0 noncon
hsa-miR-181a-2-3p 32-39 -0.419 99.0 -0.419 99.0 noncon
hsa-miR-3681-5p 121-127 -0.403 99.0 -0.403 99.0 noncon
hsa-miR-1827 1267-1274 -0.397 98.0 -0.026 54.0 noncon
hsa-miR-3605-5p 167-174 -0.39 97.0 -0.025 51.0 noncon
hsa-miR-376b-5p 120-126 -0.38 97.0 -0.38 98.0 noncon
hsa-miR-7109-5p 316-322 -0.359 96.0 -0.023 47.0 noncon
hsa-miR-608 17-23 -0.35 91.0 -0.35 93.0 noncon
hsa-miR-6828-5p 67-73 -0.347 97.0 -0.347 98.0 noncon
hsa-miR-6849-5p 121-127 -0.341 99.0 -0.341 99.0 noncon
hsa-miR-6742-3p 1195-1202 -0.34 98.0 -0.022 68.0 noncon
hsa-miR-6748-3p 860-867 -0.339 96.0 -0.022 60.0 noncon
hsa-miR-6790-3p 1055-1062 -0.337 98.0 -0.022 56.0 noncon
hsa-miR-6752-5p 18-24 -0.334 91.0 -0.334 93.0 noncon
hsa-miR-376c-5p 120-126 -0.332 97.0 -0.332 98.0 noncon
hsa-miR-4428 76-82 -0.332 98.0 -0.332 99.0 noncon
hsa-miR-6732-5p 19-25 -0.323 95.0 -0.323 96.0 noncon
hsa-miR-7159-3p 97-103 -0.321 99.0 -0.321 99.0 noncon
hsa-miR-4752 874-881 -0.32 97.0 -0.021 52.0 noncon
hsa-miR-3667-3p 53-60 -0.313 97.0 -0.313 97.0 noncon
hsa-miR-3692-5p 184-191 -0.307 97.0 -0.02 61.0 noncon
hsa-miR-1976 1178-1185 -0.306 97.0 -0.02 74.0 noncon
hsa-miR-4268 745-752 -0.298 96.0 -0.02 62.0 noncon
hsa-miR-1261 119-125 -0.293 96.0 -0.293 97.0 noncon
hsa-miR-6755-5p 308-314 -0.283 91.0 -0.019 39.0 noncon
hsa-miR-6804-5p 476-482 -0.281 90.0 -0.019 35.0 noncon
hsa-miR-4531 938-945 -0.281 97.0 -0.019 44.0 noncon
hsa-miR-1537-3p 984-990 -0.277 90.0 -0.019 29.0 noncon
hsa-miR-412-3p 848-855 -0.275 96.0 -0.018 57.0 noncon
hsa-miR-1193 363-369 -0.271 95.0 -0.018 62.0 noncon
hsa-miR-27b-5p 28-34 -0.271 95.0 -0.271 97.0 noncon
hsa-miR-1249-5p 22-28 -0.271 94.0 -0.271 95.0 noncon
hsa-miR-5589-3p 154-160 -0.27 96.0 -0.018 49.0 noncon
hsa-miR-504-3p 1237-1244 -0.269 95.0 -0.018 63.0 noncon
hsa-miR-370-5p 363-369 -0.268 96.0 -0.018 63.0 noncon
hsa-miR-1233-3p 200-207 -0.266 95.0 -0.018 43.0 noncon
hsa-miR-4288 763-769 -0.265 95.0 -0.018 61.0 noncon
hsa-miR-517-5p 964-970 -0.265 95.0 -0.018 44.0 noncon
hsa-miR-6071 418-425 -0.263 96.0 -0.018 51.0 noncon
hsa-miR-4736 179-186 -0.263 96.0 -0.018 48.0 noncon
hsa-miR-7113-3p 452-458 -0.263 96.0 -0.018 54.0 noncon
hsa-miR-6832-5p 1102-1108 -0.262 92.0 -0.018 39.0 noncon
hsa-miR-6747-3p 1176-1183 -0.261 95.0 -0.018 54.0 noncon
hsa-miR-3175 126-132 -0.26 92.0 -0.018 31.0 noncon
hsa-miR-455-3p.2 137-143 -0.26 97.0 -0.018 74.0 noncon
hsa-miR-6797-5p 22-28 -0.259 93.0 -0.259 95.0 noncon
hsa-miR-660-3p 1198-1204 -0.258 96.0 -0.017 50.0 noncon
hsa-miR-3130-5p 41-47 -0.258 97.0 -0.258 98.0 noncon
hsa-miR-5684 965-971 -0.256 96.0 -0.017 50.0 noncon
hsa-miR-4722-3p 1177-1183 -0.255 95.0 -0.017 44.0 noncon
hsa-miR-5684 511-518 -0.254 96.0 -0.017 50.0 noncon
hsa-miR-6132 234-240 -0.252 92.0 -0.017 44.0 noncon