GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
GAL3ST2



General Information
Gene Symbol:   
GAL3ST2
Protein name:   
Galactose-3-O-sulfotransferase 2
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.*
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• There are 4 enzymes that perform 3-O-sulfation of Gal.
• Gal3ST1: Acts on GalCer and LacCer to create sulfated glycolipids. Catalyzes the synthesis of sulfatides and seminolpids.
• Gal3ST2: Galβ1,4GlcNAc of O- and N-glycan, can act on sulfated substrates to form bisulfated carbohydrates like 3′,6-disulfo LewisX and 3′,6'-disulfo LewisX. This can also form 3'-sulfo-Le a and 3-O-sulfo-LeX determinants and it can act on lipid substrates
• Gal3ST3 Galβ1,4GlcNAc of O- and N-glycan and Galβ1,4GalNAc of GloboH structure. Can act on sulfated substrates to form bisulfated carbohydrates
• Gal3ST4 Galβ1,4GalNAc of Globo glycolipids and glycopeptides

Related Pathway
KEGG ORTHOLOGY:   
K09675
KEGG Pathway:   
No Record 
Reactome:   
No Record
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9H3Q3 
NCBI GeneID:   
64090
GlyMap GeneID:   
64090
DNA aRefSeq:   
NM_022134.2 
Protein RefSeq:   
NP_071417.2 
BRENDA:   
Kinetic info.
OMIM number:   
608237
GeneCards database:   
GAL3ST2
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-4)GlcNAc(b1-?) or Gal(b1-4)GlcNAc(b1-? [6S])
Product:  
     Gal(b1-4 [3S])GlcNAc(b1-?) or Gal(b1-4 [3S])GlcNAc(b1-? [6S])
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 10 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 8 6 4 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000192314 ENST00000610560 ENST00000632847 TFAP4 GRHL2 MECOM RUNX1
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6886-5p 26-33 -0.866 99.0 -0.866 99.0 noncon
hsa-miR-4707-5p 66-73 -0.576 99.0 -0.001 20.0 noncon
hsa-miR-6799-3p 79-85 -0.436 98.0 -0.001 31.0 noncon
hsa-miR-138-5p 34-40 -0.405 96.0 -0.405 97.0 noncon
hsa-miR-3173-5p 79-85 -0.403 98.0 -0.001 33.0 noncon
hsa-miR-4669 30-36 -0.385 99.0 -0.385 99.0 noncon
hsa-miR-4487 36-42 -0.381 98.0 -0.381 98.0 noncon
hsa-miR-6779-3p 80-86 -0.333 98.0 -0.001 25.0 noncon
hsa-miR-6763-3p 58-64 -0.31 90.0 -0.001 10.0 noncon
hsa-miR-7978 33-39 -0.292 96.0 -0.292 97.0 noncon
hsa-miR-4743-5p 114-120 -0.29 91.0 -0.001 6.0 noncon
hsa-miR-5739 45-51 -0.288 90.0 -0.001 9.0 noncon
hsa-miR-4695-5p 22-28 -0.267 95.0 -0.267 97.0 noncon
hsa-miR-4707-5p 58-64 -0.264 90.0 -0.001 18.0 noncon
hsa-miR-28-5p 38-44 -0.261 95.0 -0.001 21.0 noncon