Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (, Yusen Zhou ( or Ted Groth (

Create glycosylation pathway
Custom database generation

Pathway Map


General Information
Gene Symbol:   
Protein name:   
Protein O-glucosyltransferase 1
Enzyme Class:   
CAZy Family:   
E.C. No:   
Reaction (SNFG):   
+ Ser
+ Ser
Reaction (IUBMB):   
No specified reaction.


• POGLUT1 is an ER resident, soluble, protein with O-Glucosyltransferase activity. It adds β-linked Glc to hydroxyl gp. of Ser specifically in EGF repeats.
• Rumi is the gene encoding for POGLUT1 activity. Rumi is essential for Notch signaling, and Rumi dysregulation is linked to several human diseases.
• The consensus sequence for this enzyme activity in EGF is C1-X-S-X-P/A-C2, where C1 and C2 are the first and second conserved Cys of the EGF repeat. Such a motif is predicted to occur in 40 proteins in mammals are 40 mammalian proteins.
• While initially identified in Factor VII and IX, it is heavily glycosylated on Notch. This is thus a penetrating gene that is E9.5 lethal in mice.
• O-glucose is typically extended by two α3-linked xyloses to the trisaccharide, Xyl(α1-3)Xyl(α1-3)Glcβ1-O-Ser in mammals, although mono- and di-saccharide forms have also been seen. Here, glucoside xylosyltransferases 1 and 2 (GXYLT1 and GXYLT2) adds the first α3-linked xylose to O-glucose on EGF repeats, and xyloside xylosytransferase 1 (XXTLT1) adds the second xylose to Xyl-Glc disaccharides on EGF repeats.
• POGLUT1 is overexpressed in some human myelodysplastic syndrome patients.
• POGLUT1 may also transfer Xyl directly to the EGF domain if the consensus sequence is C1-X-S-S-P/A-C2. Thus, it also exhibits POXYLT activity.

Related Pathway
KEGG Pathway:   

Related Database
Homo sapiens
NCBI GeneID:   
GlyMap GeneID:   
DNA aRefSeq:   
Protein RefSeq:   
OMIM number:   
GeneCards database:   

Enzyme Specificity
Glycan Type:  
     O-linked (other)
     Glc(b1-?)Ser or Xyl(a1-?)Ser

Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN

Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data