GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ST3GAL5



General Information
Gene Symbol:   
ST3GAL5
Protein name:   
Lactosylceramide alpha-2,3-sialyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT29
E.C. No:   
2.4.99.9
Organs:   
Reaction (SNFG):   
+
→
+ CMP
+
→
+ CMP
Reaction (IUBMB):   
No specified reaction.
About

• ST3Gal5 (GM3 synthase) acts to create GM3 (starting point for ganglioside synthesis) and also GM4 (on GalCer). In its absence extended gangliosides are not formed. It may also act on GA1.
• In human tissues, expressed at high levels in brain, thymus, lung, spleen, testis, skeletal muscle, thyroid, ovary, and cervix
• ST3Gal5 competes with several enzymes to regulate the pattern of glycolipid biosynthesis. ST3Gal5 (ganglio-series) competes with β1,3-N-acetylglucosaminyltransferase (B3GNT5, lacto, neolacto), α1,3GalT (A3GALT2, isoglobo), α1,4GalT (A4GALT, golobo) and β-1,4-GalNT (B4GALNT1, ganglio) to spatially and developmentally regulated glycosphingolipids.
• GM3 contributes to insulin resistance and metabolic syndrome including type 2 diabetes.

Related Pathway
KEGG ORTHOLOGY:   
K03370
KEGG Pathway:   
ko00604  ko01100 
Reactome:   
R-HSA-4085001
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9UNP4 
NCBI GeneID:   
8869
GlyMap GeneID:   
8869
DNA aRefSeq:   
NM_001042437.1 
Protein RefSeq:   
NP_001035902.1 
BRENDA:   
Kinetic info.
OMIM number:   
604402
GeneCards database:   
ST3GAL5
Enzyme Specificity
Glycan Type:  
     GSL
Compartment:  
     
Substrate:  
     Gal(b1-?)Cer or Gal(b1-4)Glc(b1-?)Cer
Product:  
     NeuAc(a2-3)Gal(b1-?)Cer or NeuAc(a2-3)Gal(b1-4)Glc(b1-?)Cer
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    7.26e-05    (pmol/cell • hr)       KM:    2.60e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-516a-5p 566-573 -0.56 98.0 -0.56 99.0 noncon
hsa-miR-558 98-105 -0.545 99.0 -0.545 99.0 noncon
hsa-miR-6767-5p 484-491 -0.518 98.0 -0.518 98.0 noncon
hsa-miR-1324 768-775 -0.409 99.0 -0.409 99.0 noncon
hsa-miR-1205 79-86 -0.406 99.0 -0.406 99.0 noncon
hsa-miR-19b-3p 193-200 -0.4 98.0 -0.4 98.0 con
hsa-miR-19a-3p 193-200 -0.4 98.0 -0.4 98.0 con
hsa-miR-4522 812-819 -0.398 98.0 -0.398 98.0 noncon
hsa-miR-5010-3p 129-136 -0.385 99.0 -0.385 99.0 noncon
hsa-miR-6499-3p 320-327 -0.376 99.0 -0.376 99.0 noncon
hsa-miR-3918 382-389 -0.357 97.0 -0.357 98.0 noncon
hsa-miR-4326 990-996 -0.343 97.0 -0.343 98.0 noncon
hsa-miR-5681a 724-731 -0.338 98.0 -0.338 98.0 noncon
hsa-miR-520a-3p 733-740 -0.338 98.0 -0.338 98.0 con
hsa-miR-520c-3p 733-740 -0.338 98.0 -0.338 98.0 con
hsa-miR-520d-3p 733-740 -0.338 98.0 -0.338 98.0 con
hsa-miR-520b 733-740 -0.338 98.0 -0.338 98.0 con
hsa-miR-6834-3p 613-620 -0.333 96.0 -0.333 97.0 noncon
hsa-miR-372-3p 733-740 -0.33 98.0 -0.33 98.0 con
hsa-miR-520e 733-740 -0.33 98.0 -0.33 98.0 con
hsa-miR-302b-3p 733-740 -0.328 98.0 -0.328 98.0 con
hsa-miR-302c-3p.1 733-740 -0.328 98.0 -0.328 98.0 con
hsa-miR-4436b-3p 721-727 -0.325 97.0 -0.325 98.0 noncon
hsa-miR-610 143-149 -0.32 97.0 -0.32 98.0 noncon
hsa-miR-302e 733-740 -0.32 98.0 -0.32 98.0 con
hsa-miR-302d-3p 733-740 -0.32 98.0 -0.32 98.0 con
hsa-miR-302a-3p 733-740 -0.32 98.0 -0.32 98.0 con
hsa-miR-4291 555-562 -0.305 97.0 -0.305 98.0 noncon
hsa-miR-6879-5p 721-727 -0.303 96.0 -0.303 97.0 noncon
hsa-miR-6735-5p 721-727 -0.303 96.0 -0.303 97.0 noncon
hsa-miR-335-3p 968-975 -0.29 99.0 -0.29 99.0 noncon
hsa-miR-302c-3p.2 732-738 -0.278 95.0 -0.278 97.0 con
hsa-miR-520f-3p 732-738 -0.278 95.0 -0.278 97.0 con
hsa-miR-143-5p 322-329 -0.276 98.0 -0.276 98.0 noncon
hsa-miR-6795-3p 357-363 -0.276 97.0 -0.276 98.0 noncon
hsa-miR-6739-3p 792-799 -0.273 95.0 -0.273 97.0 noncon
hsa-miR-141-3p 182-188 -0.271 97.0 -0.271 98.0 con
hsa-miR-597-3p 569-576 -0.268 93.0 -0.268 95.0 noncon
hsa-miR-3127-5p 383-390 -0.266 93.0 -0.266 95.0 noncon
hsa-miR-7843-5p 721-727 -0.266 97.0 -0.266 98.0 noncon
hsa-miR-29a-5p 787-794 -0.265 97.0 -0.265 98.0 noncon
hsa-miR-2116-3p 819-826 -0.265 98.0 -0.265 98.0 noncon
hsa-miR-514a-5p 811-817 -0.265 97.0 -0.265 97.0 noncon
hsa-miR-518c-5p 810-816 -0.263 96.0 -0.263 97.0 noncon
hsa-miR-200a-3p 182-188 -0.26 97.0 -0.26 97.0 con
hsa-miR-224-5p 777-784 -0.26 97.0 -0.26 98.0 noncon
hsa-miR-373-3p 733-740 -0.26 98.0 -0.26 98.0 con
hsa-miR-6826-3p 357-363 -0.257 91.0 -0.257 94.0 noncon
hsa-miR-1254 943-949 -0.254 96.0 -0.254 97.0 noncon
hsa-miR-4257 630-637 -0.251 97.0 -0.251 98.0 noncon