GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Glycogene class
A3GALT2
A4GALT
A4GNT
ABO
ALG1
ALG10
ALG10B
ALG11
ALG12
ALG13
ALG14
ALG1L
ALG1L2
ALG2
ALG3
ALG5
ALG6
ALG8
ALG9
B3GALNT1
B3GALNT2
B3GALT1
B3GALT2
B3GALT4
B3GALT5
B3GAT1
B3GAT2
B3GNT2
B3GNT3
B3GNT4
B3GNT5
B3GNT6
B3GNT7
B3GNT8
B4GALNT1
B4GALNT2
B4GALNT3
B4GALNT4
B4GALT1
B4GALT2
B4GALT3
B4GALT4
B4GALT5
B4GALT6
C1GALT1
C1GALT1C1
CHST1
CHST10
CHST2
CHST3
CHST4
CHST5
CHST8
CHST9
CMAS
CTBS
DAD1
DDOST
DPAGT1
DPM1
DPM2
DPM3
EDEM1
EDEM2
EDEM3
ENGASE
FPGT
FUCA1
FUT1
FUT10
FUT11
FUT2
FUT3
FUT4
FUT5
FUT6
FUT7
FUT8
FUT9
GAL3ST1
GAL3ST2
GAL3ST3
GAL3ST4
GALNT1
GALNT10
GALNT11
GALNT12
GALNT13
GALNT14
GALNT15
GALNT16
GALNT17
GALNT18
GALNT2
GALNT3
GALNT4
GALNT5
GALNT6
GALNT7
GALNT8
GALNT9
GALNTL6
GANAB
GBGT1
GCNT1
GCNT2
GCNT3
GCNT4
GNPNAT1
GNPTAB
GNPTG
HEXA
HEXB
MAN1A1
MAN1A2
MAN1B1
MAN1C1
MAN2A1
MAN2A2
MAN2B1
MAN2B2
MAN2C1
MANBA
MANEA
MGAT1
MGAT2
MGAT3
MGAT4A
MGAT4B
MGAT4C
MGAT4D
MGAT5
MOGS
NAGA
NAGK
NAGPA
NEU1
NEU2
NEU3
NEU4
PRKCSH
RPN1
RPN2
ST3GAL1
ST3GAL2
ST3GAL3
ST3GAL4
ST3GAL5
ST3GAL6
ST6GAL1
ST6GAL2
ST6GALNAC1
ST6GALNAC2
ST6GALNAC3
ST6GALNAC4
ST6GALNAC5
ST6GALNAC6
ST8SIA1
ST8SIA2
ST8SIA3
ST8SIA4
ST8SIA5
ST8SIA6
STT3A
STT3B
UGCG
UGGT1
UGGT2
UGT8
Pathway Map
B4GALT5



General Information
Gene Symbol:   
B4GALT5
Protein name:   
Beta-1,4-galactosyltransferase 5
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT7
E.C. No:   
2.4.1.90
Organs:   
Reaction (SNFG):   
+
→
+ UDP
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• Weak enzyme activity. β4GalT5 mainly produces mono-galactosylated product. Prefers to transfer Gal to β1,4GlcNAc arm at α1,3-mannose.

Related Pathway
KEGG ORTHOLOGY:   
K09905
KEGG Pathway:   
ko00512 
Reactome:   
R-HSA-2022854
Related Database
Organism:   
Homo sapiens
Uniprot:   
O43286 
NCBI GeneID:   
9334
GlyMap GeneID:   
9334
DNA aRefSeq:   
NM_004776.3 
Protein RefSeq:   
NP_004767.1 
BRENDA:   
Kinetic info.
OMIM number:   
604016
GeneCards database:   
B4GALT5
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GlcNAc(b1-?) or Glc(b1-?)Cer
Product:  
     Gal(b1-4)GlcNAc(b1-?) or Gal(b1-4)Glc(b1-?)Cer
Constraints:  
     !@GlcNAc(b1-6)[GlcNAc(b1-3)]GalNAc(a1-r)&!Gal(b1-3)GlcNAc(b1-?)&!@GlcNAc(b1-4)...Man(b1-4)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    5.75e-04    (pmol/cell • hr)       KM:    1.50e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 50 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000371711 FOXP1 ZNF623 JUNB AR GATA1 CTCF KMT2A RUNX1 KLF5 ASCL1 CDX2 ZNF335 DLX1 ZNF816 USF1 MYCN ZNF24 FOSL1 FOSL1 MXI1 MXI1 NCOA2 NCOA2 NCOA2 ZBTB17 ZBTB17 FOS ESRRA ZBTB11 ZNF629 KLF17 L3MBTL4 ZNF580 BACH2 SSRP1 KLF16 SMAD4 E4F1 E4F1 TCF3 GATA6 GATA6 FLI1 NKX2-1 MAFK MAFK NFATC3 ZNF574 SMARCC1 POU2F2 ZEB1 HOXC9 ZKSCAN8 PRDM10 PRDM10 IRF4 MAX MAX ZSCAN22 BATF MXD4 HOMEZ HOMEZ HOMEZ ZBTB26 ARID3A ARID3A KLF7 SP4 ATF7 ATF7 ATF7 TEAD1 DMAP1 ZNF644 ZNF644 GLIS1 ARID4B ARID4B KLF10 KLF10 TFE3 SOX5 TEAD3 RCOR1 SOX13 SOX13 ZNF143 HSF1 GATAD1 IRF3 ZNF48 KLF16 KAT8 ZBTB33 ZBTB33 ZBTB33 SMAD4 SMAD4 ELF1 KLF12 FOSL1 FOSL1 EGR2 HBP1 CEBPG NFIL3 NR2F6 NR2F6 PAX5 CREM CREM SOX9 NFE2 KLF9 MAZ E2F6 E2F6 SKI EGR3 FOXP2 ZBTB14 SMAD1 USF2 EBF1 E2F4 SP2 BHLHE40 GATA1 GATA1 NFE2 CEBPD USF1 KLF9 EGR1 EGR1 RUNX1 TFAP4 VEZF1 CTCFL TAF1 TCF7L1 SP1 E2F6
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4479 917-924 -0.575 97.0 -0.57 97.0 noncon
hsa-miR-125b-1-3p 817-824 -0.547 99.0 -0.543 99.0 noncon
hsa-miR-210-3p 154-161 -0.511 97.0 -0.511 98.0 con
hsa-miR-659-3p 3360-3367 -0.492 99.0 -0.002 46.0 noncon
hsa-miR-4537 2182-2189 -0.486 97.0 -0.482 98.0 noncon
hsa-miR-6078 2556-2563 -0.467 99.0 -0.463 99.0 noncon
hsa-miR-6771-5p 755-762 -0.461 96.0 -0.458 97.0 noncon
hsa-miR-891a-5p 2030-2037 -0.456 96.0 -0.452 97.0 noncon
hsa-miR-6746-5p 754-761 -0.451 99.0 -0.448 99.0 noncon
hsa-miR-5188 47-53 -0.446 95.0 -0.446 97.0 noncon
hsa-miR-152-3p 3222-3229 -0.44 98.0 -0.437 99.0 con
hsa-miR-148b-3p 3222-3229 -0.44 98.0 -0.437 99.0 con
hsa-miR-148a-3p 3222-3229 -0.44 98.0 -0.437 99.0 con
hsa-miR-7846-3p 940-947 -0.438 96.0 -0.435 97.0 noncon
hsa-miR-3679-3p 134-141 -0.437 97.0 -0.437 98.0 noncon
hsa-miR-942-3p 624-631 -0.426 99.0 -0.423 99.0 noncon
hsa-miR-574-3p 3142-3149 -0.424 96.0 -0.421 97.0 noncon
hsa-miR-6803-3p 777-784 -0.411 95.0 -0.408 96.0 noncon
hsa-miR-501-3p 2331-2338 -0.408 98.0 -0.405 99.0 con
hsa-miR-502-3p 2331-2338 -0.408 98.0 -0.405 99.0 con
hsa-miR-6742-5p 282-289 -0.404 98.0 -0.404 98.0 noncon
hsa-miR-218-1-3p 2875-2882 -0.346 96.0 -0.343 97.0 noncon
hsa-miR-1288-3p 44-50 -0.343 96.0 -0.343 97.0 noncon
hsa-miR-6786-5p 26-32 -0.333 94.0 -0.333 95.0 noncon
hsa-miR-6796-5p 283-289 -0.333 95.0 -0.333 96.0 noncon
hsa-miR-4721 1794-1801 -0.329 95.0 -0.327 96.0 noncon
hsa-miR-3175 268-275 -0.326 96.0 -0.326 97.0 noncon
hsa-miR-3141 1857-1863 -0.324 93.0 -0.322 94.0 noncon
hsa-miR-605-3p 1947-1954 -0.322 96.0 -0.32 97.0 noncon
hsa-miR-4493 398-405 -0.317 96.0 -0.317 97.0 noncon
hsa-miR-6887-3p 545-552 -0.317 92.0 -0.315 94.0 noncon
hsa-miR-4751 3049-3055 -0.314 95.0 -0.312 96.0 noncon
hsa-miR-2117 91-98 -0.312 96.0 -0.312 97.0 noncon
hsa-miR-505-5p 662-669 -0.305 97.0 -0.303 98.0 noncon
hsa-miR-26b-3p 93-100 -0.302 96.0 -0.302 97.0 noncon
hsa-miR-4759 3267-3273 -0.3 97.0 -0.298 98.0 noncon
hsa-miR-7156-5p 2653-2660 -0.299 98.0 -0.297 98.0 noncon
hsa-miR-767-5p 2329-2336 -0.295 97.0 -0.293 98.0 noncon
hsa-miR-5189-3p 707-714 -0.294 94.0 -0.292 96.0 noncon
hsa-miR-4445-5p 588-595 -0.294 95.0 -0.292 96.0 noncon
hsa-miR-1199-3p 915-921 -0.288 90.0 -0.286 91.0 noncon
hsa-miR-6769a-5p 418-425 -0.282 96.0 -0.282 97.0 noncon
hsa-miR-8071 2769-2776 -0.277 95.0 -0.275 96.0 noncon
hsa-miR-6746-5p 1548-1554 -0.277 91.0 -0.275 93.0 noncon
hsa-miR-135a-5p 622-628 -0.274 94.0 -0.272 95.0 con
hsa-miR-135b-5p 622-628 -0.274 94.0 -0.272 95.0 con
hsa-miR-1226-5p 1795-1802 -0.274 96.0 -0.272 97.0 noncon
hsa-miR-6865-3p 539-545 -0.272 94.0 -0.27 96.0 noncon
hsa-miR-6769b-5p 25-31 -0.271 95.0 -0.271 96.0 noncon
hsa-miR-196a-3p 2581-2588 -0.271 93.0 -0.269 95.0 noncon
hsa-miR-483-3p.1 1897-1904 -0.268 96.0 -0.266 97.0 noncon
hsa-miR-19a-3p 3220-3226 -0.267 92.0 -0.265 94.0 con
hsa-miR-19b-3p 3220-3226 -0.267 92.0 -0.265 94.0 con
hsa-miR-6769b-5p 418-425 -0.264 95.0 -0.264 96.0 noncon
hsa-miR-6769a-5p 25-31 -0.261 95.0 -0.261 96.0 noncon
hsa-miR-503-5p 260-266 -0.257 97.0 -0.257 97.0 noncon
hsa-miR-3677-5p 695-701 -0.251 94.0 -0.249 96.0 noncon
hsa-miR-450a-2-3p 1782-1789 -0.251 90.0 -0.249 93.0 noncon
hsa-miR-6763-5p 269-275 -0.25 92.0 -0.25 94.0 noncon