GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ALG6



General Information
Gene Symbol:   
ALG6
Protein name:   
Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT57
E.C. No:   
2.4.1.267
Organs:   
Reaction (SNFG):   
+
→
+ P-Dol
Reaction (IUBMB):   
No specified reaction.
About

• Dol-P-Glc is formed from UDP-Glc using ALG5
• Three Glc residues are then transferred from Dol-P-Glc to the LLO using ALG6 [α(1,3)GlcT], ALG8 [α(1,3)GlcT] and ALG10 [α(1,2)GlcT].

Related Pathway
KEGG ORTHOLOGY:   
K03848
KEGG Pathway:   
ko00510  ko01100 
Reactome:   
R-HSA-446193
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9Y672 
NCBI GeneID:   
29929
GlyMap GeneID:   
29929
DNA aRefSeq:   
NM_013339.3 
Protein RefSeq:   
NP_037471.2 
BRENDA:   
Kinetic info.
OMIM number:   
604566
GeneCards database:   
ALG6
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     ER
Substrate:  
     Man(a1-2)Man(a1-2)Man(a1-3)
Product:  
     Glc(a1-3)Man(a1-2)Man(a1-2)Man(a1-3)
Constraints:  
     nMan(a1-2)Man(a1-2)Man(a1-3)[Man(a1-2)Man(a1-6)[Man(a1-2)Man(a1-3)]Man(a1-6)]Man(b1-4)>=1
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4286 249-256 -0.454 99.0 -0.158 91.0 noncon
hsa-miR-597-3p 103-110 -0.452 98.0 -0.441 99.0 noncon
hsa-miR-1265 2799-2806 -0.438 99.0 -0.093 87.0 noncon
hsa-miR-6891-3p 1449-1456 -0.418 98.0 -0.122 86.0 noncon
hsa-miR-203a-5p 113-120 -0.417 98.0 -0.407 98.0 noncon
hsa-miR-24-1-5p 1891-1898 -0.415 95.0 -0.095 70.0 noncon
hsa-miR-24-2-5p 1891-1898 -0.391 94.0 -0.09 69.0 noncon
hsa-miR-1909-3p 2425-2432 -0.386 97.0 -0.089 72.0 noncon
hsa-miR-6072 1449-1456 -0.381 98.0 -0.113 85.0 noncon
hsa-miR-23a-3p 32-39 -0.371 96.0 -0.371 97.0 noncon
hsa-miR-23b-3p 32-39 -0.371 96.0 -0.371 97.0 noncon
hsa-miR-3651 1930-1937 -0.364 96.0 -0.084 77.0 noncon
hsa-miR-6821-3p 2605-2612 -0.359 98.0 -0.078 79.0 noncon
hsa-miR-23c 32-39 -0.355 96.0 -0.355 97.0 noncon
hsa-miR-1200 291-298 -0.354 98.0 -0.126 88.0 noncon
hsa-miR-6779-3p 1928-1935 -0.351 98.0 -0.082 79.0 noncon
hsa-miR-6722-3p 2425-2432 -0.349 96.0 -0.081 71.0 noncon
hsa-miR-584-5p 618-625 -0.347 97.0 -0.124 86.0 noncon
hsa-miR-130a-5p 32-39 -0.337 96.0 -0.337 97.0 noncon
hsa-miR-4312 606-613 -0.333 98.0 -0.119 92.0 noncon
hsa-miR-7976 2278-2285 -0.333 98.0 -0.078 77.0 noncon
hsa-miR-4496 20-27 -0.332 99.0 -0.332 99.0 noncon
hsa-miR-4774-5p 1758-1765 -0.326 96.0 -0.076 75.0 noncon
hsa-miR-3145-5p 1469-1476 -0.326 98.0 -0.092 86.0 noncon
hsa-miR-6843-3p 173-180 -0.326 98.0 -0.304 98.0 noncon
hsa-miR-764 1535-1542 -0.321 99.0 -0.086 92.0 noncon
hsa-miR-6505-3p 490-497 -0.316 96.0 -0.113 83.0 noncon
hsa-miR-511-3p 737-744 -0.314 98.0 -0.113 86.0 noncon
hsa-miR-659-5p 1898-1905 -0.31 95.0 -0.073 71.0 noncon
hsa-miR-4283 406-412 -0.309 94.0 -0.111 80.0 noncon
hsa-miR-4520-3p 2230-2237 -0.307 97.0 -0.072 76.0 noncon
hsa-miR-23a-3p 40-47 -0.306 95.0 -0.306 96.0 noncon
hsa-miR-96-3p 936-943 -0.303 97.0 -0.109 89.0 noncon
hsa-miR-221-3p 2220-2227 -0.302 95.0 -0.071 72.0 con
hsa-miR-222-3p 2220-2227 -0.302 95.0 -0.071 72.0 con
hsa-miR-23b-3p 40-47 -0.298 95.0 -0.298 96.0 noncon
hsa-miR-23c 40-47 -0.298 95.0 -0.298 96.0 noncon
hsa-miR-4535 1899-1906 -0.297 96.0 -0.07 78.0 noncon
hsa-miR-383-3p 49-56 -0.295 98.0 -0.288 98.0 noncon
hsa-miR-4666b 584-591 -0.294 96.0 -0.106 85.0 noncon
hsa-miR-155-5p 2635-2642 -0.293 95.0 -0.065 73.0 con
hsa-miR-6868-5p 833-840 -0.292 96.0 -0.105 81.0 noncon
hsa-miR-924 1249-1256 -0.288 96.0 -0.087 81.0 noncon
hsa-miR-6806-3p 388-395 -0.288 96.0 -0.104 88.0 noncon
hsa-miR-6857-5p 2213-2220 -0.288 97.0 -0.068 77.0 noncon
hsa-miR-8062 427-434 -0.286 98.0 -0.103 90.0 noncon
hsa-miR-1915-5p 1895-1901 -0.283 92.0 -0.067 70.0 noncon
hsa-miR-27a-5p 2026-2032 -0.282 94.0 -0.067 62.0 noncon
hsa-miR-6734-5p 2442-2448 -0.281 92.0 -0.067 68.0 noncon
hsa-miR-3928-5p 388-395 -0.28 96.0 -0.101 88.0 noncon
hsa-miR-491-3p 1243-1250 -0.28 93.0 -0.085 74.0 noncon
hsa-miR-130a-5p 40-47 -0.28 94.0 -0.28 96.0 noncon
hsa-miR-4450 2212-2219 -0.275 91.0 -0.065 62.0 noncon
hsa-miR-6864-5p 2030-2037 -0.273 95.0 -0.065 75.0 noncon
hsa-miR-1271-3p 1775-1782 -0.268 91.0 -0.064 64.0 noncon
hsa-miR-6732-3p 822-829 -0.267 96.0 -0.097 82.0 noncon
hsa-miR-3672 2370-2377 -0.266 97.0 -0.063 82.0 noncon
hsa-miR-4490 1759-1765 -0.264 93.0 -0.063 64.0 noncon
hsa-miR-6848-3p 173-180 -0.264 98.0 -0.247 98.0 noncon
hsa-miR-586 643-650 -0.264 96.0 -0.096 87.0 noncon
hsa-miR-5010-3p 153-160 -0.262 97.0 -0.256 98.0 noncon
hsa-miR-6815-3p 894-901 -0.262 93.0 -0.095 78.0 noncon
hsa-miR-218-5p 2641-2647 -0.262 93.0 -0.058 74.0 con
hsa-miR-509-3p 2676-2682 -0.261 91.0 -0.058 63.0 noncon
hsa-miR-550a-3-5p 1775-1782 -0.26 91.0 -0.062 64.0 noncon
hsa-miR-550a-5p 1775-1782 -0.26 91.0 -0.062 64.0 noncon
hsa-miR-152-5p 764-771 -0.259 93.0 -0.094 74.0 noncon
hsa-miR-218-1-3p 112-118 -0.259 93.0 -0.253 94.0 noncon
hsa-miR-3158-3p 2027-2033 -0.258 95.0 -0.062 72.0 noncon
hsa-miR-215-5p 2607-2613 -0.255 96.0 -0.057 72.0 con
hsa-miR-6813-5p 407-413 -0.254 93.0 -0.092 78.0 noncon
hsa-miR-3179 409-415 -0.253 92.0 -0.092 76.0 noncon
hsa-miR-4797-3p 535-542 -0.253 97.0 -0.092 84.0 noncon
hsa-miR-192-5p 2607-2613 -0.251 96.0 -0.056 75.0 con
hsa-miR-3938 504-510 -0.25 94.0 -0.091 81.0 noncon