GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
GALNT12



General Information
Gene Symbol:   
GALNT12
Protein name:   
Polypeptide N-acetylgalactosaminyltransferase 12
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
CBM13
E.C. No:   
2.4.1.41
Organs:   
Reaction (SNFG):   
+ Ser →
+ UDP
+ Thr →
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• ppGalNAc-T12 is reported to be a follow-up enzyme that requires prior GalNAcylation of the peptide. Other follow-up enzymes include GALNT4, 7, 10, 17.

Related Pathway
KEGG ORTHOLOGY:   
K00710
KEGG Pathway:   
ko00512  ko01100 
Reactome:   
R-HSA-5083636
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q8IXK2 
NCBI GeneID:   
79695
GlyMap GeneID:   
79695
DNA aRefSeq:   
NM_024642.4 
Protein RefSeq:   
NP_078918.3 
BRENDA:   
Kinetic info.
OMIM number:   
610290
GeneCards database:   
GALNT12
Enzyme Specificity
Glycan Type:  
     O-linked (mucin type)
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Ser or Thr
Product:  
     GalNAc(a1-?)Ser or GalNAc(a1-?)Thr
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 60 50 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6769a-5p 85-92 -0.639 99.0 -0.639 99.0 noncon
hsa-miR-6769b-5p 85-92 -0.621 99.0 -0.621 99.0 noncon
hsa-miR-4540 612-619 -0.477 98.0 -0.365 97.0 noncon
hsa-miR-3147 52-59 -0.455 99.0 -0.455 99.0 noncon
hsa-miR-7851-3p 374-381 -0.425 98.0 -0.326 97.0 noncon
hsa-miR-122-5p 583-590 -0.413 99.0 -0.317 98.0 noncon
hsa-miR-1266-5p 927-934 -0.401 98.0 -0.309 98.0 noncon
hsa-miR-130a-5p 164-171 -0.384 97.0 -0.384 97.0 con
hsa-miR-7113-3p 774-781 -0.373 98.0 -0.288 98.0 noncon
hsa-miR-126-3p.1 378-384 -0.363 93.0 -0.28 88.0 noncon
hsa-miR-23a-3p 164-171 -0.362 96.0 -0.362 97.0 con
hsa-miR-124-3p.1 314-320 -0.362 97.0 -0.279 96.0 con
hsa-miR-23c 164-171 -0.362 96.0 -0.362 97.0 con
hsa-miR-23b-3p 164-171 -0.362 96.0 -0.362 97.0 con
hsa-miR-4653-5p 633-640 -0.348 98.0 -0.269 98.0 noncon
hsa-miR-380-5p 939-945 -0.346 96.0 -0.267 95.0 noncon
hsa-miR-6823-5p 925-932 -0.345 98.0 -0.267 97.0 noncon
hsa-miR-6867-3p 775-782 -0.344 97.0 -0.266 96.0 noncon
hsa-miR-563 939-945 -0.336 95.0 -0.26 94.0 noncon
hsa-miR-4518 927-934 -0.336 99.0 -0.26 98.0 noncon
hsa-miR-4536-5p 571-578 -0.334 94.0 -0.258 93.0 noncon
hsa-miR-2682-3p 115-122 -0.334 97.0 -0.334 98.0 noncon
hsa-miR-4732-3p 772-779 -0.334 98.0 -0.258 98.0 noncon
hsa-miR-3622a-5p 313-319 -0.329 98.0 -0.255 97.0 noncon
hsa-miR-7156-3p 100-106 -0.329 97.0 -0.329 97.0 noncon
hsa-miR-7975 488-495 -0.327 97.0 -0.253 97.0 noncon
hsa-miR-330-3p 108-115 -0.327 99.0 -0.327 99.0 noncon
hsa-miR-202-3p 441-448 -0.31 95.0 -0.24 94.0 noncon
hsa-miR-3921 633-640 -0.308 98.0 -0.239 97.0 noncon
hsa-miR-6514-5p 585-592 -0.308 98.0 -0.239 97.0 noncon
hsa-miR-6781-3p 115-122 -0.305 97.0 -0.305 98.0 noncon
hsa-miR-7156-5p 637-644 -0.3 98.0 -0.233 97.0 noncon
hsa-miR-2117 636-643 -0.284 95.0 -0.221 93.0 noncon
hsa-miR-4255 981-987 -0.284 97.0 -0.117 90.0 noncon
hsa-miR-1264 60-67 -0.281 98.0 -0.281 98.0 noncon
hsa-miR-1279 955-962 -0.279 99.0 -0.217 98.0 noncon
hsa-miR-3117-5p 768-775 -0.266 95.0 -0.207 93.0 noncon
hsa-miR-7156-5p 979-985 -0.261 97.0 -0.125 91.0 noncon
hsa-miR-4674 17-23 -0.26 95.0 -0.26 96.0 noncon
hsa-miR-4437 17-23 -0.253 90.0 -0.253 93.0 noncon
hsa-miR-3909 610-616 -0.252 91.0 -0.196 89.0 noncon
hsa-miR-4712-3p 324-330 -0.251 96.0 -0.196 95.0 noncon