GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ALG2



General Information
Gene Symbol:   
ALG2
Protein name:   
Alpha-1,3/1,6-mannosyltransferase ALG2
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT4
E.C. No:   
2.4.1.132
Organs:   
Reaction (SNFG):   
+
→
+ GDP
+
→
+ GDP
Reaction (IUBMB):   
No specified reaction.
About

• These enzymes sequentially add 5-mannose from GDP-man to generate Man5Glc2-PP-Dol.
• Alg1 is a β1-4Mannosyltransferase.
• Alg2 adds both a(1-3) and a(1-6) Mannose to Man1GlcNAc2-PP-Dol.
• Alg1, Alg2, and Alg11 mannosyltransferases are ER membrane proteins with catalytic domains that face the cytosol and that use GDP-man as the sugar donor for mannosylation.
• Deficiencies in Alg1, Alg2, and Alg11 result in CDG-Ik, CDG-Ii and CDG-Ip, respectively.
• Intermediate glycan built on dolichol phosphate containing 7 monosaccahrides is flipped into the ER lumen. This is further built with 4 Man and 3 Glc.

Related Pathway
KEGG ORTHOLOGY:   
K03843
KEGG Pathway:   
ko00510  ko00513  ko01100 
Reactome:   
R-HSA-446193
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9H553 
NCBI GeneID:   
85365
GlyMap GeneID:   
85365
DNA aRefSeq:   
NM_033087.3 
Protein RefSeq:   
NP_149078.1 
BRENDA:   
Kinetic info.
OMIM number:   
607905
GeneCards database:   
ALG2
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     ER
Substrate:  
     Man(b1-4)GlcNAc(b1-4)GlcNAc(a1-?)Dol-PP or Man(a1-3)Man(b1-4)GlcNAc(b1-4)GlcNAc(a1-?)Dol-PP
Product:  
     Man(a1-3)Man(b1-4)GlcNAc(b1-4)GlcNAc(a1-?)Dol-PP or Man(a1-3)[Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc(a1-?)Dol-PP
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 60 50 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4740-3p 274-281 -0.709 99.0 -0.616 99.0 noncon
hsa-miR-4683 16-23 -0.689 99.0 -0.689 99.0 noncon
hsa-miR-124-3p.1 318-325 -0.609 99.0 -0.53 99.0 con
hsa-miR-29b-2-5p 59-66 -0.517 99.0 -0.517 99.0 noncon
hsa-miR-4480 1242-1249 -0.514 99.0 -0.021 59.0 noncon
hsa-miR-20a-3p 85-92 -0.506 99.0 -0.506 99.0 noncon
hsa-miR-19b-3p 529-536 -0.467 99.0 -0.407 98.0 con
hsa-miR-19a-3p 529-536 -0.467 99.0 -0.407 98.0 con
hsa-miR-494-5p 631-638 -0.462 98.0 -0.02 45.0 noncon
hsa-miR-410-5p 631-638 -0.462 98.0 -0.02 43.0 noncon
hsa-miR-4786-3p 374-381 -0.456 99.0 -0.398 99.0 noncon
hsa-miR-93-3p 249-256 -0.456 99.0 -0.401 99.0 noncon
hsa-miR-603 344-351 -0.456 99.0 -0.398 99.0 noncon
hsa-miR-323b-5p 631-638 -0.454 98.0 -0.019 44.0 noncon
hsa-miR-4471 408-415 -0.45 98.0 -0.393 98.0 noncon
hsa-miR-4284 128-135 -0.45 99.0 -0.396 99.0 noncon
hsa-miR-6779-3p 396-403 -0.449 99.0 -0.392 99.0 noncon
hsa-miR-4673 476-483 -0.448 99.0 -0.391 99.0 noncon
hsa-miR-96-5p 1459-1466 -0.445 98.0 -0.019 57.0 noncon
hsa-miR-4646-5p 410-417 -0.445 99.0 -0.388 99.0 noncon
hsa-miR-4645-5p 476-483 -0.442 99.0 -0.386 99.0 noncon
hsa-miR-376a-5p 79-86 -0.437 99.0 -0.437 99.0 noncon
hsa-miR-4302 533-540 -0.433 99.0 -0.378 99.0 noncon
hsa-miR-1271-5p 1459-1466 -0.426 98.0 -0.018 57.0 noncon
hsa-miR-124-3p.2 319-326 -0.425 97.0 -0.371 96.0 con
hsa-miR-5589-3p 1387-1394 -0.419 99.0 -0.018 48.0 noncon
hsa-miR-150-3p 1429-1436 -0.412 97.0 -0.018 35.0 noncon
hsa-miR-382-3p 308-315 -0.39 99.0 -0.344 99.0 noncon
hsa-miR-28-5p 784-791 -0.387 98.0 -0.017 43.0 noncon
hsa-miR-8059 408-415 -0.382 98.0 -0.334 98.0 noncon
hsa-miR-5089-3p 1041-1048 -0.376 97.0 -0.016 39.0 noncon
hsa-miR-708-5p 784-791 -0.371 98.0 -0.016 43.0 noncon
hsa-miR-3139 784-791 -0.369 98.0 -0.016 43.0 noncon
hsa-miR-506-3p 319-326 -0.365 97.0 -0.32 97.0 con
hsa-miR-141-3p 214-221 -0.364 99.0 -0.321 98.0 con
hsa-miR-200a-3p 214-221 -0.364 99.0 -0.321 99.0 con
hsa-miR-202-3p 1141-1148 -0.361 97.0 -0.016 36.0 noncon
hsa-miR-204-3p 410-417 -0.359 99.0 -0.315 99.0 noncon
hsa-miR-3138 159-165 -0.353 94.0 -0.312 94.0 noncon
hsa-miR-648 532-538 -0.35 98.0 -0.307 98.0 noncon
hsa-miR-1225-3p 1348-1354 -0.349 96.0 -0.015 48.0 noncon
hsa-miR-3173-5p 395-401 -0.345 97.0 -0.303 97.0 noncon
hsa-miR-3944-5p 1324-1330 -0.344 97.0 -0.015 61.0 noncon
hsa-miR-3128 210-216 -0.344 98.0 -0.304 98.0 noncon
hsa-miR-6733-3p 928-935 -0.339 98.0 -0.015 62.0 noncon
hsa-miR-6851-5p 151-157 -0.329 96.0 -0.291 95.0 noncon
hsa-miR-892c-3p 572-579 -0.325 98.0 -0.02 59.0 noncon
hsa-miR-5094 930-937 -0.325 98.0 -0.014 47.0 noncon
hsa-miR-6799-3p 395-401 -0.323 96.0 -0.283 96.0 noncon
hsa-miR-6865-3p 1455-1461 -0.318 96.0 -0.014 55.0 noncon
hsa-miR-4676-3p 572-579 -0.317 98.0 -0.019 59.0 noncon
hsa-miR-3651 398-404 -0.315 94.0 -0.276 95.0 noncon
hsa-miR-4432 1473-1479 -0.312 98.0 -0.012 62.0 noncon
hsa-miR-107 1322-1329 -0.307 96.0 -0.014 41.0 noncon
hsa-miR-4432 117-123 -0.307 98.0 -0.307 99.0 noncon
hsa-miR-103a-3p 1322-1329 -0.307 96.0 -0.014 41.0 noncon
hsa-miR-452-5p 572-579 -0.306 97.0 -0.019 56.0 noncon
hsa-miR-1321 153-159 -0.306 97.0 -0.271 96.0 noncon
hsa-miR-7843-3p 375-381 -0.305 96.0 -0.268 96.0 noncon
hsa-miR-5582-5p 317-323 -0.3 97.0 -0.265 96.0 noncon
hsa-miR-5699-5p 1346-1352 -0.299 97.0 -0.013 33.0 noncon
hsa-miR-1270 17-23 -0.298 97.0 -0.298 98.0 noncon
hsa-miR-3618 44-50 -0.298 90.0 -0.298 94.0 noncon
hsa-miR-6757-3p 213-219 -0.294 98.0 -0.26 98.0 noncon
hsa-miR-620 17-23 -0.291 97.0 -0.291 98.0 noncon
hsa-miR-6786-5p 424-430 -0.288 90.0 -0.253 90.0 noncon
hsa-miR-6754-5p 154-160 -0.285 97.0 -0.252 96.0 noncon
hsa-miR-6515-5p 902-908 -0.284 94.0 -0.013 43.0 noncon
hsa-miR-599 355-362 -0.281 98.0 -0.247 97.0 noncon
hsa-miR-4700-3p 466-472 -0.28 97.0 -0.246 97.0 noncon
hsa-miR-3938 451-457 -0.279 95.0 -0.245 95.0 noncon
hsa-miR-1298-3p 412-418 -0.279 95.0 -0.245 95.0 noncon
hsa-miR-4755-3p 828-834 -0.279 94.0 -0.013 48.0 noncon
hsa-miR-5000-3p 465-471 -0.278 96.0 -0.244 95.0 noncon
hsa-miR-4441 154-160 -0.278 96.0 -0.246 96.0 noncon
hsa-miR-4802-3p 242-248 -0.278 97.0 -0.246 97.0 noncon
hsa-miR-548o-3p 53-60 -0.278 99.0 -0.278 99.0 noncon
hsa-miR-6733-3p 945-952 -0.277 96.0 -0.012 60.0 noncon
hsa-miR-4739 153-159 -0.277 95.0 -0.245 94.0 noncon
hsa-miR-4270 154-160 -0.277 96.0 -0.245 96.0 noncon
hsa-miR-544a 87-93 -0.274 96.0 -0.274 97.0 noncon
hsa-miR-6071 250-256 -0.269 96.0 -0.238 96.0 noncon
hsa-miR-593-5p 1458-1464 -0.268 90.0 -0.012 28.0 noncon
hsa-miR-4323 1347-1353 -0.268 95.0 -0.012 32.0 noncon
hsa-miR-2054 1224-1231 -0.266 98.0 -0.012 65.0 noncon
hsa-miR-3156-5p 95-101 -0.266 97.0 -0.266 97.0 noncon
hsa-miR-4645-3p 438-444 -0.266 94.0 -0.234 95.0 noncon
hsa-miR-200c-3p 575-582 -0.265 97.0 -0.016 64.0 noncon
hsa-miR-200b-3p 575-582 -0.265 97.0 -0.016 64.0 noncon
hsa-miR-1468-3p 1275-1282 -0.265 98.0 -0.012 53.0 noncon
hsa-miR-5195-3p 639-646 -0.263 93.0 -0.012 44.0 noncon
hsa-miR-4687-3p 1107-1113 -0.261 94.0 -0.012 43.0 noncon
hsa-miR-216b-3p 343-349 -0.261 97.0 -0.23 96.0 noncon
hsa-miR-4255 651-658 -0.26 96.0 -0.012 62.0 noncon
hsa-miR-3941 345-351 -0.259 97.0 -0.228 97.0 noncon
hsa-miR-219a-1-3p 1077-1083 -0.255 93.0 -0.012 31.0 noncon
hsa-miR-548g-3p 1378-1385 -0.254 98.0 -0.012 64.0 noncon
hsa-miR-1285-5p 1166-1173 -0.253 98.0 -0.012 76.0 noncon
hsa-miR-215-5p 744-750 -0.251 96.0 -0.011 41.0 noncon