GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
CHST5



General Information
Gene Symbol:   
CHST5
Protein name:   
Carbohydrate sulfotransferase 5
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.*
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Five GlcNAc6ST enzymes have been found in humans.
• GlcNAc6ST-1 (CHST2) catalyzes the formation of 6-sulfo sialyl Lewis-X (6-sulfo sLeX, the major L-selectin ligand on HEV. This is also a major enzyme facilitating C6-GlcNAc sulfation of KS. CHST2 acts at the non-reducing end of GlcNAc(β1-3)Gal(β1-4)GlcNAc(β1-3).
• GlcNAc6ST-2 (CHST4) is expressed on High Endothelial Cells of the lymph node (HEV). Both CHST2 and CHST4 can make 6-sulfo sialyl Lewis-X. It is also involved in the biosynthesis of MECA-79 epitope. CHST4 acts internally on LacNAc substrate.
• GlcNAc6ST-3 (CHST5) is a GlcNAc6ST that is functional in the intestines. It is highly expressed in colon and small intestine. CHST5 does not act to form KS, but it may attach sulfate to C6 on O-glycan core-2 glycan at the non-reducing end. As it is located in the intestines, it does not participate in the formation of the sulfated sLeX epitope.
• GlcNAc6ST-4/GST-5 (CHST7) is widely expressed in many organs. Less is known about this enzyme. It may contribute to C6 sulfation of GalNAc during chondroitin sulfate biosynthesis.
• GlcNAc6ST-5 (CHST6) is expressed in the cornea, and involved in the sulfation of keratin sulfate GAGs of the eye. The absence of this enzyme results in macular corneal dystrophy.

Related Pathway
KEGG ORTHOLOGY:   
K09670
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-2022854
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9GZS9 
NCBI GeneID:   
23563
GlyMap GeneID:   
23563
DNA aRefSeq:   
NM_024533.4 
Protein RefSeq:   
NP_078809.2 
BRENDA:   
Kinetic info.
OMIM number:   
604817
GeneCards database:   
CHST5
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-3)[GlcNAc(b1-6)]GalNAc(a1-?)Ser|Thr or Gal(b1-4)GlcNAc(b1-3)Gal(b1-?)
Product:  
     Gal(b1-3)[GlcNAc(b1-6 [6S])]GalNAc(a1-?)Ser|Thr or Gal(b1-4)GlcNAc(b1-3 [6S])Gal(b1-?)
Constraints:  
     var=50%
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-1286 611-618 -0.815 99.0 -0.001 37.0 noncon
hsa-miR-6727-5p 22-29 -0.684 98.0 -0.684 99.0 noncon
hsa-miR-1281 48-55 -0.523 98.0 -0.523 99.0 noncon
hsa-miR-1289 503-510 -0.497 98.0 -0.497 99.0 noncon
hsa-miR-24-2-5p 135-142 -0.463 96.0 -0.463 98.0 noncon
hsa-miR-24-1-5p 135-142 -0.463 96.0 -0.463 98.0 noncon
hsa-miR-4688 240-247 -0.457 99.0 -0.457 99.0 noncon
hsa-miR-5007-5p 572-579 -0.454 99.0 -0.454 99.0 noncon
hsa-miR-374b-3p 275-282 -0.449 99.0 -0.449 99.0 noncon
hsa-miR-6743-5p 240-247 -0.443 99.0 -0.443 99.0 noncon
hsa-miR-6804-3p 211-218 -0.441 98.0 -0.441 98.0 noncon
hsa-miR-1915-5p 139-146 -0.432 97.0 -0.432 98.0 noncon
hsa-miR-6787-3p 529-536 -0.407 99.0 -0.407 99.0 noncon
hsa-miR-1227-3p 448-455 -0.398 97.0 -0.398 98.0 noncon
hsa-miR-6732-5p 408-414 -0.371 97.0 -0.371 98.0 noncon
hsa-miR-4435 391-398 -0.362 97.0 -0.362 98.0 noncon
hsa-miR-1539 258-264 -0.358 94.0 -0.358 95.0 noncon
hsa-miR-4768-3p 162-169 -0.357 99.0 -0.357 99.0 noncon
hsa-miR-1224-3p 486-493 -0.355 98.0 -0.355 98.0 noncon
hsa-miR-431-5p 569-575 -0.352 98.0 -0.352 98.0 noncon
hsa-miR-941 303-309 -0.35 91.0 -0.35 95.0 noncon
hsa-miR-6856-5p 412-419 -0.345 99.0 -0.345 99.0 noncon
hsa-miR-3194-3p 564-571 -0.342 97.0 -0.342 98.0 noncon
hsa-miR-6775-5p 22-28 -0.339 90.0 -0.339 93.0 noncon
hsa-miR-5090 22-28 -0.336 90.0 -0.336 92.0 noncon
hsa-miR-6758-5p 412-419 -0.327 98.0 -0.327 99.0 noncon
hsa-miR-4523 171-177 -0.326 95.0 -0.326 97.0 noncon
hsa-miR-6892-3p 66-73 -0.317 96.0 -0.317 97.0 noncon
hsa-miR-4695-3p 532-539 -0.316 97.0 -0.316 97.0 noncon
hsa-miR-562 470-477 -0.312 98.0 -0.312 98.0 noncon
hsa-miR-873-5p.2 612-618 -0.31 98.0 -0.001 16.0 noncon
hsa-miR-3680-3p 426-433 -0.307 98.0 -0.307 98.0 noncon
hsa-miR-532-3p 488-495 -0.303 98.0 -0.303 98.0 noncon
hsa-miR-5095 322-329 -0.293 96.0 -0.293 97.0 noncon
hsa-miR-4644 160-166 -0.292 96.0 -0.292 97.0 noncon
hsa-miR-6811-5p 613-619 -0.289 96.0 -0.001 33.0 noncon
hsa-miR-4306 160-166 -0.281 96.0 -0.281 96.0 noncon
hsa-miR-185-5p 160-166 -0.281 95.0 -0.281 96.0 noncon
hsa-miR-8083 609-615 -0.275 96.0 -0.001 28.0 noncon
hsa-miR-6511b-5p 613-619 -0.273 95.0 -0.001 35.0 noncon
hsa-miR-1234-3p 300-306 -0.272 93.0 -0.272 95.0 noncon
hsa-miR-7151-3p 322-329 -0.266 95.0 -0.266 97.0 noncon
hsa-miR-4755-3p 389-395 -0.266 93.0 -0.266 95.0 noncon
hsa-miR-6789-5p 241-247 -0.256 91.0 -0.256 94.0 noncon
hsa-miR-6770-5p 202-209 -0.253 95.0 -0.253 96.0 noncon
hsa-miR-4274 274-280 -0.252 95.0 -0.252 97.0 noncon
hsa-miR-4722-5p 612-618 -0.25 97.0 -0.001 30.0 noncon
hsa-miR-7107-5p 300-306 -0.25 91.0 -0.25 94.0 noncon