GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Glycogene class
ABO
ALG1
ALG10
ALG10B
ALG11
ALG12
ALG13
ALG14
ALG1L
ALG1L2
ALG2
ALG3
ALG5
ALG6
ALG8
ALG9
B3GALNT2
B3GALT2
B3GAT1
B3GAT2
B3GNT2
B3GNT4
B3GNT7
B3GNT8
B4GALNT2
B4GALNT3
B4GALNT4
B4GALT1
B4GALT2
B4GALT3
B4GALT4
B4GALT5
CHST1
CHST10
CHST2
CHST3
CHST4
CHST5
CHST8
CHST9
CMAS
CTBS
DAD1
DDOST
DPAGT1
DPM1
DPM2
DPM3
EDEM1
EDEM2
EDEM3
ENGASE
FPGT
FUCA1
FUT1
FUT10
FUT11
FUT2
FUT3
FUT4
FUT5
FUT6
FUT7
FUT8
FUT9
GAL3ST2
GAL3ST3
GANAB
GCNT2
GNPNAT1
GNPTAB
GNPTG
MAN1A1
MAN1A2
MAN1B1
MAN1C1
MAN2A1
MAN2A2
MAN2B1
MAN2B2
MAN2C1
MANBA
MANEA
MGAT1
MGAT2
MGAT3
MGAT4A
MGAT4B
MGAT4C
MGAT4D
MGAT5
MOGS
NAGK
NAGPA
NEU1
NEU2
NEU3
NEU4
PRKCSH
RPN1
RPN2
ST3GAL3
ST3GAL4
ST3GAL6
ST6GAL1
ST6GAL2
ST8SIA2
ST8SIA3
ST8SIA4
STT3A
STT3B
UGGT1
UGGT2
Pathway Map
MAN1A1



General Information
Gene Symbol:   
MAN1A1
Protein name:   
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA
Enzyme Class:   
Glycosidase
CAZy Family:   
GH47
E.C. No:   
3.2.1.113
Organs:   
Reaction (SNFG):   
MAN1A1
H2O +
→
+
Reaction (IUBMB):   
No specified reaction.
About

• Cleavage of four α1,2-mannose (α1,2-Man) residues from Man9GlcNAc2-Asn.
• ER resident Man1B1 first cleaves the single α1,2Man residue from the B branch of the Man9GlcNAc2-Asn.
• This intermediate is then rapidly cleaved in the Golgi by the concerted action of Golgi resident α-mannosidases IA, IB, and IC depending on the relative expression levels in specific cell types.
• Cleavage of Man by these enzymes is followed by the addition of GlcNAc by MGAT1.

Related Pathway
KEGG ORTHOLOGY:   
K01230
KEGG Pathway:   
ko00510  ko00513  ko01100  ko04141 
Reactome:   
R-HSA-6811438
Related Database
Organism:   
Homo sapiens
Uniprot:   
P33908 
NCBI GeneID:   
4121
GlyMap GeneID:   
4121
DNA aRefSeq:   
NM_005907.3 
Protein RefSeq:   
NP_005898.2 
BRENDA:   
Kinetic info.
OMIM number:   
604344
GeneCards database:   
MAN1A1
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Man(a1-2)Man(a1-?)
Product:  
     Man(a1-?)
Constraints:  
     nMan(a1-?)>=5&nMan(a1-?)<=7&!Man(a1-2)Man(a1-3)...Man(a1-6)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.00e-04    (pmol/cell • hr)       KM:    1.00e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 80 60 40 20 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4784 2611-2618 -0.537 99.0 0.0 0.0 noncon
hsa-miR-6081 22-29 -0.53 99.0 -0.53 99.0 noncon
hsa-miR-3150b-3p 2611-2618 -0.529 99.0 0.0 0.0 noncon
hsa-miR-6802-5p 1530-1537 -0.436 97.0 -0.293 94.0 noncon
hsa-miR-455-3p.2 331-338 -0.431 99.0 -0.431 99.0 con
hsa-miR-4638-5p 1426-1433 -0.43 95.0 -0.289 92.0 noncon
hsa-miR-7515 31-38 -0.402 98.0 -0.402 99.0 noncon
hsa-miR-135b-5p 405-412 -0.401 98.0 -0.401 98.0 con
hsa-miR-135a-5p 405-412 -0.401 98.0 -0.401 98.0 con
hsa-miR-550a-3-5p 71-78 -0.393 97.0 -0.393 98.0 noncon
hsa-miR-550a-5p 71-78 -0.393 97.0 -0.393 98.0 noncon
hsa-miR-1271-3p 71-78 -0.393 97.0 -0.393 98.0 noncon
hsa-miR-620 2517-2524 -0.391 99.0 -0.252 97.0 noncon
hsa-miR-365a-5p 2139-2146 -0.384 98.0 -0.256 96.0 noncon
hsa-miR-3165 205-212 -0.382 98.0 -0.382 99.0 noncon
hsa-miR-4745-5p 1786-1793 -0.376 97.0 -0.255 92.0 noncon
hsa-miR-129-1-3p 1370-1377 -0.372 98.0 -0.252 96.0 noncon
hsa-miR-4689 2612-2618 -0.363 98.0 0.0 0.0 noncon
hsa-miR-365b-5p 2139-2146 -0.36 98.0 -0.241 95.0 noncon
hsa-miR-129-2-3p 1370-1377 -0.356 98.0 -0.242 96.0 noncon
hsa-miR-1270 2517-2524 -0.354 98.0 -0.229 97.0 noncon
hsa-miR-1296-3p 742-749 -0.35 98.0 -0.35 98.0 noncon
hsa-miR-4481 1786-1793 -0.34 97.0 -0.231 94.0 noncon
hsa-miR-182-5p 1875-1882 -0.331 96.0 -0.225 92.0 noncon
hsa-miR-3913-5p 2141-2148 -0.321 98.0 -0.216 97.0 noncon
hsa-miR-3613-5p 820-826 -0.315 96.0 -0.315 97.0 noncon
hsa-miR-4424 1931-1938 -0.312 96.0 -0.21 92.0 noncon
hsa-miR-6858-5p 2612-2618 -0.312 98.0 0.0 0.0 noncon
hsa-miR-1197 673-679 -0.311 98.0 -0.311 98.0 noncon
hsa-miR-6504-5p 195-201 -0.311 97.0 -0.311 98.0 noncon
hsa-miR-8071 469-476 -0.307 96.0 -0.307 97.0 noncon
hsa-miR-520b 2324-2331 -0.304 97.0 -0.198 94.0 con
hsa-miR-520c-3p 2324-2331 -0.304 97.0 -0.198 94.0 con
hsa-miR-499a-3p 2188-2195 -0.303 98.0 -0.204 96.0 noncon
hsa-miR-499b-3p 2188-2195 -0.303 98.0 -0.204 96.0 noncon
hsa-miR-6757-3p 198-205 -0.296 98.0 -0.296 98.0 noncon
hsa-miR-4418 621-628 -0.295 98.0 -0.295 98.0 noncon
hsa-miR-520a-5p 246-252 -0.292 96.0 -0.292 97.0 noncon
hsa-miR-6886-3p 1394-1401 -0.291 93.0 -0.199 90.0 noncon
hsa-miR-4791 178-184 -0.288 95.0 -0.288 96.0 noncon
hsa-miR-520e 2324-2331 -0.288 97.0 -0.188 94.0 con
hsa-miR-4468 21-27 -0.283 96.0 -0.283 97.0 noncon
hsa-miR-509-3-5p 621-628 -0.282 98.0 -0.282 98.0 noncon
hsa-miR-509-5p 621-628 -0.282 98.0 -0.282 98.0 noncon
hsa-miR-520a-3p 2324-2331 -0.28 97.0 -0.183 94.0 con
hsa-miR-520d-3p 2324-2331 -0.28 97.0 -0.183 94.0 con
hsa-miR-216b-5p 2172-2179 -0.276 97.0 -0.186 95.0 noncon
hsa-miR-4256 1436-1443 -0.272 94.0 -0.186 91.0 noncon
hsa-miR-4456 207-213 -0.272 94.0 -0.272 96.0 noncon
hsa-miR-6864-5p 32-38 -0.271 95.0 -0.271 96.0 noncon
hsa-miR-525-5p 246-252 -0.27 96.0 -0.27 97.0 noncon
hsa-miR-942-3p 392-398 -0.268 95.0 -0.268 97.0 noncon
hsa-miR-744-3p 2089-2096 -0.265 94.0 -0.179 91.0 noncon
hsa-miR-372-3p 2324-2331 -0.264 96.0 -0.173 93.0 con
hsa-miR-6888-5p 2610-2616 -0.263 98.0 0.0 0.0 noncon
hsa-miR-6716-5p 28-34 -0.262 93.0 -0.262 95.0 noncon
hsa-miR-5088-5p 1831-1837 -0.258 94.0 -0.177 91.0 noncon
hsa-miR-3064-5p 195-201 -0.256 95.0 -0.256 96.0 noncon
hsa-miR-1915-5p 1398-1405 -0.256 90.0 -0.176 87.0 noncon
hsa-miR-26a-2-3p 1285-1292 -0.252 95.0 -0.173 93.0 noncon
hsa-miR-26a-1-3p 1285-1292 -0.252 95.0 -0.173 93.0 noncon
hsa-miR-4276 790-797 -0.25 94.0 -0.25 96.0 noncon