GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
A4GNT



General Information
Gene Symbol:   
A4GNT
Protein name:   
Alpha-1,4-N-acetylglucosaminyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT32
E.C. No:   
2.4.1.*
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• Transfers GlcNAc(α1-4) to βGal preferentially present in O-glycans forming GlcNAc(α1-4)Galβ-R or terminal αGlcNAc.
• The enzyme shows preference to act on core-2 glycan rather than core-1 glycans indicating a role for GlcNAc(β1-6) in promoting its activity. It extends the core-1 arm of O-glycans.
• It can act on both arms of core-2 glycan to corm structures like GlcNAc(α1-4)Gal(β1-4)GlcNAc(β1-6)[GlcNAc(α1-4)Gal(β1-3)]GalNAc.
• A4GNT is expressed in stomach, gall bladder, duodenum, and thus such structures are normally restricted to gland & gastric mucins, particulalry MUC6 with a small amount also being associated with MUC5AC.
• αGlcNAc is also expressed in pancreatic ducts showing gastric metaplasia or pancreatic intraepithelial neoplasia 1 (PanIN-1).
• A4gnt knockout mice revealed that αGlcNAc serves as a tumor suppressor of differentiated type of gastric adenocarcinoma. In addition, αGlcNAc protects gastric gland mucous cells from H. pylori infection. Thus, αGlcNAc plays a dual role to prevent the gastric mucosa from gastric tumorigenesis.

Related Pathway
KEGG ORTHOLOGY:   
K08251
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-913709
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9UNA3 
NCBI GeneID:   
51146
GlyMap GeneID:   
51146
DNA aRefSeq:   
NM_016161.2 
Protein RefSeq:   
NP_057245.1 
BRENDA:   
Kinetic info.
OMIM number:   
-
GeneCards database:   
A4GNT
Enzyme Specificity
Glycan Type:  
     O-linked (mucin type)
Compartment:  
     
Substrate:  
     Gal(b1-?)
Product:  
     GlcNAc(a1-4)Gal(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 12 10 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 4 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000236709 ZFX PPARG NFIC CTCF MYC ZNF143 DNMT3B
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-let-7e-3p 302-309 -0.723 99.0 -0.723 99.0 noncon
hsa-miR-3136-5p 524-531 -0.607 99.0 -0.607 99.0 noncon
hsa-miR-22-3p 397-404 -0.601 99.0 -0.601 99.0 con
hsa-miR-6772-5p 182-189 -0.569 99.0 -0.569 99.0 noncon
hsa-miR-6769a-5p 216-223 -0.56 99.0 -0.56 99.0 noncon
hsa-miR-4777-3p 470-477 -0.549 99.0 -0.549 99.0 noncon
hsa-miR-575 167-174 -0.54 99.0 -0.54 99.0 noncon
hsa-miR-891a-3p 52-59 -0.534 99.0 -0.534 99.0 noncon
hsa-miR-6769b-5p 216-223 -0.534 99.0 -0.534 99.0 noncon
hsa-miR-660-5p 381-388 -0.53 99.0 -0.53 99.0 noncon
hsa-miR-4439 524-531 -0.526 99.0 -0.526 99.0 noncon
hsa-miR-4676-5p 167-174 -0.516 99.0 -0.516 99.0 noncon
hsa-miR-148b-3p 325-332 -0.498 99.0 -0.498 99.0 noncon
hsa-miR-152-3p 325-332 -0.498 99.0 -0.498 99.0 noncon
hsa-miR-148a-3p 325-332 -0.498 99.0 -0.498 99.0 noncon
hsa-miR-4683 69-76 -0.464 99.0 -0.464 99.0 noncon
hsa-miR-6513-5p 155-162 -0.461 99.0 -0.461 99.0 noncon
hsa-miR-3619-3p 189-196 -0.451 99.0 -0.451 99.0 noncon
hsa-miR-6881-3p 254-261 -0.439 98.0 -0.439 99.0 noncon
hsa-miR-1301-3p 342-349 -0.415 98.0 -0.415 99.0 noncon
hsa-miR-5047 342-349 -0.415 98.0 -0.415 98.0 noncon
hsa-miR-567 178-185 -0.412 99.0 -0.412 99.0 noncon
hsa-miR-4264 331-338 -0.412 99.0 -0.412 99.0 noncon
hsa-miR-5089-5p 154-161 -0.401 99.0 -0.401 99.0 noncon
hsa-miR-873-5p.2 290-297 -0.381 99.0 -0.381 99.0 noncon
hsa-miR-7977 355-362 -0.361 99.0 -0.361 99.0 noncon
hsa-miR-6875-3p 372-379 -0.358 98.0 -0.358 99.0 noncon
hsa-miR-4455 183-189 -0.332 91.0 -0.332 94.0 noncon
hsa-miR-6882-3p 396-402 -0.322 96.0 -0.322 97.0 noncon
hsa-miR-4274 200-206 -0.318 97.0 -0.318 98.0 noncon
hsa-miR-4712-5p 386-392 -0.306 96.0 -0.306 97.0 noncon
hsa-miR-5591-3p 382-388 -0.302 96.0 -0.302 97.0 noncon
hsa-miR-500a-3p 262-268 -0.3 96.0 -0.3 97.0 noncon
hsa-miR-6503-3p 493-499 -0.296 96.0 -0.296 97.0 noncon
hsa-miR-770-5p 386-392 -0.295 96.0 -0.295 97.0 noncon
hsa-miR-4264 467-473 -0.292 97.0 -0.292 97.0 noncon
hsa-miR-6855-3p 524-530 -0.29 96.0 -0.29 97.0 noncon
hsa-miR-1324 138-144 -0.29 97.0 -0.29 98.0 noncon
hsa-miR-4776-5p 190-196 -0.289 95.0 -0.289 96.0 noncon
hsa-miR-3184-3p 481-487 -0.282 97.0 -0.282 98.0 noncon
hsa-miR-6792-3p 369-375 -0.279 95.0 -0.279 96.0 noncon
hsa-miR-4258 85-91 -0.275 91.0 -0.275 93.0 noncon
hsa-miR-6780a-3p 255-261 -0.273 96.0 -0.273 97.0 noncon
hsa-miR-200b-5p 435-441 -0.269 97.0 -0.269 98.0 noncon
hsa-miR-200a-5p 435-441 -0.269 97.0 -0.269 98.0 noncon
hsa-miR-4697-3p 327-333 -0.267 92.0 -0.267 94.0 noncon
hsa-miR-4513 524-530 -0.266 95.0 -0.266 96.0 noncon
hsa-miR-3121-3p 422-429 -0.265 99.0 -0.265 99.0 noncon
hsa-miR-6804-3p 262-268 -0.263 92.0 -0.263 95.0 noncon
hsa-miR-609 183-189 -0.261 92.0 -0.261 94.0 noncon
hsa-miR-627-3p 250-257 -0.26 97.0 -0.26 98.0 noncon
hsa-miR-7153-3p 187-193 -0.258 96.0 -0.258 97.0 noncon
hsa-miR-450a-1-3p 156-162 -0.257 96.0 -0.257 97.0 noncon