GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Glycogene class
ABO
ALG1
ALG10
ALG10B
ALG11
ALG12
ALG13
ALG14
ALG1L
ALG1L2
ALG2
ALG3
ALG5
ALG6
ALG8
ALG9
B3GALNT2
B3GALT2
B3GAT1
B3GAT2
B3GNT2
B3GNT4
B3GNT7
B3GNT8
B4GALNT2
B4GALNT3
B4GALNT4
B4GALT1
B4GALT2
B4GALT3
B4GALT4
B4GALT5
CHST1
CHST10
CHST2
CHST3
CHST4
CHST5
CHST8
CHST9
CMAS
CTBS
DAD1
DDOST
DPAGT1
DPM1
DPM2
DPM3
EDEM1
EDEM2
EDEM3
ENGASE
FPGT
FUCA1
FUT1
FUT10
FUT11
FUT2
FUT3
FUT4
FUT5
FUT6
FUT7
FUT8
FUT9
GAL3ST2
GAL3ST3
GANAB
GCNT2
GNPNAT1
GNPTAB
GNPTG
MAN1A1
MAN1A2
MAN1B1
MAN1C1
MAN2A1
MAN2A2
MAN2B1
MAN2B2
MAN2C1
MANBA
MANEA
MGAT1
MGAT2
MGAT3
MGAT4A
MGAT4B
MGAT4C
MGAT4D
MGAT5
MOGS
NAGK
NAGPA
NEU1
NEU2
NEU3
NEU4
PRKCSH
RPN1
RPN2
ST3GAL3
ST3GAL4
ST3GAL6
ST6GAL1
ST6GAL2
ST8SIA2
ST8SIA3
ST8SIA4
STT3A
STT3B
UGGT1
UGGT2
Pathway Map
STT3A



General Information
Gene Symbol:   
STT3A
Protein name:   
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT66
E.C. No:   
2.4.99.18
Organs:   
Reaction (SNFG):   
→
Reaction (IUBMB):   
No specified reaction.
About

• A multi-membrane protein ER membrane associated protein complex that mediates the en-bloc transfer of 14 sugar LLO to N-X-S/T motif in nascent polypeptide
• STT3 is the catalytic core of this complex
• Stt3 forms a complex with the accessory membrane proteins DDOST/OST48, RPN1/ribophorin I, RPN2/ribophorin II, DAD1, Ost4, TUSC3/N33 and MGAT1/IAP in mammals for maximal activity.
• There are two STT3 genes: STT3A and STT3B. Human Stt3A and Stt3B have redundant functions in the co-translational N-glycosylation. Stt3B also mediates the post-translational N-glycosylation after some time, rather than just at the time when the protein enters the ER from the ribosome.
• Glc[0-3]Man[0-9]GlcNAc2β1-PP-Dol + Asn-Xaa-Ser/Thr -> Dol-PP + Glc[0-3]Man[0-9]GlcNAc2β1-Asn-Xaa-Ser/Thr.

Related Pathway
KEGG ORTHOLOGY:   
K07151
KEGG Pathway:   
ko00510  ko00513  ko01100  ko04141 
Reactome:   
R-HSA-446203
Related Database
Organism:   
Homo sapiens
Uniprot:   
P46977 
NCBI GeneID:   
3703
GlyMap GeneID:   
3703
DNA aRefSeq:   
NM_001278503.1 
Protein RefSeq:   
NP_001265432.1 
BRENDA:   
Kinetic info.
OMIM number:   
601134
GeneCards database:   
STT3A
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     ER
Substrate:  
     Glc(a1-2)Glc(a1-3)Man(a1-2)Man(a1-2)Man(a1-3)[Man(a1-2)Man(a1-6)[Man(a1-2)Man(a1-3)]Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc(a1-?)Dol-PP
Product:  
     Glc(a1-2)Glc(a1-3)Man(a1-2)Man(a1-2)Man(a1-3)[Man(a1-2)Man(a1-6)[Man(a1-2)Man(a1-3)]Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc(b1-?)Asn
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 50 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 600 500 400 300 200 100 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-124-3p.1 255-262 -0.576 99.0 -0.494 99.0 con
hsa-miR-649 187-194 -0.557 99.0 -0.52 99.0 noncon
hsa-miR-614 1321-1328 -0.537 99.0 -0.053 48.0 noncon
hsa-miR-190a-5p 748-755 -0.481 98.0 -0.048 65.0 noncon
hsa-miR-7152-3p 524-531 -0.466 99.0 -0.276 95.0 noncon
hsa-miR-190b 748-755 -0.465 98.0 -0.047 64.0 noncon
hsa-miR-133a-3p.1 523-529 -0.451 97.0 -0.371 96.0 con
hsa-miR-4677-5p 96-103 -0.451 99.0 -0.449 99.0 noncon
hsa-miR-3162-5p 351-358 -0.444 98.0 -0.383 97.0 noncon
hsa-miR-6778-3p 289-296 -0.411 98.0 -0.355 98.0 noncon
hsa-miR-6868-5p 311-318 -0.41 98.0 -0.354 98.0 noncon
hsa-miR-662 2081-2088 -0.404 96.0 -0.001 10.0 noncon
hsa-miR-4763-5p 2036-2043 -0.398 94.0 -0.001 21.0 noncon
hsa-miR-676-5p 53-60 -0.371 99.0 -0.371 99.0 noncon
hsa-miR-6836-3p 290-297 -0.366 98.0 -0.317 98.0 noncon
hsa-miR-217 85-91 -0.363 99.0 -0.363 99.0 con
hsa-miR-6739-3p 97-104 -0.361 98.0 -0.36 98.0 noncon
hsa-miR-3679-3p 234-240 -0.361 96.0 -0.334 96.0 noncon
hsa-miR-3975 1502-1509 -0.36 99.0 -0.037 67.0 noncon
hsa-miR-196b-5p 903-910 -0.358 97.0 -0.037 59.0 noncon
hsa-miR-196a-5p 903-910 -0.358 97.0 -0.037 59.0 noncon
hsa-miR-5591-5p 349-355 -0.357 96.0 -0.31 95.0 noncon
hsa-miR-6862-3p 1492-1499 -0.352 97.0 -0.037 68.0 noncon
hsa-miR-2116-5p 95-102 -0.34 99.0 -0.339 99.0 noncon
hsa-miR-6784-3p 1492-1499 -0.339 97.0 -0.035 67.0 noncon
hsa-miR-4433a-5p 521-527 -0.325 96.0 -0.269 96.0 noncon
hsa-miR-582-3p 1605-1612 -0.325 98.0 -0.029 63.0 noncon
hsa-miR-5582-5p 254-260 -0.319 97.0 -0.277 97.0 noncon
hsa-miR-6503-5p 328-334 -0.317 95.0 -0.275 95.0 noncon
hsa-miR-4527 328-334 -0.316 95.0 -0.275 95.0 noncon
hsa-miR-6807-3p 85-91 -0.316 98.0 -0.316 98.0 con
hsa-miR-124-3p.2 256-262 -0.315 91.0 -0.274 91.0 con
hsa-miR-5009-3p 44-51 -0.315 98.0 -0.315 99.0 noncon
hsa-miR-6511b-5p 994-1001 -0.315 96.0 -0.033 67.0 noncon
hsa-miR-8065 417-423 -0.313 93.0 -0.272 93.0 noncon
hsa-miR-6792-3p 1369-1375 -0.31 96.0 -0.033 54.0 noncon
hsa-miR-4255 1899-1906 -0.31 98.0 -0.028 72.0 noncon
hsa-miR-6726-5p 349-355 -0.31 94.0 -0.269 93.0 noncon
hsa-miR-4300 349-355 -0.302 94.0 -0.262 94.0 noncon
hsa-miR-6811-5p 994-1001 -0.296 96.0 -0.031 65.0 noncon
hsa-miR-642b-5p 1361-1368 -0.292 95.0 -0.031 62.0 noncon
hsa-miR-6807-5p 489-496 -0.292 99.0 -0.254 99.0 noncon
hsa-miR-4762-5p 1510-1517 -0.286 98.0 -0.03 74.0 noncon
hsa-miR-660-3p 1367-1374 -0.285 97.0 -0.03 61.0 noncon
hsa-miR-548av-3p 87-93 -0.284 98.0 -0.284 99.0 noncon
hsa-miR-939-3p 2182-2189 -0.283 96.0 0.0 40.0 noncon
hsa-miR-3152-5p 291-298 -0.28 94.0 -0.244 95.0 noncon
hsa-miR-376b-3p 1059-1066 -0.279 94.0 -0.03 50.0 noncon
hsa-miR-376a-3p 1059-1066 -0.279 94.0 -0.03 50.0 noncon
hsa-miR-328-3p 156-162 -0.277 94.0 -0.264 95.0 con
hsa-miR-1273c 1662-1668 -0.276 92.0 -0.025 37.0 noncon
hsa-miR-920 349-355 -0.273 95.0 -0.238 95.0 noncon
hsa-miR-3120-5p 428-434 -0.27 96.0 -0.235 96.0 noncon
hsa-miR-4279 1007-1014 -0.262 96.0 -0.028 74.0 noncon
hsa-miR-371b-3p 1444-1450 -0.262 92.0 -0.028 54.0 noncon
hsa-miR-2355-3p 1276-1283 -0.257 91.0 -0.027 51.0 noncon
hsa-miR-506-3p 256-262 -0.253 90.0 -0.22 91.0 con
hsa-miR-3607-3p 424-431 -0.25 97.0 -0.218 97.0 noncon
hsa-miR-448 83-89 -0.25 95.0 -0.25 97.0 con