GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
NAGA



General Information
Gene Symbol:   
NAGA
Protein name:   
Alpha-N-acetylgalactosaminidase
Enzyme Class:   
Glycosidase
CAZy Family:   
GH27
E.C. No:   
3.2.1.49
Tissues:   
all, Muscular, Epithelial, Connective, Nervous
Organs:   
Reaction (SNFG):   
NAGA
H2O +
→
+
Reaction (IUBMB):   
No specified reaction.
About

• N-acetylgalactosaminidase hydrolyzes GalNAc from glycosphingolipids.
• Schindler disease is caused by the lack of NAGA activity

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
No Record
Related Database
Organism:   
Homo sapiens
Uniprot:   
P17050 
NCBI GeneID:   
4668
GlyMap GeneID:   
4668
DNA aRefSeq:   
NM_000262.2 
Protein RefSeq:   
NP_000253.1 
BRENDA:   
Kinetic info.
OMIM number:   
104170
GeneCards database:   
NAGA
Enzyme Specificity
Glycan Type:  
     GSL
Compartment:  
     
Substrate:  
     GalNAc(b1-4)...Gal()
Product:  
     Gal()
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-635 1615-1622 -0.459 98.0 -0.352 98.0 noncon
hsa-miR-5699-5p 193-200 -0.455 99.0 -0.455 99.0 noncon
hsa-miR-1587 1613-1620 -0.446 99.0 -0.342 98.0 noncon
hsa-miR-6774-5p 1615-1622 -0.435 98.0 -0.334 97.0 noncon
hsa-miR-4656 1612-1619 -0.432 99.0 -0.332 98.0 noncon
hsa-miR-193a-3p 1758-1764 -0.416 97.0 -0.32 96.0 noncon
hsa-miR-5589-5p 1900-1907 -0.416 99.0 -0.281 99.0 noncon
hsa-miR-193b-3p 1758-1764 -0.416 97.0 -0.32 96.0 noncon
hsa-miR-3690 929-936 -0.404 98.0 -0.311 98.0 noncon
hsa-miR-3154 1745-1752 -0.395 99.0 -0.304 98.0 noncon
hsa-miR-3138 916-923 -0.386 96.0 -0.297 94.0 noncon
hsa-miR-433-3p 1848-1855 -0.381 98.0 -0.289 98.0 noncon
hsa-miR-7156-3p 885-892 -0.371 97.0 -0.286 96.0 noncon
hsa-miR-3620-5p 1613-1620 -0.37 98.0 -0.285 98.0 noncon
hsa-miR-939-5p 90-97 -0.367 97.0 -0.367 97.0 noncon
hsa-miR-3200-3p 1815-1822 -0.364 98.0 -0.276 97.0 noncon
hsa-miR-197-5p 414-421 -0.354 93.0 -0.354 95.0 noncon
hsa-miR-1343-5p 90-97 -0.351 97.0 -0.351 97.0 noncon
hsa-miR-4788 1031-1037 -0.349 92.0 -0.27 90.0 noncon
hsa-miR-3132 414-421 -0.341 93.0 -0.341 95.0 noncon
hsa-miR-645 39-46 -0.337 95.0 -0.337 97.0 noncon
hsa-miR-601 42-49 -0.336 94.0 -0.336 95.0 noncon
hsa-miR-5579-5p 1932-1938 -0.325 97.0 0.0 0.0 noncon
hsa-miR-6841-5p 1820-1826 -0.316 94.0 -0.241 93.0 noncon
hsa-miR-4667-5p 906-913 -0.31 96.0 -0.24 94.0 noncon
hsa-miR-6731-5p 873-880 -0.31 95.0 -0.24 93.0 noncon
hsa-miR-3922-3p 1757-1763 -0.306 97.0 -0.237 96.0 noncon
hsa-miR-8089 906-913 -0.305 96.0 -0.236 94.0 noncon
hsa-miR-8073 379-386 -0.305 98.0 -0.305 98.0 noncon
hsa-miR-221-5p 379-386 -0.305 98.0 -0.305 98.0 noncon
hsa-miR-943 986-993 -0.304 97.0 -0.236 96.0 noncon
hsa-miR-6794-3p 1883-1890 -0.304 96.0 -0.208 94.0 noncon
hsa-miR-6882-5p 1802-1808 -0.301 97.0 -0.234 97.0 noncon
hsa-miR-8069 1920-1927 -0.297 97.0 0.0 0.0 noncon
hsa-miR-3176 1757-1763 -0.296 97.0 -0.23 96.0 noncon
hsa-miR-6861-3p 1242-1248 -0.295 92.0 -0.229 91.0 noncon
hsa-miR-8085 873-880 -0.294 95.0 -0.228 92.0 noncon
hsa-miR-150-3p 1931-1937 -0.29 91.0 0.0 0.0 noncon
hsa-miR-6862-5p 285-292 -0.288 90.0 -0.288 93.0 noncon
hsa-miR-1911-3p 707-714 -0.286 94.0 -0.222 93.0 noncon
hsa-miR-6864-5p 1696-1702 -0.285 96.0 -0.221 94.0 noncon
hsa-miR-4700-5p 906-913 -0.284 96.0 -0.221 94.0 noncon
hsa-miR-4520-3p 160-167 -0.283 96.0 -0.283 97.0 noncon
hsa-miR-7973 1335-1341 -0.281 92.0 -0.218 91.0 noncon
hsa-miR-4742-3p 1796-1803 -0.28 96.0 -0.218 96.0 noncon
hsa-miR-4731-5p 1901-1907 -0.278 96.0 -0.191 91.0 noncon
hsa-miR-6730-5p 1607-1614 -0.276 98.0 -0.214 97.0 noncon
hsa-miR-6878-3p 745-752 -0.272 94.0 -0.212 93.0 noncon
hsa-miR-3151-5p 242-249 -0.271 95.0 -0.271 96.0 noncon
hsa-miR-6783-3p 1730-1736 -0.271 95.0 -0.211 94.0 noncon
hsa-miR-1260b 1491-1498 -0.268 93.0 -0.208 92.0 noncon
hsa-miR-1343-3p 1730-1736 -0.268 95.0 -0.208 94.0 noncon
hsa-miR-1260a 1491-1498 -0.268 93.0 -0.208 92.0 noncon
hsa-miR-214-5p 16-22 -0.266 96.0 -0.266 97.0 con
hsa-miR-188-3p 1529-1536 -0.265 92.0 -0.206 90.0 noncon
hsa-miR-4325 130-137 -0.264 96.0 -0.264 97.0 noncon
hsa-miR-661 1412-1418 -0.259 95.0 -0.202 93.0 noncon
hsa-miR-3192-5p 1728-1734 -0.258 97.0 -0.201 96.0 noncon
hsa-miR-4316 678-685 -0.256 95.0 -0.199 94.0 noncon
hsa-miR-5588-5p 667-674 -0.256 96.0 -0.199 95.0 noncon
hsa-miR-6500-5p 1512-1519 -0.255 94.0 -0.199 92.0 noncon
hsa-miR-4487 1909-1915 -0.254 93.0 -0.003 28.0 noncon
hsa-miR-4802-5p 1891-1897 -0.253 94.0 -0.174 91.0 noncon
hsa-miR-3120-3p 1640-1647 -0.253 97.0 -0.197 96.0 noncon
hsa-miR-6871-5p 1220-1227 -0.252 93.0 -0.196 92.0 noncon