GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ST6GALNAC1



General Information
Gene Symbol:   
ST6GALNAC1
Protein name:   
Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 1
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT29
E.C. No:   
2.4.99.3
Organs:   
Reaction (SNFG):   
+
→
+ CMP
Reaction (IUBMB):   
No specified reaction.
About

• Important enzyme forming the sialyl-Tn antigen (NeuAc(α2-6)GalNAc-O-Ser/Thr) on cancer glycoproteins.
• Both ST6GalNAc-I and -II use GalNAc-O-Ser/Thr, Galβl-3GalNAc-O-Ser/Thr, and NeuAcα2-3Galβl-3GalNAc-O-Ser/Thr. However, ST6GalNac-I shows preference to make sialyl-Tn antigen, while ST6GalNAc-II prefers sialyl T-antigen.
• Only ST6GalNAc-I and -II may form sialyl-Tn antigen since ST6GalNAc-III-V only utilize Neu5Acα2-3Galβl-3GalNAc substrates.
• ST6GalNAc-I gene was limited in the submaxillary gland, mammary gland, colon, and spleen.
• ST6GalNAc-I and ST6GalNAc-II and ST3Gal-I and ST3Gal-II contain conserved Kurosawa motif (Cys-Xaa70-85-Cys-Xaα2-Cys-Ala-Val-Val-Gly-Asn-Xaa120-170-Cys; Xaa denotes any amino acid residue) and thus act on Galβ1-3GalNAc acceptor substrates.

Related Pathway
KEGG ORTHOLOGY:   
K03479
KEGG Pathway:   
ko00512  ko01100 
Reactome:   
R-HSA-4085001
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9NSC7 
NCBI GeneID:   
55808
GlyMap GeneID:   
55808
DNA aRefSeq:   
NM_018414.4 
Protein RefSeq:   
NP_060884.1 
BRENDA:   
Kinetic info.
OMIM number:   
610138
GeneCards database:   
ST6GALNAC1
Enzyme Specificity
Glycan Type:  
     O-linked (mucin type), O-linked (other)
Compartment:  
     
Substrate:  
     ...GalNAc(a1-?)Ser|Thr
Product:  
     ...[NeuAc(a2-6)]GalNAc(a1-?)Ser|Thr
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 120 100 80 60 40 20 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4515 285-292 -0.529 99.0 -0.529 99.0 noncon
hsa-miR-4305 413-420 -0.52 99.0 -0.52 99.0 noncon
hsa-miR-3619-5p 266-273 -0.481 99.0 -0.481 99.0 noncon
hsa-miR-1269a 286-293 -0.47 99.0 -0.47 99.0 noncon
hsa-miR-214-3p 266-273 -0.459 99.0 -0.459 99.0 noncon
hsa-miR-1269b 286-293 -0.454 98.0 -0.454 99.0 noncon
hsa-miR-6874-5p 98-105 -0.451 98.0 -0.451 99.0 noncon
hsa-miR-3156-3p 52-59 -0.417 98.0 -0.417 99.0 noncon
hsa-miR-1914-5p 41-48 -0.417 98.0 -0.417 99.0 noncon
hsa-miR-1199-5p 87-94 -0.416 98.0 -0.416 99.0 noncon
hsa-miR-6751-3p 87-94 -0.413 98.0 -0.413 99.0 noncon
hsa-miR-6837-3p 562-569 -0.401 98.0 0.0 25.0 noncon
hsa-miR-6073 402-409 -0.399 98.0 -0.399 99.0 noncon
hsa-miR-761 266-273 -0.389 99.0 -0.389 99.0 noncon
hsa-miR-4529-3p 287-294 -0.383 96.0 -0.383 97.0 noncon
hsa-miR-6802-3p 238-244 -0.364 96.0 -0.364 97.0 noncon
hsa-miR-20b-3p 404-411 -0.364 98.0 -0.364 98.0 noncon
hsa-miR-4291 267-274 -0.35 98.0 -0.35 98.0 noncon
hsa-miR-3926 71-78 -0.342 98.0 -0.342 98.0 noncon
hsa-miR-4701-5p 248-255 -0.336 98.0 -0.336 98.0 noncon
hsa-miR-6715b-3p 346-353 -0.321 98.0 -0.321 99.0 noncon
hsa-miR-4774-3p 543-549 -0.312 97.0 -0.312 98.0 noncon
hsa-miR-3912-5p 139-146 -0.312 97.0 -0.312 97.0 noncon
hsa-miR-588 248-255 -0.304 97.0 -0.304 98.0 noncon
hsa-miR-4776-3p 159-166 -0.295 96.0 -0.295 97.0 noncon
hsa-miR-6128 353-360 -0.291 98.0 -0.291 99.0 noncon
hsa-miR-484 316-322 -0.286 98.0 -0.286 98.0 noncon
hsa-miR-3659 276-282 -0.275 94.0 -0.275 96.0 noncon
hsa-miR-1183 558-564 -0.273 97.0 -0.273 98.0 noncon
hsa-miR-6889-3p 40-46 -0.273 94.0 -0.273 96.0 noncon
hsa-miR-4662a-5p 167-173 -0.272 96.0 -0.272 97.0 noncon
hsa-miR-3155a 316-322 -0.272 97.0 -0.272 98.0 noncon
hsa-miR-3155b 316-322 -0.272 97.0 -0.272 98.0 noncon
hsa-miR-6715a-3p 345-352 -0.271 97.0 -0.271 98.0 noncon
hsa-miR-4670-3p 397-404 -0.27 97.0 -0.27 98.0 noncon
hsa-miR-6500-5p 98-104 -0.267 95.0 -0.267 96.0 noncon
hsa-miR-873-5p.2 264-270 -0.267 96.0 -0.267 97.0 noncon
hsa-miR-3686 326-333 -0.266 96.0 -0.266 97.0 noncon
hsa-miR-642b-5p 123-130 -0.258 94.0 -0.258 96.0 noncon
hsa-miR-6077 280-287 -0.255 97.0 -0.255 97.0 noncon
hsa-miR-4633-3p 98-104 -0.255 94.0 -0.255 95.0 noncon
hsa-miR-181b-3p 561-567 -0.252 96.0 -0.063 80.0 noncon
hsa-miR-181b-2-3p 561-567 -0.252 96.0 -0.063 80.0 noncon