GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Glycogene class
A3GALT2
A4GALT
A4GNT
ABO
B3GALNT1
B3GALNT2
B3GALT1
B3GALT2
B3GALT4
B3GALT5
B3GAT1
B3GAT2
B3GNT2
B3GNT3
B3GNT4
B3GNT5
B3GNT6
B3GNT7
B4GALNT1
B4GALNT2
B4GALNT3
B4GALNT4
B4GALT1
B4GALT2
B4GALT3
B4GALT4
B4GALT5
B4GALT6
C1GALT1
C1GALT1C1
CHST1
CHST10
CHST2
CHST3
CHST4
CHST5
CHST8
CHST9
CMAS
FPGT
FUT1
FUT2
FUT3
FUT4
FUT5
FUT6
FUT7
FUT9
GAL3ST1
GAL3ST2
GAL3ST3
GAL3ST4
GALNT1
GALNT10
GALNT11
GALNT12
GALNT13
GALNT14
GALNT15
GALNT16
GALNT17
GALNT18
GALNT2
GALNT3
GALNT4
GALNT5
GALNT6
GALNT7
GALNT8
GALNT9
GALNTL6
GBGT1
GCNT1
GCNT2
GCNT3
GCNT4
HEXA
HEXB
NAGA
NEU1
NEU2
NEU3
NEU4
ST3GAL1
ST3GAL2
ST3GAL3
ST3GAL4
ST3GAL5
ST3GAL6
ST6GAL1
ST6GAL2
ST6GALNAC1
ST6GALNAC2
ST6GALNAC3
ST6GALNAC4
ST6GALNAC5
ST6GALNAC6
ST8SIA1
ST8SIA2
ST8SIA3
ST8SIA4
ST8SIA5
ST8SIA6
UGCG
UGT8
Pathway Map
B3GALT5



General Information
Gene Symbol:   
B3GALT5
Protein name:   
Beta-1,3-galactosyltransferase 5
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT31
E.C. No:   
2.4.1.*
Organs:   
Reaction (SNFG):   
+
→
+ UDP
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• Produces type 1 chain linkage in glycolipids and core-3 O-glycans, but not N-linked glycoproteins.
• May be an important enzyme forming CA19-9 in colon cancer. Beyond serving as a biomarker, CA19-9 also promotes pancreatitis and pancreatic cancer (in mice).
• B3GalT5 efficiently transfers Gal to the GlcNAc of the glycolipid Lc3Cer and to the terminal GalNAc residue of the globoside Gb4.
• It may thus contribute to the formation of CA 19-9, SSEA-3 and elongated core-3 O-glycans.
• Expression is restricted to the colorectum, small intestine, stomach, and pancreas.

Related Pathway
KEGG ORTHOLOGY:   
K03877
KEGG Pathway:   
ko00601  ko00603  ko01100 
Reactome:   
R-HSA-9037629
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9Y2C3 
NCBI GeneID:   
10317
GlyMap GeneID:   
10317
DNA aRefSeq:   
NM_001278650.1 
Protein RefSeq:   
NP_001265579.1 
BRENDA:   
Kinetic info.
OMIM number:   
604066
GeneCards database:   
B3GALT5
Enzyme Specificity
Glycan Type:  
     O-linked (mucin type), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GlcNAc(b1-?) or GalNAc(b1-?)
Product:  
     Gal(b1-3)GlcNAc(b1-?) or Gal(b1-3)GalNAc(b1-?)
Constraints:  
     !Gal(b1-4)@GlcNAc(b1-?)&!GalNAc(b1-4)@GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 10 8 6 4 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-151a-5p 11594-11601 -0.559 98.0 -0.559 99.0 noncon
hsa-miR-151b 11594-11601 -0.559 98.0 -0.559 99.0 noncon
hsa-miR-425-3p 11151-11158 -0.482 98.0 -0.482 98.0 noncon
hsa-miR-6506-3p 11536-11543 -0.453 96.0 -0.453 97.0 noncon
hsa-miR-187-3p 10927-10934 -0.447 95.0 -0.447 97.0 noncon
hsa-miR-4763-5p 11452-11459 -0.409 95.0 -0.409 97.0 noncon
hsa-miR-6775-5p 4275-4281 -0.398 95.0 -0.398 96.0 noncon
hsa-miR-4266 5416-5423 -0.393 97.0 -0.393 98.0 noncon
hsa-miR-4266 1281-1287 -0.392 97.0 -0.392 98.0 noncon
hsa-miR-4706 659-666 -0.38 94.0 -0.38 95.0 noncon
hsa-miR-4749-5p 659-666 -0.37 94.0 -0.37 95.0 noncon
hsa-miR-5091 7319-7325 -0.368 92.0 -0.368 95.0 noncon
hsa-miR-7108-3p 75-82 -0.36 93.0 -0.36 95.0 noncon
hsa-miR-6749-5p 178-184 -0.352 93.0 -0.352 94.0 noncon
hsa-miR-5090 4275-4281 -0.351 92.0 -0.351 94.0 noncon
hsa-miR-6769b-3p 5281-5288 -0.335 97.0 -0.335 98.0 noncon
hsa-miR-6726-5p 511-518 -0.333 95.0 -0.333 96.0 noncon
hsa-miR-5591-5p 511-518 -0.32 95.0 -0.32 96.0 noncon
hsa-miR-4723-3p 5281-5288 -0.311 96.0 -0.311 97.0 noncon
hsa-miR-6812-5p 841-848 -0.305 94.0 -0.305 95.0 noncon
hsa-miR-4300 511-518 -0.296 94.0 -0.296 95.0 noncon
hsa-miR-4498 3613-3620 -0.292 92.0 -0.292 94.0 noncon
hsa-miR-4802-5p 10617-10623 -0.29 96.0 -0.29 97.0 noncon
hsa-miR-505-3p.1 11295-11302 -0.289 91.0 -0.289 94.0 noncon
hsa-miR-5001-5p 8651-8658 -0.289 92.0 -0.289 94.0 noncon
hsa-miR-4777-3p 7727-7734 -0.289 93.0 -0.289 95.0 noncon
hsa-miR-6819-5p 841-848 -0.287 93.0 -0.287 95.0 noncon
hsa-miR-5001-5p 3613-3620 -0.286 92.0 -0.286 93.0 noncon
hsa-miR-191-3p 10638-10645 -0.281 93.0 -0.281 95.0 noncon
hsa-miR-6737-5p 841-848 -0.279 93.0 -0.279 94.0 noncon
hsa-miR-4498 8651-8658 -0.279 91.0 -0.279 93.0 noncon
hsa-miR-660-5p 11029-11036 -0.275 95.0 -0.275 97.0 noncon
hsa-miR-323a-5p 6834-6841 -0.274 94.0 -0.274 95.0 noncon
hsa-miR-1915-5p 296-303 -0.274 91.0 -0.274 94.0 noncon
hsa-miR-4471 149-156 -0.271 93.0 -0.271 95.0 noncon
hsa-miR-4476 5947-5954 -0.27 97.0 -0.27 98.0 noncon
hsa-miR-6791-5p 1866-1872 -0.267 94.0 -0.267 96.0 noncon
hsa-miR-149-3p 7030-7036 -0.266 94.0 -0.266 95.0 noncon
hsa-miR-324-5p 2241-2248 -0.263 90.0 -0.263 93.0 noncon
hsa-miR-4663 5125-5132 -0.26 96.0 -0.26 97.0 noncon
hsa-miR-920 511-518 -0.26 95.0 -0.26 96.0 noncon
hsa-miR-1200 1404-1411 -0.257 96.0 -0.257 97.0 noncon
hsa-miR-6828-3p 10548-10555 -0.256 95.0 -0.256 96.0 noncon
hsa-miR-214-3p 10320-10326 -0.255 96.0 -0.255 97.0 noncon
hsa-miR-3606-5p 10849-10855 -0.255 93.0 -0.255 96.0 noncon
hsa-miR-6514-3p 293-300 -0.255 97.0 -0.255 98.0 noncon
hsa-miR-149-3p 1964-1971 -0.254 93.0 -0.254 94.0 noncon
hsa-miR-4728-5p 7030-7036 -0.253 93.0 -0.253 94.0 noncon
hsa-miR-1324 11633-11639 -0.25 96.0 -0.25 97.0 noncon
hsa-miR-1199-5p 733-740 -0.25 94.0 -0.25 96.0 noncon