GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
B3GLCT



General Information
Gene Symbol:   
B3GLCT
Protein name:   
Beta-1,3-glucosyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT31
E.C. No:   
2.4.1.*
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• During O-Fuc modification of TSR1 (thrombospondin type 1) repeat, the first step involves the transfer of fuc by Pofut2 to Ser/Thr in this domain. The absence of Pofut2 in mice results in early embryonic lethality with defects in gastrulation. 
• The next step involves the transfers glucose to O-Fuc by a gene encoding the β3-glucosyltransferase, B3GLCT or B3GALTL. Mutations in B3GLCT in humans results in a multifaceted severe developmental disorder known as Peters-plus syndrome.
• Besides O-fuc, and C-mannosylation also occur on TSR1/thrombospondin-1 domains.

Related Pathway
KEGG ORTHOLOGY:   
K13675
KEGG Pathway:   
ko00514 
Reactome:   
R-HSA-5083635
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q6Y288 
NCBI GeneID:   
145173
GlyMap GeneID:   
145173
DNA aRefSeq:   
NM_194318.3 
Protein RefSeq:   
NP_919299.3 
BRENDA:   
Kinetic info.
OMIM number:   
610308
GeneCards database:   
B3GLCT
Enzyme Specificity
Glycan Type:  
     O-linked (other)
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Fuc(a1-?)Ser|Thr
Product:  
     Glc(b1-3)Fuc(a1-?)Ser|Thr
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 8 6 4 2 0 RPKM
Expression Data in Cells
No expression data available.
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6846-3p 165-172 -0.581 99.0 -0.581 99.0 noncon
hsa-miR-7973 167-174 -0.443 98.0 -0.443 98.0 noncon
hsa-miR-6840-3p 495-502 -0.396 99.0 -0.247 99.0 noncon
hsa-miR-1185-5p 2495-2502 -0.392 98.0 -0.172 88.0 noncon
hsa-miR-3679-5p 2495-2502 -0.39 98.0 -0.172 90.0 noncon
hsa-miR-7152-3p 2141-2148 -0.381 97.0 -0.168 89.0 noncon
hsa-miR-7-5p 2542-2549 -0.372 98.0 -0.164 94.0 noncon
hsa-miR-4504 288-295 -0.361 98.0 -0.361 99.0 noncon
hsa-miR-135b-5p 1239-1246 -0.359 97.0 -0.185 91.0 con
hsa-miR-135a-5p 1239-1246 -0.359 97.0 -0.185 91.0 con
hsa-miR-6825-3p 1945-1951 -0.356 96.0 -0.158 84.0 noncon
hsa-miR-6776-3p 595-602 -0.349 98.0 -0.219 95.0 noncon
hsa-miR-3925-3p 40-47 -0.349 96.0 -0.349 97.0 noncon
hsa-miR-6780a-3p 153-160 -0.348 98.0 -0.348 98.0 noncon
hsa-miR-1263 307-313 -0.348 95.0 -0.348 97.0 noncon
hsa-miR-6868-5p 754-761 -0.334 97.0 -0.21 93.0 noncon
hsa-miR-4441 702-709 -0.33 98.0 -0.208 94.0 noncon
hsa-let-7e-3p 1697-1703 -0.328 89.0 -0.17 78.0 noncon
hsa-miR-6754-5p 702-709 -0.325 98.0 -0.205 94.0 noncon
hsa-miR-628-5p 810-817 -0.318 97.0 -0.201 95.0 noncon
hsa-miR-1539 27-33 -0.312 92.0 -0.312 93.0 noncon
hsa-miR-5004-5p 768-775 -0.308 95.0 -0.195 88.0 noncon
hsa-miR-6887-3p 1903-1910 -0.302 91.0 -0.135 77.0 noncon
hsa-miR-1224-5p 769-776 -0.297 98.0 -0.188 96.0 noncon
hsa-miR-4270 702-709 -0.296 97.0 -0.187 92.0 noncon
hsa-miR-4753-5p 1495-1502 -0.294 96.0 -0.153 88.0 noncon
hsa-miR-1912 2464-2470 -0.293 98.0 -0.131 91.0 noncon
hsa-miR-892a 994-1001 -0.291 97.0 -0.152 92.0 noncon
hsa-miR-4324 830-837 -0.286 97.0 -0.181 94.0 noncon
hsa-miR-505-3p.1 790-797 -0.283 91.0 -0.179 83.0 noncon
hsa-miR-6755-5p 1994-2000 -0.277 91.0 -0.125 79.0 noncon
hsa-miR-3653-5p 173-179 -0.274 98.0 -0.274 99.0 noncon
hsa-miR-124-3p.1 1742-1748 -0.274 94.0 -0.144 89.0 con
hsa-miR-6723-5p 2576-2582 -0.271 96.0 -0.122 87.0 noncon
hsa-miR-4531 255-262 -0.269 96.0 -0.269 97.0 noncon
hsa-miR-6826-3p 1904-1911 -0.268 92.0 -0.121 82.0 noncon
hsa-miR-124-5p 991-998 -0.263 92.0 -0.138 80.0 noncon
hsa-miR-4999-5p 269-276 -0.262 93.0 -0.262 95.0 noncon
hsa-miR-3972 1689-1695 -0.261 94.0 -0.137 88.0 noncon
hsa-miR-491-3p 1117-1124 -0.26 92.0 -0.136 84.0 noncon
hsa-miR-4275 2512-2519 -0.26 98.0 -0.117 94.0 noncon
hsa-miR-1197 767-773 -0.259 96.0 -0.165 93.0 noncon
hsa-miR-4529-5p 1493-1499 -0.257 90.0 -0.135 83.0 noncon
hsa-miR-6859-3p 166-172 -0.257 89.0 -0.257 92.0 noncon
hsa-miR-3158-3p 709-716 -0.256 95.0 -0.163 91.0 noncon
hsa-miR-6828-3p 2386-2393 -0.256 95.0 -0.116 87.0 noncon
hsa-miR-378j 2349-2356 -0.252 95.0 -0.114 86.0 noncon
hsa-miR-4717-5p 50-56 -0.251 95.0 -0.251 96.0 noncon