GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
GCNT2



General Information
Gene Symbol:   
GCNT2
Protein name:   
N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT14
E.C. No:   
2.4.1.150
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• This is the β1,6-N-acetylglucosaminyltransferase or IGnT enzyme, forming the GlcNAcβ1-3(GlcNAcβ1-6)Galβ1-3GalNAcα1-R branch from the linear GlcNAcβ1-3Galβ1-3GalNAcα1-R lactosamine structure.
• In humans, GCNT2 is expressed as three spliced variants IGnTA, IGnTB and IGnTC.
• GCNT2 is overexpressed in highly metastatic breast cancer cell lines and basal-like breast tumor samples. Thus, GCNT2 expression correlates with metastatic phenotype of tumors.
• GCNT2 also enhances cell detachment, adhesion to endothelial cells, cell migration and invasion in vitro, and lung metastasis of breast cancer cells in vivo.

Related Pathway
KEGG ORTHOLOGY:   
K00742
KEGG Pathway:   
ko00601  ko01100 
Reactome:   
No Record
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q8N0V5 
NCBI GeneID:   
2651
GlyMap GeneID:   
2651
DNA aRefSeq:   
NM_001491.2 
Protein RefSeq:   
NP_001482.1 
BRENDA:   
Kinetic info.
OMIM number:   
600429
GeneCards database:   
GCNT2
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-4)GlcNAc(b1-3)Gal(b1-4)GlcNAc(b1-?)
Product:  
     Gal(b1-4)GlcNAc(b1-3)[GlcNAc(b1-6)]Gal(b1-4)GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 60 50 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000265012 ENST00000316170 ENST00000379597 ENST00000397423 ENST00000410107 ENST00000474983 ENST00000489225 ENST00000489819 OTX2 TAF1 SMAD4 NANOG OTX2 YY1 SOX2 TAL1 FOXA1 TBP SP1 TEAD4 NANOG ZNF449
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-199a-5p 2527-2534 -0.483 98.0 -0.483 98.0 con
hsa-miR-199b-5p 2527-2534 -0.483 98.0 -0.483 98.0 con
hsa-miR-6763-3p 1431-1438 -0.463 97.0 -0.463 97.0 noncon
hsa-miR-548q 300-307 -0.398 99.0 -0.398 99.0 noncon
hsa-miR-7162-3p 287-294 -0.388 99.0 -0.388 99.0 noncon
hsa-miR-3127-5p 2676-2683 -0.384 97.0 -0.384 98.0 noncon
hsa-miR-7109-5p 1990-1997 -0.367 96.0 -0.367 97.0 noncon
hsa-miR-4255 719-726 -0.362 99.0 -0.362 99.0 noncon
hsa-miR-95-3p 1648-1654 -0.358 92.0 -0.358 94.0 noncon
hsa-miR-1911-3p 919-926 -0.348 97.0 -0.348 98.0 noncon
hsa-miR-135a-5p 1525-1532 -0.347 97.0 -0.347 98.0 con
hsa-miR-135b-5p 1525-1532 -0.347 97.0 -0.347 98.0 con
hsa-miR-1294 1347-1354 -0.343 97.0 -0.343 98.0 noncon
hsa-miR-3940-3p 2270-2276 -0.339 92.0 -0.339 94.0 noncon
hsa-miR-4725-5p 2449-2456 -0.328 95.0 -0.328 97.0 noncon
hsa-miR-124-5p 1716-1723 -0.316 95.0 -0.316 96.0 noncon
hsa-miR-5586-3p 1096-1103 -0.305 97.0 -0.305 98.0 noncon
hsa-miR-5588-5p 676-683 -0.304 97.0 -0.304 98.0 noncon
hsa-miR-4792 238-244 -0.301 95.0 -0.301 96.0 noncon
hsa-miR-3681-3p 158-165 -0.301 97.0 -0.301 98.0 con
hsa-miR-216a-3p 158-165 -0.299 97.0 -0.299 98.0 con
hsa-miR-1295b-5p 458-465 -0.299 98.0 -0.299 99.0 noncon
hsa-miR-6764-3p 1541-1548 -0.299 98.0 -0.299 98.0 noncon
hsa-miR-6879-3p 2390-2397 -0.296 91.0 -0.296 94.0 noncon
hsa-miR-887-3p 1004-1010 -0.295 93.0 -0.295 95.0 noncon
hsa-miR-486-5p 1052-1059 -0.295 97.0 -0.295 98.0 noncon
hsa-miR-4720-5p 676-683 -0.291 97.0 -0.291 98.0 noncon
hsa-miR-4799-3p 676-683 -0.288 97.0 -0.288 98.0 noncon
hsa-miR-1267 1257-1264 -0.287 97.0 -0.287 98.0 noncon
hsa-miR-302f 1685-1692 -0.287 97.0 -0.287 98.0 noncon
hsa-miR-1273g-3p 2457-2464 -0.287 97.0 -0.287 98.0 noncon
hsa-miR-1912 458-465 -0.286 98.0 -0.286 98.0 noncon
hsa-miR-4727-3p 1926-1933 -0.283 91.0 -0.283 94.0 noncon
hsa-miR-128-3p 158-165 -0.283 97.0 -0.283 98.0 con
hsa-miR-6824-3p 1541-1548 -0.283 97.0 -0.283 98.0 noncon
hsa-miR-7977 39-46 -0.274 97.0 -0.274 98.0 noncon
hsa-miR-7109-3p 1408-1415 -0.269 96.0 -0.269 97.0 noncon
hsa-miR-4696 220-226 -0.267 96.0 -0.267 97.0 noncon
hsa-miR-7978 1125-1132 -0.266 96.0 -0.266 97.0 noncon
hsa-miR-6892-3p 1263-1270 -0.265 93.0 -0.265 95.0 noncon
hsa-miR-7-5p 707-714 -0.265 96.0 -0.265 97.0 noncon
hsa-miR-199b-3p 2684-2690 -0.262 93.0 -0.262 95.0 con
hsa-miR-199a-3p 2684-2690 -0.262 93.0 -0.262 95.0 con
hsa-miR-4741 2661-2668 -0.261 96.0 -0.261 97.0 noncon
hsa-miR-3591-5p 721-728 -0.259 95.0 -0.259 96.0 noncon
hsa-miR-384 361-368 -0.258 97.0 -0.258 98.0 noncon
hsa-miR-626 2499-2505 -0.257 90.0 -0.257 93.0 noncon
hsa-miR-6126 1019-1026 -0.251 97.0 -0.251 98.0 noncon
hsa-miR-4786-5p 1565-1572 -0.25 94.0 -0.25 96.0 noncon