GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 

Glycogene class
ABO
ALG1
ALG10
ALG10B
ALG11
ALG12
ALG13
ALG14
ALG1L
ALG1L2
ALG2
ALG3
ALG5
ALG6
ALG8
ALG9
B3GALNT2
B3GALT2
B3GAT1
B3GAT2
B3GNT2
B3GNT4
B3GNT7
B3GNT8
B4GALNT2
B4GALNT3
B4GALNT4
B4GALT1
B4GALT2
B4GALT3
B4GALT4
B4GALT5
CHST1
CHST10
CHST2
CHST3
CHST4
CHST5
CHST8
CHST9
CMAS
CTBS
DAD1
DDOST
DPAGT1
DPM1
DPM2
DPM3
EDEM1
EDEM2
EDEM3
ENGASE
FPGT
FUCA1
FUT1
FUT10
FUT11
FUT2
FUT3
FUT4
FUT5
FUT6
FUT7
FUT8
FUT9
GAL3ST2
GAL3ST3
GANAB
GCNT2
GNPNAT1
GNPTAB
GNPTG
MAN1A1
MAN1A2
MAN1B1
MAN1C1
MAN2A1
MAN2A2
MAN2B1
MAN2B2
MAN2C1
MANBA
MANEA
MGAT1
MGAT2
MGAT3
MGAT4A
MGAT4B
MGAT4C
MGAT4D
MGAT5
MOGS
NAGK
NAGPA
NEU1
NEU2
NEU3
NEU4
PRKCSH
RPN1
RPN2
ST3GAL3
ST3GAL4
ST3GAL6
ST6GAL1
ST6GAL2
ST8SIA2
ST8SIA3
ST8SIA4
STT3A
STT3B
UGGT1
UGGT2
Pathway Map

ALG9



General Information
Gene Symbol:   
ALG9
Protein name:   
Alpha-1,2-mannosyltransferase ALG9
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
E.C. No:   
Organs:   
Reaction (SNFG):   
+
+ P-Dol
+
+ P-Dol
Reaction (IUBMB):   
No specified reaction.

About

• Extension of Man5GlcNAc2-P-P-Dol in the ER lumen involves the addition of four Man (by ALG3 [α(1,3)ManT], ALG9 [α(1,2)ManT], ALG12 [α(1,6)ManT] followed by ALG9 [α(1,2)ManT] again) and three Glc residues (by ALG6 [α(1,3)GlcT], ALG8 [α(1,3)GlcT] and ALG10 [α(1,2)GlcT]).
• In all instances the transfer uses either Dol-P-Man or Dol-P-Glc as donor.
• Dol-P-Man is formed from GDP-Man using enzymes DPM1,2,3
• Dol-P-Glc is formed from UDP-Glc using ALG5


Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
Reactome:   

Related Database
Organism:   
Homo sapiens
Uniprot:   
NCBI GeneID:   
GlyMap GeneID:   
DNA aRefSeq:   
Protein RefSeq:   
BRENDA:   
OMIM number:   
GeneCards database:   

Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     ER
Substrate:  
     Man(a1-3)Man(a1-6) or Man(a1-6)...Man(a1-6)
Product:  
     Man(a1-2)Man(a1-3)Man(a1-6) or Man(a1-2)Man(a1-6)...Man(a1-6)
Constraints:  
     nMan>6&nMan(a1-6)=2

Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN











Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data

miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-494-5p 23-30 -0.565 99.0 -0.565 99.0 noncon
hsa-miR-410-5p 23-30 -0.549 99.0 -0.549 99.0 noncon
hsa-miR-323b-5p 23-30 -0.549 99.0 -0.549 99.0 noncon
hsa-miR-513a-5p 4164-4171 -0.521 99.0 -0.015 56.0 noncon
hsa-miR-1296-5p 234-241 -0.513 98.0 -0.037 44.0 noncon
hsa-miR-6866-5p 651-658 -0.494 98.0 -0.036 61.0 noncon
hsa-miR-4738-5p 2113-2120 -0.482 97.0 -0.017 32.0 noncon
hsa-miR-6829-5p 3476-3483 -0.46 99.0 -0.014 48.0 noncon
hsa-miR-4512 233-240 -0.447 99.0 -0.033 57.0 noncon
hsa-miR-5008-3p 242-249 -0.414 98.0 -0.031 63.0 noncon
hsa-miR-7151-3p 77-84 -0.411 99.0 -0.411 99.0 noncon
hsa-miR-370-5p 627-634 -0.397 99.0 -0.03 71.0 noncon
hsa-miR-6781-5p 3217-3224 -0.391 91.0 -0.012 19.0 noncon
hsa-miR-5095 77-84 -0.382 98.0 -0.382 99.0 noncon
hsa-miR-1193 627-634 -0.376 98.0 -0.028 68.0 noncon
hsa-miR-6737-3p 242-249 -0.353 97.0 -0.027 61.0 noncon
hsa-miR-6851-5p 532-539 -0.35 96.0 -0.026 48.0 noncon
hsa-miR-4317 1001-1008 -0.33 96.0 -0.018 55.0 noncon
hsa-miR-7157-3p 242-249 -0.329 97.0 -0.025 59.0 noncon
hsa-miR-3164 167-174 -0.329 97.0 -0.026 56.0 noncon
hsa-miR-6765-3p 4031-4038 -0.328 98.0 -0.01 60.0 noncon
hsa-miR-4802-5p 1292-1299 -0.323 97.0 -0.012 46.0 noncon
hsa-miR-6820-3p 167-174 -0.321 97.0 -0.026 55.0 noncon
hsa-miR-6757-3p 2238-2245 -0.321 98.0 -0.012 52.0 noncon
hsa-miR-765 745-752 -0.318 95.0 -0.024 48.0 noncon
hsa-miR-8082 2725-2732 -0.309 96.0 -0.01 38.0 noncon
hsa-miR-187-5p 4189-4196 -0.302 98.0 -0.009 36.0 noncon
hsa-miR-7-5p 4104-4110 -0.302 97.0 -0.009 55.0 con
hsa-miR-6880-5p 434-441 -0.295 93.0 -0.023 46.0 noncon
hsa-miR-7973 1570-1577 -0.292 93.0 -0.011 43.0 noncon
hsa-miR-601 299-305 -0.291 90.0 -0.022 39.0 noncon
hsa-miR-152-5p 315-322 -0.29 95.0 -0.022 47.0 noncon
hsa-miR-1278 1545-1552 -0.288 96.0 -0.011 43.0 noncon
hsa-miR-367-5p 3933-3940 -0.288 95.0 -0.009 49.0 noncon
hsa-miR-7847-3p 3822-3829 -0.28 93.0 -0.009 36.0 noncon
hsa-miR-4419a 2803-2810 -0.28 94.0 -0.009 33.0 noncon
hsa-miR-7162-5p 4059-4066 -0.279 95.0 -0.009 35.0 noncon
hsa-miR-4527 462-468 -0.279 94.0 -0.022 53.0 noncon
hsa-miR-6759-3p 95-101 -0.276 95.0 -0.276 96.0 noncon
hsa-miR-4755-3p 387-394 -0.276 94.0 -0.021 56.0 noncon
hsa-miR-3681-3p 3718-3725 -0.274 96.0 -0.009 46.0 con
hsa-miR-6828-3p 312-319 -0.272 96.0 -0.021 62.0 noncon
hsa-miR-4270 61-67 -0.271 96.0 -0.271 97.0 noncon
hsa-miR-4501 169-175 -0.269 96.0 -0.022 68.0 noncon
hsa-miR-107 4042-4049 -0.268 95.0 -0.009 36.0 con
hsa-miR-103a-3p 4042-4049 -0.268 95.0 -0.009 36.0 con
hsa-miR-4717-3p 750-757 -0.267 97.0 -0.021 64.0 noncon
hsa-miR-6871-3p 125-131 -0.266 95.0 -0.022 58.0 noncon
hsa-miR-6838-3p 569-576 -0.264 95.0 -0.021 52.0 noncon
hsa-miR-216a-3p 3718-3725 -0.264 96.0 -0.009 47.0 con
hsa-miR-516a-3p 4059-4066 -0.263 94.0 -0.009 36.0 noncon
hsa-miR-516b-3p 4059-4066 -0.263 94.0 -0.009 36.0 noncon
hsa-miR-676-3p 787-793 -0.262 96.0 -0.02 49.0 noncon
hsa-miR-1910-5p 1332-1339 -0.26 94.0 -0.01 42.0 noncon
hsa-miR-6857-5p 3327-3334 -0.258 96.0 -0.008 37.0 noncon
hsa-miR-3689d 532-539 -0.256 96.0 -0.02 55.0 noncon
hsa-miR-128-3p 3718-3725 -0.256 96.0 -0.008 47.0 con
hsa-miR-519d-5p 796-803 -0.254 96.0 -0.02 55.0 noncon
hsa-miR-370-5p 52-58 -0.252 95.0 -0.252 96.0 noncon
hsa-miR-1271-3p 3062-3069 -0.252 90.0 -0.008 32.0 noncon
hsa-miR-765 1290-1297 -0.251 91.0 -0.01 36.0 noncon