GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
GALNT5



General Information
Gene Symbol:   
GALNT5
Protein name:   
Polypeptide N-acetylgalactosaminyltransferase 5
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
CBM13
E.C. No:   
2.4.1.41
Organs:   
Reaction (SNFG):   
+ Ser →
+ UDP
+ Thr →
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• Localized in Golgi where it catalyzes O-GalNAc glycosylation.

Related Pathway
KEGG ORTHOLOGY:   
K00710
KEGG Pathway:   
ko00512  ko01100 
Reactome:   
R-HSA-913709
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q7Z7M9 
NCBI GeneID:   
11227
GlyMap GeneID:   
11227
DNA aRefSeq:   
NM_001329868.1 
Protein RefSeq:   
NP_001316797.1 
BRENDA:   
Kinetic info.
OMIM number:   
615129
GeneCards database:   
GALNT5
Enzyme Specificity
Glycan Type:  
     O-linked (mucin type)
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Ser or Thr
Product:  
     GalNAc(a1-?)Ser or GalNAc(a1-?)Thr
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000259056 ERG USF1 GMEB1 EHF LYL1 MAZ BHLHE40 ATF2 MAFK HOXA4 TP53 E2F1 NR4A1 MNT MAX ZNF83 HOXA5 NKX2-1 IRF1 PRDM6 PRDM6 TAL1 ZNF692 REST
miRNA-GlycoEnzyme Interactome {ref. 9}
GALNT5/hsa-miR-6505-3p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...AUAGUUUCCACAA AGAAGUCA ...
3'   GAAACCUUCUCCA UCUUCAG U
 8mer 2302-2309 -0.322 96.0 -0.064 72.0 noncon