GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Glycogene class
A3GALT2
A4GALT
A4GNT
ABO
B3GALNT1
B3GALNT2
B3GALT1
B3GALT2
B3GALT4
B3GALT5
B3GAT1
B3GAT2
B3GNT2
B3GNT3
B3GNT4
B3GNT5
B3GNT6
B3GNT7
B4GALNT1
B4GALNT2
B4GALNT3
B4GALNT4
B4GALT1
B4GALT2
B4GALT3
B4GALT4
B4GALT5
B4GALT6
C1GALT1
C1GALT1C1
CHST1
CHST10
CHST2
CHST3
CHST4
CHST5
CHST8
CHST9
CMAS
FPGT
FUT1
FUT2
FUT3
FUT4
FUT5
FUT6
FUT7
FUT9
GAL3ST1
GAL3ST2
GAL3ST3
GAL3ST4
GALNT1
GALNT10
GALNT11
GALNT12
GALNT13
GALNT14
GALNT15
GALNT16
GALNT17
GALNT18
GALNT2
GALNT3
GALNT4
GALNT5
GALNT6
GALNT7
GALNT8
GALNT9
GALNTL6
GBGT1
GCNT1
GCNT2
GCNT3
GCNT4
HEXA
HEXB
NAGA
NEU1
NEU2
NEU3
NEU4
ST3GAL1
ST3GAL2
ST3GAL3
ST3GAL4
ST3GAL5
ST3GAL6
ST6GAL1
ST6GAL2
ST6GALNAC1
ST6GALNAC2
ST6GALNAC3
ST6GALNAC4
ST6GALNAC5
ST6GALNAC6
ST8SIA1
ST8SIA2
ST8SIA3
ST8SIA4
ST8SIA5
ST8SIA6
UGCG
UGT8
Pathway Map
B3GNT3



General Information
Gene Symbol:   
B3GNT3
Protein name:   
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT31
E.C. No:   
2.4.1.149
Organs:   
Reaction (SNFG):   
+
→
+ UDP
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• This is the core 1 extension β1,3GnT enzyme. A Golgi localized Type-2 GlycoT.
• Core-1 extension is possible even after core-2 branching.
• The enzyme exhibits no activity for Type 2 and Type 1 LacNAc chains, thus it is O-glycan specific.
• 6-sulfo extended core-1 structure formed by this enzyme along with the sufotransferase GlcNAc6ST-2/CHST4. This extended epitope is called the 'MECA-79 epitope' which is commonly expressed on HEV and secondary lymphoid organs.
• MECA-79 binds lymphocyte L-selectin, and is thus critical for lymphocyte homing.
• Additionally, the enzyme can support the formation of 6'sulfo sLeX type glycans on the core-1 chain.
• Other organs expressing this enzyme include the mucin producing tissue: small and large intestines, stomach, esophagus, trachea and bladder.

Related Pathway
KEGG ORTHOLOGY:   
K07970
KEGG Pathway:   
ko00601  ko01100 
Reactome:   
R-HSA-2022854
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9Y2A9 
NCBI GeneID:   
10331
GlyMap GeneID:   
10331
DNA aRefSeq:   
NM_014256.3 
Protein RefSeq:   
NP_055071.2 
BRENDA:   
Kinetic info.
OMIM number:   
605863
GeneCards database:   
B3GNT3
Enzyme Specificity
Glycan Type:  
     O-linked (mucin type), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-3)...GalNAc(a1-?)Ser|Thr or Gal(b1-?)GlcNAc(b1-?)
Product:  
     GlcNAc(b1-3)Gal(b1-3)...GalNAc(a1-?)Ser|Thr or GlcNAc(b1-3)Gal(b1-?)GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.70e-06    (pmol/cell • hr)       KM:    7.00e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6742-5p 1446-1453 -0.725 99.0 -0.001 17.0 noncon
hsa-miR-4632-3p 1303-1310 -0.716 99.0 -0.001 8.0 noncon
hsa-miR-3691-3p 953-960 -0.587 99.0 -0.587 99.0 noncon
hsa-miR-6796-5p 1447-1453 -0.509 98.0 -0.001 23.0 noncon
hsa-miR-6773-5p 734-741 -0.501 99.0 -0.501 99.0 noncon
hsa-miR-6756-5p 1390-1397 -0.477 98.0 -0.001 18.0 noncon
hsa-miR-6724-5p 734-741 -0.474 98.0 -0.474 99.0 noncon
hsa-miR-3160-3p 1436-1443 -0.473 99.0 -0.001 19.0 noncon
hsa-miR-6766-5p 1390-1397 -0.472 98.0 -0.001 22.0 noncon
hsa-miR-6757-5p 1015-1022 -0.467 99.0 -0.001 24.0 noncon
hsa-miR-3189-5p 918-925 -0.45 99.0 -0.45 99.0 noncon
hsa-miR-6848-5p 670-677 -0.424 95.0 -0.424 96.0 noncon
hsa-miR-6846-5p 670-677 -0.416 95.0 -0.416 96.0 noncon
hsa-miR-6811-3p 905-912 -0.414 98.0 -0.414 99.0 noncon
hsa-miR-6778-3p 611-618 -0.401 98.0 -0.401 99.0 noncon
hsa-miR-556-5p 1438-1445 -0.392 99.0 -0.001 37.0 noncon
hsa-miR-6846-5p 865-872 -0.391 94.0 -0.391 96.0 noncon
hsa-miR-3665 362-369 -0.389 97.0 -0.389 98.0 noncon
hsa-miR-6836-3p 612-619 -0.381 98.0 -0.381 98.0 noncon
hsa-miR-4524a-5p 245-252 -0.381 96.0 -0.381 98.0 noncon
hsa-miR-6740-3p 1314-1321 -0.376 97.0 -0.001 21.0 noncon
hsa-miR-4524b-5p 245-252 -0.373 96.0 -0.373 97.0 noncon
hsa-miR-6848-5p 865-872 -0.367 93.0 -0.367 95.0 noncon
hsa-miR-4764-5p 157-163 -0.366 97.0 -0.366 97.0 noncon
hsa-miR-3152-5p 1345-1352 -0.365 97.0 -0.001 28.0 noncon
hsa-miR-1267 995-1002 -0.362 98.0 -0.001 24.0 noncon
hsa-miR-491-5p 1447-1453 -0.36 97.0 -0.001 11.0 noncon
hsa-miR-6856-3p 1085-1092 -0.353 97.0 -0.001 30.0 noncon
hsa-miR-516b-5p 216-223 -0.351 97.0 -0.351 98.0 noncon
hsa-miR-6722-3p 1399-1405 -0.345 96.0 -0.001 24.0 noncon
hsa-miR-4727-5p 253-260 -0.342 97.0 -0.342 98.0 noncon
hsa-miR-6769b-5p 170-176 -0.34 97.0 -0.34 98.0 noncon
hsa-miR-7154-3p 791-798 -0.339 98.0 -0.339 98.0 noncon
hsa-miR-299-3p 1448-1454 -0.332 97.0 -0.001 26.0 noncon
hsa-miR-6769a-5p 170-176 -0.33 97.0 -0.33 98.0 noncon
hsa-miR-708-5p 1421-1427 -0.328 97.0 -0.001 22.0 noncon
hsa-miR-6078 1179-1185 -0.327 96.0 -0.001 11.0 noncon
hsa-miR-3663-3p 1190-1197 -0.327 94.0 -0.001 22.0 noncon
hsa-miR-3139 1421-1427 -0.327 97.0 -0.001 22.0 noncon
hsa-miR-939-3p 1134-1141 -0.326 97.0 -0.001 41.0 noncon
hsa-miR-3138 426-433 -0.322 92.0 -0.322 95.0 noncon
hsa-miR-7113-5p 196-203 -0.318 97.0 -0.318 98.0 noncon
hsa-miR-6855-5p 1445-1451 -0.318 96.0 -0.001 20.0 noncon
hsa-miR-28-5p 1421-1427 -0.317 97.0 -0.001 21.0 noncon
hsa-miR-3170 1445-1451 -0.315 96.0 -0.001 24.0 noncon
hsa-miR-4667-3p 607-614 -0.311 97.0 -0.311 97.0 noncon
hsa-miR-6756-5p 1394-1400 -0.31 91.0 -0.001 17.0 noncon
hsa-miR-4645-5p 767-774 -0.309 98.0 -0.309 98.0 noncon
hsa-miR-4633-3p 1420-1426 -0.308 97.0 -0.001 14.0 noncon
hsa-miR-5695 955-962 -0.308 98.0 -0.001 32.0 noncon
hsa-miR-1913 920-926 -0.307 96.0 -0.307 97.0 noncon
hsa-miR-5187-5p 1014-1020 -0.305 95.0 -0.001 14.0 noncon
hsa-miR-6798-5p 1398-1404 -0.299 95.0 -0.001 25.0 noncon
hsa-miR-4419a 804-811 -0.298 95.0 -0.298 96.0 noncon
hsa-miR-6127 804-811 -0.298 94.0 -0.298 96.0 noncon
hsa-miR-1909-3p 1399-1405 -0.298 94.0 -0.001 25.0 noncon
hsa-miR-324-3p 920-926 -0.297 96.0 -0.297 97.0 noncon
hsa-miR-6817-5p 378-385 -0.296 97.0 -0.296 98.0 noncon
hsa-miR-4769-3p 378-385 -0.296 97.0 -0.296 98.0 noncon
hsa-miR-6817-5p 252-259 -0.294 97.0 -0.294 98.0 noncon
hsa-miR-4673 767-774 -0.291 98.0 -0.291 98.0 noncon
hsa-miR-939-3p 461-468 -0.291 96.0 -0.291 97.0 noncon
hsa-miR-6133 804-811 -0.29 94.0 -0.29 95.0 noncon
hsa-miR-1343-3p 63-70 -0.283 96.0 -0.283 97.0 noncon
hsa-miR-4656 458-465 -0.282 96.0 -0.282 97.0 noncon
hsa-miR-4447 1447-1453 -0.282 96.0 -0.001 30.0 noncon
hsa-miR-4472 1447-1453 -0.282 96.0 -0.001 31.0 noncon
hsa-miR-4677-3p 337-344 -0.28 97.0 -0.28 98.0 noncon
hsa-miR-4437 1131-1138 -0.28 92.0 -0.001 12.0 noncon
hsa-miR-4769-3p 252-259 -0.278 97.0 -0.278 98.0 noncon
hsa-miR-6734-3p 108-114 -0.278 95.0 -0.278 96.0 noncon
hsa-miR-6745 1391-1397 -0.276 94.0 -0.001 12.0 noncon
hsa-miR-4691-3p 242-248 -0.275 96.0 -0.275 97.0 noncon
hsa-miR-6500-5p 1420-1426 -0.275 96.0 -0.001 13.0 noncon
hsa-miR-4510 804-811 -0.274 93.0 -0.274 95.0 noncon
hsa-miR-6130 804-811 -0.274 93.0 -0.274 95.0 noncon
hsa-miR-5699-5p 919-925 -0.274 96.0 -0.274 97.0 noncon
hsa-miR-6129 804-811 -0.274 93.0 -0.274 95.0 noncon
hsa-miR-4751 657-664 -0.272 93.0 -0.272 95.0 noncon
hsa-miR-6864-5p 1413-1419 -0.271 95.0 -0.001 29.0 noncon
hsa-miR-3194-5p 141-147 -0.271 94.0 -0.271 96.0 noncon
hsa-miR-6512-3p 715-722 -0.27 95.0 -0.27 97.0 noncon
hsa-miR-6720-5p 715-722 -0.27 95.0 -0.27 97.0 noncon
hsa-miR-2113 1160-1167 -0.269 97.0 -0.001 45.0 noncon
hsa-miR-6776-5p 810-816 -0.266 95.0 -0.266 96.0 noncon
hsa-miR-6868-5p 890-896 -0.263 95.0 -0.263 96.0 noncon
hsa-miR-4283 669-675 -0.261 91.0 -0.261 93.0 noncon
hsa-miR-4751 790-796 -0.259 92.0 -0.259 94.0 noncon
hsa-miR-939-3p 1251-1258 -0.258 95.0 -0.001 40.0 noncon
hsa-miR-5187-5p 1386-1393 -0.257 92.0 -0.001 14.0 noncon
hsa-miR-3064-5p 410-417 -0.254 95.0 -0.254 96.0 noncon
hsa-miR-6783-3p 63-70 -0.254 95.0 -0.254 96.0 noncon
hsa-miR-6504-5p 410-417 -0.254 95.0 -0.254 96.0 noncon
hsa-miR-3134 227-233 -0.254 95.0 -0.254 96.0 noncon
hsa-miR-1306-5p 593-599 -0.253 94.0 -0.253 96.0 noncon
hsa-miR-6510-5p 322-328 -0.252 94.0 -0.252 96.0 noncon
hsa-miR-199a-5p 1050-1056 -0.251 93.0 -0.001 32.0 noncon
hsa-miR-4487 1437-1443 -0.25 93.0 -0.001 22.0 noncon