GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 

Glycogene class
A4GNT
ABO
B3GALNT2
B3GALT1
B3GALT2
B3GALT5
B3GAT1
B3GAT2
B3GLCT
B3GNT2
B3GNT3
B3GNT4
B3GNT6
B3GNT7
B4GALNT2
B4GALNT3
B4GALNT4
B4GALT1
B4GALT2
B4GALT3
B4GALT4
B4GALT5
C1GALT1
C1GALT1C1
CHST1
CHST10
CHST2
CHST3
CHST4
CHST5
CHST8
CHST9
CMAS
EOGT
FKRP
FKTN
FPGT
FUCA1
FUT1
FUT2
FUT3
FUT4
FUT5
FUT6
FUT7
FUT9
GAL3ST2
GAL3ST3
GAL3ST4
GALNT1
GALNT10
GALNT11
GALNT12
GALNT13
GALNT14
GALNT15
GALNT16
GALNT17
GALNT18
GALNT2
GALNT3
GALNT4
GALNT5
GALNT6
GALNT7
GALNT8
GALNT9
GALNTL6
GCNT1
GCNT2
GCNT3
GCNT4
GXYLT1
GXYLT2
LFNG
MFNG
MGAT5B
NEU1
NEU2
NEU3
NEU4
OGT
POFUT1
POFUT2
POGLUT1
POGLUT2
POGLUT3
POMGNT1
POMGNT2
POMT1
POMT2
RFNG
RXYLT1
ST3GAL1
ST3GAL2
ST3GAL3
ST3GAL4
ST3GAL6
ST6GAL1
ST6GAL2
ST6GALNAC1
ST6GALNAC2
ST6GALNAC3
ST6GALNAC4
ST8SIA2
ST8SIA3
ST8SIA4
ST8SIA6
TMTC1
TMTC2
TMTC3
TMTC4
XXYLT1
Pathway Map

GAL3ST2



General Information
Gene Symbol:   
GAL3ST2
Protein name:   
Galactose-3-O-sulfotransferase 2
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
Organs:   
Reaction (SNFG):   
PAPS +
+ PAP
PAPS +
+ PAP
Reaction (IUBMB):   
No specified reaction.

About

• There are 4 enzymes that perform 3-O-sulfation of Gal.
• Gal3ST1: Acts on GalCer and LacCer to create sulfated glycolipids. Catalyzes the synthesis of sulfatides and seminolpids.
• Gal3ST2: Galβ1,4GlcNAc of O- and N-glycan, can act on sulfated substrates to form bisulfated carbohydrates like 3′,6-disulfo LewisX and 3′,6'-disulfo LewisX. This can also form 3'-sulfo-Le a and 3-O-sulfo-LeX determinants and it can act on lipid substrates
• Gal3ST3 Galβ1,4GlcNAc of O- and N-glycan and Galβ1,4GalNAc of GloboH structure. Can act on sulfated substrates to form bisulfated carbohydrates
• Gal3ST4 Galβ1,4GalNAc of Globo glycolipids and glycopeptides


Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
Reactome:   

Related Database
Organism:   
Homo sapiens
Uniprot:   
NCBI GeneID:   
GlyMap GeneID:   
DNA aRefSeq:   
Protein RefSeq:   
BRENDA:   
OMIM number:   
GeneCards database:   

Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-4)GlcNAc(b1-?) or Gal(b1-4)GlcNAc(b1-? [6S])
Product:  
     Gal(b1-4 [3S])GlcNAc(b1-?) or Gal(b1-4 [3S])GlcNAc(b1-? [6S])

Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN











Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 10 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 8 6 4 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000192314 ENST00000610560 ENST00000632847 TFAP4 GRHL2 MECOM RUNX1


miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6886-5p 26-33 -0.866 99.0 -0.866 99.0 noncon
hsa-miR-4707-5p 66-73 -0.576 99.0 -0.001 20.0 noncon
hsa-miR-6799-3p 79-85 -0.436 98.0 -0.001 31.0 noncon
hsa-miR-138-5p 34-40 -0.405 96.0 -0.405 97.0 noncon
hsa-miR-3173-5p 79-85 -0.403 98.0 -0.001 33.0 noncon
hsa-miR-4669 30-36 -0.385 99.0 -0.385 99.0 noncon
hsa-miR-4487 36-42 -0.381 98.0 -0.381 98.0 noncon
hsa-miR-6779-3p 80-86 -0.333 98.0 -0.001 25.0 noncon
hsa-miR-6763-3p 58-64 -0.31 90.0 -0.001 10.0 noncon
hsa-miR-7978 33-39 -0.292 96.0 -0.292 97.0 noncon
hsa-miR-4743-5p 114-120 -0.29 91.0 -0.001 6.0 noncon
hsa-miR-5739 45-51 -0.288 90.0 -0.001 9.0 noncon
hsa-miR-4695-5p 22-28 -0.267 95.0 -0.267 97.0 noncon
hsa-miR-4707-5p 58-64 -0.264 90.0 -0.001 18.0 noncon
hsa-miR-28-5p 38-44 -0.261 95.0 -0.001 21.0 noncon