GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Glycogene class
ABO
ALG1
ALG10
ALG10B
ALG11
ALG12
ALG13
ALG14
ALG1L
ALG1L2
ALG2
ALG3
ALG5
ALG6
ALG8
ALG9
B3GALNT2
B3GALT2
B3GAT1
B3GAT2
B3GNT2
B3GNT4
B3GNT7
B3GNT8
B4GALNT2
B4GALNT3
B4GALNT4
B4GALT1
B4GALT2
B4GALT3
B4GALT4
B4GALT5
CHST1
CHST10
CHST2
CHST3
CHST4
CHST5
CHST8
CHST9
CMAS
CTBS
DAD1
DDOST
DPAGT1
DPM1
DPM2
DPM3
EDEM1
EDEM2
EDEM3
ENGASE
FPGT
FUCA1
FUT1
FUT10
FUT11
FUT2
FUT3
FUT4
FUT5
FUT6
FUT7
FUT8
FUT9
GAL3ST2
GAL3ST3
GANAB
GCNT2
GNPNAT1
GNPTAB
GNPTG
MAN1A1
MAN1A2
MAN1B1
MAN1C1
MAN2A1
MAN2A2
MAN2B1
MAN2B2
MAN2C1
MANBA
MANEA
MGAT1
MGAT2
MGAT3
MGAT4A
MGAT4B
MGAT4C
MGAT4D
MGAT5
MOGS
NAGK
NAGPA
NEU1
NEU2
NEU3
NEU4
PRKCSH
RPN1
RPN2
ST3GAL3
ST3GAL4
ST3GAL6
ST6GAL1
ST6GAL2
ST8SIA2
ST8SIA3
ST8SIA4
STT3A
STT3B
UGGT1
UGGT2
Pathway Map
MAN1C1



General Information
Gene Symbol:   
MAN1C1
Protein name:   
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IC
Enzyme Class:   
Glycosidase
CAZy Family:   
GH47
E.C. No:   
3.2.1.113
Organs:   
Reaction (SNFG):   
MAN1C1
H2O +
→
+
Reaction (IUBMB):   
No specified reaction.
About

• Cleavage of four α1,2-mannose (α1,2-Man) residues from Man9GlcNAc2-Asn.
• ER resident Man1B1 first cleaves the single α1,2Man residue from the B branch of the Man9GlcNAc2-Asn.
• This intermediate is then rapidly cleaved in the Golgi by the concerted action of Golgi resident α-mannosidases IA, IB, and IC depending on the relative expression levels in specific cell types.
• Cleavage of Man by these enzymes is followed by the addition of GlcNAc by MGAT1.

Related Pathway
KEGG ORTHOLOGY:   
K01230
KEGG Pathway:   
ko00510  ko00513  ko01100  ko04141 
Reactome:   
R-HSA-6811438
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9NR34 
NCBI GeneID:   
57134
GlyMap GeneID:   
57134
DNA aRefSeq:   
NM_001289010.1 
Protein RefSeq:   
NP_001275939.1 
BRENDA:   
Kinetic info.
OMIM number:   
-
GeneCards database:   
MAN1C1
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Man(a1-2)Man(a1-?)
Product:  
     Man(a1-?)
Constraints:  
     nMan(a1-?)>=5&nMan(a1-?)<=7&!Man(a1-2)Man(a1-3)...Man(a1-6)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.00e-04    (pmol/cell • hr)       KM:    1.00e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-3960 27-34 -0.518 96.0 -0.518 97.0 noncon
hsa-miR-554 629-636 -0.486 99.0 -0.486 99.0 noncon
hsa-miR-8072 27-34 -0.486 96.0 -0.486 96.0 noncon
hsa-miR-1178-5p 510-517 -0.471 99.0 -0.471 99.0 noncon
hsa-miR-3147 501-508 -0.446 99.0 -0.446 99.0 noncon
hsa-miR-3196 289-295 -0.444 92.0 -0.444 93.0 noncon
hsa-miR-6816-5p 289-295 -0.436 92.0 -0.436 93.0 noncon
hsa-miR-3180-3p 289-295 -0.436 92.0 -0.436 93.0 noncon
hsa-miR-3180 289-295 -0.436 92.0 -0.436 93.0 noncon
hsa-miR-3156-3p 1963-1970 -0.401 98.0 -0.166 91.0 noncon
hsa-miR-5008-5p 568-575 -0.386 97.0 -0.386 98.0 noncon
hsa-miR-6832-5p 356-363 -0.374 97.0 -0.374 98.0 noncon
hsa-miR-885-5p 612-619 -0.372 98.0 -0.372 99.0 noncon
hsa-miR-4315 1107-1114 -0.349 93.0 -0.146 77.0 noncon
hsa-miR-5695 162-169 -0.348 98.0 -0.348 99.0 noncon
hsa-miR-6775-3p 260-267 -0.344 95.0 -0.344 96.0 noncon
hsa-miR-4504 372-379 -0.344 98.0 -0.344 99.0 noncon
hsa-miR-1291 260-267 -0.334 95.0 -0.334 96.0 noncon
hsa-miR-4731-5p 109-116 -0.328 97.0 -0.328 98.0 noncon
hsa-miR-511-3p 382-389 -0.325 98.0 -0.325 98.0 noncon
hsa-miR-6846-3p 1091-1098 -0.32 95.0 -0.134 84.0 noncon
hsa-miR-328-3p 261-268 -0.32 96.0 -0.32 97.0 con
hsa-miR-6805-5p 1315-1321 -0.314 91.0 -0.132 77.0 noncon
hsa-miR-4745-5p 242-249 -0.314 94.0 -0.314 95.0 noncon
hsa-miR-6742-5p 1938-1945 -0.304 95.0 -0.128 82.0 noncon
hsa-miR-4726-5p 202-209 -0.299 92.0 -0.299 94.0 noncon
hsa-miR-5580-5p 734-741 -0.292 94.0 -0.123 80.0 noncon
hsa-miR-3691-3p 160-167 -0.287 97.0 -0.287 97.0 noncon
hsa-miR-6779-5p 1677-1684 -0.284 94.0 -0.12 81.0 noncon
hsa-miR-181c-3p 1112-1119 -0.28 98.0 -0.119 89.0 noncon
hsa-miR-1273h-5p 1677-1684 -0.276 93.0 -0.117 80.0 noncon
hsa-miR-519a-3p 2353-2360 -0.274 96.0 -0.116 87.0 noncon
hsa-miR-519b-3p 2353-2360 -0.274 96.0 -0.116 87.0 noncon
hsa-miR-519c-3p 2353-2360 -0.274 96.0 -0.116 87.0 noncon
hsa-miR-3144-3p 751-758 -0.273 94.0 -0.116 83.0 noncon
hsa-miR-6802-3p 2242-2249 -0.27 92.0 -0.115 83.0 noncon
hsa-miR-6796-5p 1939-1946 -0.268 91.0 -0.114 77.0 noncon
hsa-miR-3689c 1677-1684 -0.268 93.0 -0.114 82.0 noncon
hsa-miR-3689b-3p 1677-1684 -0.268 93.0 -0.114 82.0 noncon
hsa-miR-3689a-3p 1677-1684 -0.268 93.0 -0.114 82.0 noncon
hsa-miR-758-3p 374-380 -0.268 96.0 -0.268 97.0 noncon
hsa-miR-30b-3p 1677-1684 -0.268 93.0 -0.114 81.0 noncon
hsa-miR-376a-3p 2278-2285 -0.262 93.0 -0.111 79.0 con
hsa-miR-924 2216-2223 -0.262 95.0 -0.111 86.0 noncon
hsa-miR-376b-3p 2278-2285 -0.262 93.0 -0.111 79.0 con
hsa-miR-206 211-217 -0.258 94.0 -0.258 95.0 con
hsa-miR-4481 242-249 -0.254 94.0 -0.254 95.0 noncon
hsa-miR-548ai 536-543 -0.252 96.0 -0.252 97.0 noncon
hsa-miR-548ba 536-543 -0.252 96.0 -0.252 97.0 noncon
hsa-miR-570-5p 536-543 -0.252 96.0 -0.252 97.0 noncon
hsa-miR-1587 916-923 -0.25 93.0 -0.107 78.0 noncon