GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Glycogene class
ABO
ALG1
ALG10
ALG10B
ALG11
ALG12
ALG13
ALG14
ALG1L
ALG1L2
ALG2
ALG3
ALG5
ALG6
ALG8
ALG9
B3GALNT2
B3GALT2
B3GAT1
B3GAT2
B3GNT2
B3GNT4
B3GNT7
B3GNT8
B4GALNT2
B4GALNT3
B4GALNT4
B4GALT1
B4GALT2
B4GALT3
B4GALT4
B4GALT5
CHST1
CHST10
CHST2
CHST3
CHST4
CHST5
CHST8
CHST9
CMAS
CTBS
DAD1
DDOST
DPAGT1
DPM1
DPM2
DPM3
EDEM1
EDEM2
EDEM3
ENGASE
FPGT
FUCA1
FUT1
FUT10
FUT11
FUT2
FUT3
FUT4
FUT5
FUT6
FUT7
FUT8
FUT9
GAL3ST2
GAL3ST3
GANAB
GCNT2
GNPNAT1
GNPTAB
GNPTG
MAN1A1
MAN1A2
MAN1B1
MAN1C1
MAN2A1
MAN2A2
MAN2B1
MAN2B2
MAN2C1
MANBA
MANEA
MGAT1
MGAT2
MGAT3
MGAT4A
MGAT4B
MGAT4C
MGAT4D
MGAT5
MOGS
NAGK
NAGPA
NEU1
NEU2
NEU3
NEU4
PRKCSH
RPN1
RPN2
ST3GAL3
ST3GAL4
ST3GAL6
ST6GAL1
ST6GAL2
ST8SIA2
ST8SIA3
ST8SIA4
STT3A
STT3B
UGGT1
UGGT2
Pathway Map
FUT10



General Information
Gene Symbol:   
FUT10
Protein name:   
Alpha-(1,3)-fucosyltransferase 10
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT10
E.C. No:   
2.4.1.152
Organs:   
Reaction (SNFG):   
+
→
+ GDP
Reaction (IUBMB):   
No specified reaction.
About

• FUT 10 and 11 are invertebrate and plant core α3-fucosyltransferases that are expressed in the ER (and not Golgi).
• They attach α(1,3)Fuc to the innermost core GlcNAc of the N-glycans on conalbumin glycopeptide substrates and also synthetic biantennary N-glycans.
• It is generally accepted that the core α3-fucosylation mediated by these enzymes does not occur in humans.
• However the wide spread expression of core α(1,3)Fuc in pollen, foods, parasites and insect venom may result in IgE dependent allergic reactions in humans.

Related Pathway
KEGG ORTHOLOGY:   
K09669
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-9037629
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q6P4F1 
NCBI GeneID:   
84750
GlyMap GeneID:   
84750
DNA aRefSeq:   
NM_032664.3 
Protein RefSeq:   
NP_116053.3 
BRENDA:   
Kinetic info.
OMIM number:   
-
GeneCards database:   
FUT10
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     ER
Substrate:  
     ...GlcNAc(b1-?)Asn
Product:  
     Fuc(a1-3)...GlcNAc(b1-?)Asn
Constraints:  
     nGlcNAc(b1-2)Man(a1-3)[Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc(b1-?)Asn>=1&!GlcNAc(b1-4)...Man(b1-4)&!Fuc(a1-6)...GlcNAc(b1-?)Asn
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.80e-05    (pmol/cell • hr)       KM:    2.50e+07    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000327671 ENST00000416169 ENST00000518672 ENST00000520767 RUNX1 ZNF263 ZNF770 ZBTB33 NFE2 ZNF143 ZNF263
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-124-3p.2 59-66 -0.553 99.0 -0.553 99.0 con
hsa-miR-7848-3p 1555-1562 -0.483 98.0 -0.148 86.0 noncon
hsa-miR-506-3p 59-66 -0.477 99.0 -0.477 99.0 con
hsa-miR-6798-3p 4730-4737 -0.447 96.0 -0.007 30.0 noncon
hsa-miR-4640-3p 4729-4736 -0.441 98.0 -0.007 25.0 noncon
hsa-miR-4317 431-438 -0.431 98.0 -0.431 98.0 noncon
hsa-miR-4480 427-434 -0.412 98.0 -0.412 99.0 noncon
hsa-miR-3680-5p 470-477 -0.412 99.0 -0.412 99.0 noncon
hsa-miR-566 3574-3581 -0.406 97.0 -0.006 25.0 noncon
hsa-miR-4693-3p 354-361 -0.404 98.0 -0.404 98.0 noncon
hsa-miR-605-3p 1856-1863 -0.395 98.0 -0.092 72.0 noncon
hsa-miR-4780 1592-1599 -0.389 98.0 -0.108 81.0 noncon
hsa-miR-4418 121-128 -0.374 99.0 -0.374 99.0 noncon
hsa-miR-6792-3p 1611-1618 -0.372 98.0 -0.104 79.0 noncon
hsa-miR-6835-5p 783-790 -0.372 96.0 -0.117 66.0 noncon
hsa-miR-5195-5p 170-177 -0.369 98.0 -0.369 98.0 noncon
hsa-miR-7854-3p 348-355 -0.367 98.0 -0.367 99.0 noncon
hsa-miR-6731-3p 149-156 -0.365 98.0 -0.365 98.0 noncon
hsa-miR-3681-5p 498-505 -0.363 98.0 -0.114 88.0 noncon
hsa-miR-509-3-5p 121-128 -0.361 99.0 -0.361 99.0 noncon
hsa-miR-509-5p 121-128 -0.345 99.0 -0.345 99.0 noncon
hsa-miR-5088-5p 137-143 -0.342 97.0 -0.342 98.0 noncon
hsa-miR-4308 458-465 -0.338 98.0 -0.338 98.0 noncon
hsa-miR-3134 97-104 -0.337 97.0 -0.337 98.0 noncon
hsa-miR-6864-5p 614-621 -0.335 97.0 -0.106 83.0 noncon
hsa-miR-4256 1728-1734 -0.334 96.0 -0.092 80.0 noncon
hsa-miR-4474-3p 1077-1084 -0.334 96.0 -0.106 81.0 noncon
hsa-miR-644a 2845-2852 -0.328 96.0 -0.005 41.0 noncon
hsa-miR-6856-5p 1963-1970 -0.327 98.0 -0.078 83.0 noncon
hsa-miR-660-5p 1630-1637 -0.321 96.0 -0.091 82.0 noncon
hsa-miR-7515 613-620 -0.32 97.0 -0.102 78.0 noncon
hsa-miR-6758-5p 1963-1970 -0.317 98.0 -0.076 82.0 noncon
hsa-miR-450b-3p 441-447 -0.314 97.0 -0.314 98.0 noncon
hsa-miR-1184 120-126 -0.313 98.0 -0.313 98.0 noncon
hsa-miR-6748-3p 1104-1111 -0.311 95.0 -0.099 80.0 noncon
hsa-miR-5703 1844-1851 -0.308 97.0 -0.074 78.0 noncon
hsa-miR-7154-5p 1912-1919 -0.307 98.0 -0.073 79.0 noncon
hsa-miR-652-3p 3576-3582 -0.303 90.0 -0.005 22.0 noncon
hsa-miR-4437 136-142 -0.3 94.0 -0.3 96.0 noncon
hsa-miR-5196-3p 1643-1650 -0.3 97.0 -0.085 84.0 noncon
hsa-miR-299-3p 254-261 -0.298 96.0 -0.298 97.0 noncon
hsa-miR-650 4518-4525 -0.298 97.0 -0.005 28.0 noncon
hsa-miR-892b 4858-4864 -0.296 95.0 -0.005 27.0 noncon
hsa-miR-6849-5p 498-505 -0.295 98.0 -0.094 89.0 noncon
hsa-miR-6870-3p 1328-1335 -0.294 97.0 -0.094 88.0 noncon
hsa-miR-6759-3p 1725-1732 -0.29 95.0 -0.081 81.0 noncon
hsa-miR-4443 4519-4526 -0.289 95.0 -0.005 39.0 noncon
hsa-miR-3667-3p 1614-1621 -0.288 96.0 -0.082 81.0 noncon
hsa-miR-4662b 1928-1935 -0.287 98.0 -0.069 83.0 noncon
hsa-miR-4434 1844-1851 -0.284 97.0 -0.068 78.0 noncon
hsa-miR-4691-5p 1611-1618 -0.283 98.0 -0.081 82.0 noncon
hsa-miR-3612 4518-4525 -0.282 96.0 -0.005 27.0 noncon
hsa-miR-597-3p 1281-1288 -0.277 94.0 -0.089 81.0 noncon
hsa-miR-4798-3p 471-477 -0.273 97.0 -0.273 98.0 noncon
hsa-miR-5681a 140-147 -0.273 97.0 -0.273 97.0 noncon
hsa-miR-4434 546-553 -0.272 96.0 -0.088 82.0 noncon
hsa-miR-379-5p 537-543 -0.268 94.0 -0.086 80.0 noncon
hsa-miR-6502-5p 28-34 -0.268 93.0 -0.268 95.0 noncon
hsa-miR-3200-3p 1816-1823 -0.267 95.0 -0.064 82.0 noncon
hsa-miR-3173-3p 1298-1305 -0.267 96.0 -0.086 80.0 noncon
hsa-miR-6852-5p 786-793 -0.266 96.0 -0.086 81.0 noncon
hsa-miR-1301-5p 28-34 -0.264 94.0 -0.264 95.0 noncon
hsa-miR-6822-3p 29-35 -0.259 94.0 -0.259 96.0 noncon
hsa-miR-34b-5p 265-271 -0.258 97.0 -0.258 98.0 noncon
hsa-miR-449c-5p 265-271 -0.258 97.0 -0.258 98.0 noncon
hsa-miR-3529-5p 537-543 -0.258 93.0 -0.083 77.0 noncon
hsa-miR-769-3p 441-447 -0.256 96.0 -0.256 97.0 noncon
hsa-miR-2682-5p 265-271 -0.255 97.0 -0.255 98.0 noncon
hsa-miR-192-5p 4542-4549 -0.253 96.0 -0.004 35.0 noncon
hsa-miR-4638-3p 3496-3503 -0.252 92.0 -0.004 27.0 noncon
hsa-miR-584-3p 1574-1580 -0.252 94.0 -0.072 81.0 noncon
hsa-miR-6768-5p 1488-1495 -0.251 97.0 -0.081 87.0 noncon