GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Glycogene class
A3GALT2
A4GALT
A4GNT
ABO
ALG1
ALG10
ALG10B
ALG11
ALG12
ALG13
ALG14
ALG1L
ALG1L2
ALG2
ALG3
ALG5
ALG6
ALG8
ALG9
B3GALNT1
B3GALNT2
B3GALT1
B3GALT2
B3GALT4
B3GALT5
B3GAT1
B3GAT2
B3GNT2
B3GNT3
B3GNT4
B3GNT5
B3GNT6
B3GNT7
B3GNT8
B4GALNT1
B4GALNT2
B4GALNT3
B4GALNT4
B4GALT1
B4GALT2
B4GALT3
B4GALT4
B4GALT5
B4GALT6
C1GALT1
C1GALT1C1
CHST1
CHST10
CHST2
CHST3
CHST4
CHST5
CHST8
CHST9
CMAS
CTBS
DAD1
DDOST
DPAGT1
DPM1
DPM2
DPM3
EDEM1
EDEM2
EDEM3
ENGASE
FPGT
FUCA1
FUT1
FUT10
FUT11
FUT2
FUT3
FUT4
FUT5
FUT6
FUT7
FUT8
FUT9
GAL3ST1
GAL3ST2
GAL3ST3
GAL3ST4
GALNT1
GALNT10
GALNT11
GALNT12
GALNT13
GALNT14
GALNT15
GALNT16
GALNT17
GALNT18
GALNT2
GALNT3
GALNT4
GALNT5
GALNT6
GALNT7
GALNT8
GALNT9
GALNTL6
GANAB
GBGT1
GCNT1
GCNT2
GCNT3
GCNT4
GNPNAT1
GNPTAB
GNPTG
HEXA
HEXB
MAN1A1
MAN1A2
MAN1B1
MAN1C1
MAN2A1
MAN2A2
MAN2B1
MAN2B2
MAN2C1
MANBA
MANEA
MGAT1
MGAT2
MGAT3
MGAT4A
MGAT4B
MGAT4C
MGAT4D
MGAT5
MOGS
NAGA
NAGK
NAGPA
NEU1
NEU2
NEU3
NEU4
PRKCSH
RPN1
RPN2
ST3GAL1
ST3GAL2
ST3GAL3
ST3GAL4
ST3GAL5
ST3GAL6
ST6GAL1
ST6GAL2
ST6GALNAC1
ST6GALNAC2
ST6GALNAC3
ST6GALNAC4
ST6GALNAC5
ST6GALNAC6
ST8SIA1
ST8SIA2
ST8SIA3
ST8SIA4
ST8SIA5
ST8SIA6
STT3A
STT3B
UGCG
UGGT1
UGGT2
UGT8
Pathway Map
B4GALNT2



General Information
Gene Symbol:   
B4GALNT2
Protein name:   
Beta-1,4 N-acetylgalactosaminyltransferase 2
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT12
E.C. No:   
2.4.1.*
Organs:   
Reaction (SNFG):   
+
→
+ UDP
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• Activity is similar to B4GalNT1, but substrate specificity is distinct as it forms the Sda or Cad antigen.
• B4GalNT2 is suppressed during gastric cancer.

Related Pathway
KEGG ORTHOLOGY:   
K09655
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-446203
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q8NHY0 
NCBI GeneID:   
124872
GlyMap GeneID:   
124872
DNA aRefSeq:   
NM_001159387.1 
Protein RefSeq:   
NP_001152859.1 
BRENDA:   
Kinetic info.
OMIM number:   
111730
GeneCards database:   
B4GALNT2
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     NeuAc(a2-3)Gal(b1-?)GlcNAc(b1-?) or NeuAc(a2-3)Gal(b1-?)GalNAc(a1-?)
Product:  
     NeuAc(a2-3)[GalNAc(b1-4)]Gal(b1-?)GlcNAc(b1-?) or NeuAc(a2-3)[GalNAc(b1-4)]Gal(b1-?)GalNAc(a1-?)
Constraints:  
     !Fuc(a1-2)@Gal(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.70e-06    (pmol/cell • hr)       KM:    7.00e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 6 4 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-509-3p 145-152 -0.573 99.0 -0.573 99.0 noncon
hsa-miR-3162-3p 109-116 -0.564 99.0 -0.564 99.0 noncon
hsa-miR-874-3p 190-197 -0.562 99.0 -0.562 99.0 noncon
hsa-miR-150-3p 90-97 -0.504 98.0 -0.504 99.0 noncon
hsa-let-7c-3p 226-233 -0.459 99.0 -0.459 99.0 noncon
hsa-miR-4433a-3p 71-78 -0.455 99.0 -0.455 99.0 noncon
hsa-miR-6132 235-241 -0.436 98.0 -0.436 98.0 noncon
hsa-miR-5190 181-188 -0.427 99.0 -0.427 99.0 noncon
hsa-miR-6836-5p 235-241 -0.421 98.0 -0.421 98.0 noncon
hsa-miR-1289 69-75 -0.417 97.0 -0.417 98.0 noncon
hsa-miR-1207-5p 236-242 -0.416 98.0 -0.416 99.0 noncon
hsa-miR-4755-5p 118-125 -0.411 99.0 -0.411 99.0 noncon
hsa-miR-5006-3p 118-125 -0.411 99.0 -0.411 99.0 noncon
hsa-miR-4763-3p 236-242 -0.406 98.0 -0.406 98.0 noncon
hsa-miR-204-5p 117-124 -0.404 98.0 -0.404 99.0 noncon
hsa-miR-211-5p 117-124 -0.396 98.0 -0.396 98.0 noncon
hsa-miR-6751-3p 127-133 -0.395 98.0 -0.395 99.0 noncon
hsa-miR-1199-5p 127-133 -0.391 98.0 -0.391 99.0 noncon
hsa-miR-136-5p 196-203 -0.354 98.0 -0.354 98.0 noncon
hsa-miR-6808-5p 237-243 -0.346 99.0 -0.346 99.0 noncon
hsa-miR-6893-5p 237-243 -0.346 99.0 -0.346 99.0 noncon
hsa-miR-1256 231-237 -0.342 97.0 -0.342 98.0 noncon
hsa-miR-6832-3p 116-123 -0.338 98.0 -0.338 98.0 noncon
hsa-miR-940 237-243 -0.328 99.0 -0.328 99.0 noncon
hsa-miR-4446-5p 112-119 -0.318 98.0 -0.318 98.0 noncon
hsa-miR-7973 161-167 -0.317 94.0 -0.317 96.0 noncon
hsa-miR-4317 193-199 -0.301 95.0 -0.301 96.0 noncon
hsa-miR-3977 248-254 -0.291 98.0 -0.291 98.0 noncon
hsa-miR-3157-3p 191-197 -0.285 97.0 -0.285 98.0 noncon
hsa-miR-6754-3p 155-161 -0.284 96.0 -0.284 97.0 noncon
hsa-miR-4761-3p 233-239 -0.278 98.0 -0.278 98.0 noncon
hsa-miR-134-5p 179-185 -0.273 94.0 -0.273 96.0 noncon
hsa-miR-105-5p 252-258 -0.273 98.0 -0.273 99.0 noncon
hsa-miR-7853-5p 252-258 -0.273 98.0 -0.273 99.0 noncon
hsa-miR-4446-5p 119-125 -0.269 97.0 -0.269 97.0 noncon
hsa-miR-374c-5p 205-212 -0.268 96.0 -0.268 97.0 noncon
hsa-miR-1226-3p 102-108 -0.268 95.0 -0.268 97.0 noncon
hsa-miR-3194-5p 28-34 -0.266 94.0 -0.266 95.0 noncon
hsa-miR-412-3p 155-161 -0.263 95.0 -0.263 97.0 noncon
hsa-miR-1263 143-149 -0.263 90.0 -0.263 93.0 noncon
hsa-miR-655-3p 205-212 -0.26 96.0 -0.26 97.0 noncon
hsa-miR-802 133-139 -0.254 96.0 -0.254 97.0 noncon
hsa-miR-4768-3p 72-78 -0.254 98.0 -0.254 98.0 noncon
hsa-miR-3118 179-185 -0.251 92.0 -0.251 94.0 noncon