GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ST6GALNAC6



General Information
Gene Symbol:   
ST6GALNAC6
Protein name:   
Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT29
E.C. No:   
2.4.99.7
Organs:   
Reaction (SNFG):   
+
→
+ CMP
Reaction (IUBMB):   
No specified reaction.
About

• ST6GALNAC6 synthesizes GD1a, GT1aa, and GQ1ba from GM1b, GD1a, and GT1b, respectively. Thus, this enzyme creates disialyl-Lewis a structure using sialyl-Lc4. It acts on lacto-series and globo-series substrates.
• Ubiquitously expressed in many organs, and associated with cancer. Upregulation of ST6GALNAC6 was reported in all livers of tumor-bearing mice of Engelbreth-Holm Swarm sarcoma.

Related Pathway
KEGG ORTHOLOGY:   
K03376
KEGG Pathway:   
ko00604  ko01100 
Reactome:   
R-HSA-4085001
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q969X2 
NCBI GeneID:   
30815
GlyMap GeneID:   
30815
DNA aRefSeq:   
NM_001286999.1 
Protein RefSeq:   
NP_001273928.1 
BRENDA:   
Kinetic info.
OMIM number:   
610135
GeneCards database:   
ST6GALNAC6
Enzyme Specificity
Glycan Type:  
     GSL
Compartment:  
     
Substrate:  
     NeuAc(a2-3)Gal(b1-3)GalNAc(b1-?)
Product:  
     NeuAc(a2-3)Gal(b1-3)[NeuAc(a2-6)]GalNAc(b1-?)
Constraints:  
     nNeuAc(a2-?)>=1&nNeuAc(a2-?)<=3
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 60 50 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-let-7i-3p 774-781 -0.719 99.0 -0.719 99.0 noncon
hsa-miR-4640-3p 258-265 -0.561 99.0 -0.561 99.0 noncon
hsa-miR-933 236-243 -0.56 97.0 -0.56 98.0 noncon
hsa-miR-7109-5p 337-344 -0.553 98.0 -0.553 99.0 noncon
hsa-miR-486-5p 1250-1257 -0.515 99.0 -0.323 98.0 con
hsa-miR-5008-5p 1076-1083 -0.514 99.0 -0.514 99.0 noncon
hsa-miR-4505 107-114 -0.507 99.0 -0.507 99.0 noncon
hsa-miR-3184-5p 842-849 -0.501 98.0 -0.501 99.0 noncon
hsa-miR-423-5p 842-849 -0.493 98.0 -0.493 99.0 noncon
hsa-miR-1203 1272-1278 -0.446 96.0 -0.159 77.0 noncon
hsa-miR-6852-5p 324-331 -0.429 99.0 -0.429 99.0 noncon
hsa-miR-6798-3p 259-265 -0.428 96.0 -0.428 97.0 noncon
hsa-miR-5787 107-114 -0.405 99.0 -0.405 99.0 noncon
hsa-miR-4435 78-85 -0.402 98.0 -0.402 98.0 noncon
hsa-miR-665 750-757 -0.37 98.0 -0.37 98.0 noncon
hsa-miR-4505 688-695 -0.369 97.0 -0.369 98.0 noncon
hsa-miR-544b 19-26 -0.363 98.0 -0.363 99.0 noncon
hsa-miR-6842-3p 108-115 -0.36 98.0 -0.36 98.0 noncon
hsa-miR-17-5p 1060-1067 -0.357 98.0 -0.357 99.0 con
hsa-miR-106a-5p 1060-1067 -0.354 98.0 -0.354 99.0 con
hsa-miR-4750-3p 27-34 -0.351 97.0 -0.351 98.0 noncon
hsa-miR-4283 321-328 -0.35 96.0 -0.35 96.0 noncon
hsa-miR-6851-3p 876-883 -0.345 97.0 -0.345 98.0 noncon
hsa-miR-6734-5p 407-414 -0.342 96.0 -0.342 97.0 noncon
hsa-miR-519d-3p 1060-1067 -0.341 98.0 -0.341 99.0 con
hsa-miR-20b-5p 1060-1067 -0.341 98.0 -0.341 99.0 con
hsa-miR-4646-3p 815-822 -0.34 96.0 -0.34 97.0 noncon
hsa-miR-135b-3p 646-653 -0.338 94.0 -0.338 95.0 noncon
hsa-miR-1271-3p 1057-1063 -0.335 95.0 -0.335 97.0 noncon
hsa-miR-668-5p 928-934 -0.334 91.0 -0.334 93.0 noncon
hsa-miR-93-5p 1060-1067 -0.333 98.0 -0.333 99.0 con
hsa-miR-106b-5p 1060-1067 -0.328 98.0 -0.328 99.0 con
hsa-miR-6798-3p 443-449 -0.321 90.0 -0.321 94.0 noncon
hsa-miR-20a-5p 1060-1067 -0.32 98.0 -0.32 99.0 con
hsa-miR-548q 421-428 -0.304 98.0 -0.304 98.0 noncon
hsa-miR-550a-5p 1057-1063 -0.302 94.0 -0.302 96.0 noncon
hsa-miR-550a-3-5p 1057-1063 -0.302 94.0 -0.302 96.0 noncon
hsa-miR-7152-3p 1158-1165 -0.301 95.0 -0.194 91.0 noncon
hsa-miR-6775-3p 117-123 -0.301 93.0 -0.301 94.0 noncon
hsa-miR-4489 840-846 -0.296 96.0 -0.296 97.0 noncon
hsa-miR-519e-3p 1058-1064 -0.292 94.0 -0.292 96.0 noncon
hsa-miR-1291 117-123 -0.289 93.0 -0.289 94.0 noncon
hsa-miR-3650 1262-1268 -0.287 95.0 -0.185 92.0 noncon
hsa-miR-6085 841-847 -0.284 95.0 -0.284 96.0 noncon
hsa-miR-3922-5p 120-127 -0.28 95.0 -0.28 97.0 noncon
hsa-miR-2355-5p 157-164 -0.279 96.0 -0.279 97.0 noncon
hsa-miR-33b-3p 1058-1064 -0.277 94.0 -0.277 96.0 noncon
hsa-miR-5787 688-695 -0.275 96.0 -0.275 97.0 noncon
hsa-miR-3652 141-148 -0.274 97.0 -0.274 97.0 noncon
hsa-miR-4319 177-183 -0.274 94.0 -0.274 96.0 con
hsa-miR-6775-3p 151-157 -0.273 91.0 -0.273 93.0 noncon
hsa-miR-524-3p 931-937 -0.273 92.0 -0.273 94.0 noncon
hsa-miR-4430 141-148 -0.271 97.0 -0.271 97.0 noncon
hsa-miR-3943 966-972 -0.269 93.0 -0.269 95.0 noncon
hsa-miR-6813-5p 841-847 -0.269 94.0 -0.269 96.0 noncon
hsa-miR-1266-5p 555-562 -0.263 96.0 -0.263 97.0 noncon
hsa-miR-1291 151-157 -0.261 90.0 -0.261 93.0 noncon
hsa-miR-526b-3p 1060-1067 -0.26 98.0 -0.26 98.0 con
hsa-miR-6763-5p 348-354 -0.256 93.0 -0.256 94.0 noncon
hsa-miR-6842-3p 142-149 -0.255 93.0 -0.255 95.0 noncon
hsa-miR-6802-3p 1198-1204 -0.252 90.0 -0.163 87.0 noncon
hsa-miR-5193 126-133 -0.252 92.0 -0.252 93.0 noncon
hsa-miR-125b-5p 177-183 -0.25 93.0 -0.25 95.0 con