GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
UGCG



General Information
Gene Symbol:   
UGCG
Protein name:   
Ceramide glucosyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT21
E.C. No:   
2.4.1.80
Organs:   
Reaction (SNFG):   
+ Cer →
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• UGCG (GlcT-1/GCS/CEGT/UGCG) transfers Glc from UDP-Glc to ceramide (Cer) to generate GlcCer
• GlcCer is synthesized at cytosolic side of Golgi membrane before it is flipped to the non-cytoplasmic face by a lipid flippase.
• Mouse lacking this enzyme is embryonic lethal at day 8.
• Common inhibitors of this enzyme activity include D-threo-PDMP ([(R, R)-1-phenyl-2-decanoylamino-3-morpholino-1-propanol]), N-butyldeoxynojirimycin (NB-DNJ), and N-(5-adamantane-1-s-pentyl)-deoxynojirimycin (AMP-DNM).
• NB-DNJ (miglusat) is used as oral drug to treat for patients with a type of Gaucher disease.
• Other pathogenesis involving UGCG include diabetes mellitus, atherosclerosis and polycystic kidney.

Related Pathway
KEGG ORTHOLOGY:   
K00720
KEGG Pathway:   
ko00600  ko01100 
Reactome:   
R-HSA-1660662
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q16739 
NCBI GeneID:   
7357
GlyMap GeneID:   
7357
DNA aRefSeq:   
NM_003358.2 
Protein RefSeq:   
NP_003349.1 
BRENDA:   
Kinetic info.
OMIM number:   
602874
GeneCards database:   
UGCG
Enzyme Specificity
Glycan Type:  
     GSL
Compartment:  
     
Substrate:  
     Cer
Product:  
     Glc(b1-r):Cer
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 50 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 300 200 100 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
UGCG/hsa-miR-24-3p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...ACUUUUUCACUUUA CUGAGCCA ...
3'   GACAAGGACGACUU GACUCGG U
 8mer 1600-1607 -0.507 99.0 -0.488 99.0 con