GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
DDOST



General Information
Gene Symbol:   
DDOST
Protein name:   
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
Enzyme Class:   
CAZy Family:   
E.C. No:   
Organs:   
Reaction (SNFG):   

No reaction →
Reaction (IUBMB):   
No specified reaction.
About

• A multi-membrane protein ER membrane associated protein complex that mediates the en-bloc transfer of 14 sugar LLO to N-X-S/T motif in nascent polypeptide
• STT3 is the catalytic core of this complex
• Stt3 forms a complex with the accessory membrane proteins DDOST/OST48, RPN1/ribophorin I, RPN2/ribophorin II, DAD1, Ost4, TUSC3/N33 and MGAT1/IAP in mammals for maximal ativity.
• There are two STT3 genes: STT3A and STT3B. Human Stt3A and Stt3B have redundant functions in the co-translational N-glycosylation. Stt3B also mediates the post-translational N-glycosylation after some time, rather than just at the time when the protein enters the ER from the ribosome.
• Glc[0-3]Man[0-9]GlcNAc2β1-PP-Dol + Asn-Xaa-Ser/Thr -> Dol-PP + Glc[0-3]Man[0-9]GlcNAc2β1-Asn-Xaa-Ser/Thr.

Related Pathway
KEGG ORTHOLOGY:   
K12670
KEGG Pathway:   
ko00510  ko00513  ko01100  ko04141 
Reactome:   
R-HSA-1799339
Related Database
Organism:   
Homo sapiens
Uniprot:   
P39656 
NCBI GeneID:   
1650
GlyMap GeneID:   
1650
DNA aRefSeq:   
NM_005216.4 
Protein RefSeq:   
NP_005207.2 
BRENDA:   
Kinetic info.
OMIM number:   
602202
GeneCards database:   
DDOST
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     ER
Substrate:  
     No reaction
Product:  
     
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 140 120 100 80 60 40 20 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 600 500 400 300 200 100 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-3616-3p 593-600 -0.702 99.0 -0.024 36.0 noncon
hsa-miR-1226-5p 594-601 -0.65 99.0 -0.003 43.0 noncon
hsa-miR-532-3p 117-124 -0.57 99.0 -0.57 99.0 con
hsa-miR-4733-5p 153-160 -0.533 99.0 -0.533 99.0 noncon
hsa-miR-1915-5p 36-43 -0.505 98.0 -0.505 99.0 noncon
hsa-miR-4766-3p 193-200 -0.481 99.0 -0.024 58.0 noncon
hsa-miR-4524a-3p 530-537 -0.456 99.0 -0.017 53.0 noncon
hsa-miR-520g-3p 185-192 -0.453 99.0 -0.453 99.0 noncon
hsa-miR-520h 185-192 -0.453 99.0 -0.453 99.0 noncon
hsa-miR-8057 135-142 -0.433 99.0 -0.433 99.0 noncon
hsa-miR-4790-5p 581-587 -0.425 95.0 -0.016 35.0 noncon
hsa-miR-6776-3p 61-67 -0.391 98.0 -0.391 99.0 noncon
hsa-miR-6887-3p 41-47 -0.376 95.0 -0.376 96.0 noncon
hsa-miR-6790-3p 318-325 -0.368 98.0 -0.014 50.0 noncon
hsa-miR-2682-3p 461-468 -0.353 98.0 -0.014 49.0 noncon
hsa-miR-6781-3p 461-468 -0.34 98.0 -0.013 49.0 noncon
hsa-miR-6746-3p 309-315 -0.339 90.0 -0.013 22.0 noncon
hsa-miR-504-3p 499-506 -0.331 97.0 -0.013 60.0 noncon
hsa-miR-125a-3p 605-611 -0.326 98.0 0.0 0.0 noncon
hsa-miR-1976 440-447 -0.319 97.0 -0.013 68.0 noncon
hsa-miR-532-3p 146-152 -0.318 98.0 -0.318 98.0 noncon
hsa-miR-1227-3p 99-105 -0.312 94.0 -0.312 96.0 noncon
hsa-miR-6747-3p 438-445 -0.301 97.0 -0.012 49.0 noncon
hsa-miR-212-5p 138-144 -0.298 96.0 -0.298 97.0 noncon
hsa-miR-1236-5p 224-230 -0.295 95.0 -0.012 54.0 noncon
hsa-miR-6787-3p 481-488 -0.291 98.0 -0.012 62.0 noncon
hsa-miR-552-3p 408-415 -0.29 96.0 -0.012 43.0 noncon
hsa-miR-4257 111-117 -0.288 98.0 -0.288 98.0 noncon
hsa-miR-4667-3p 40-46 -0.284 96.0 -0.284 97.0 noncon
hsa-miR-662 89-95 -0.283 91.0 -0.283 94.0 noncon
hsa-miR-5095 275-282 -0.277 95.0 -0.011 58.0 noncon
hsa-miR-4712-3p 231-237 -0.273 97.0 -0.011 49.0 noncon
hsa-miR-6822-3p 135-141 -0.27 95.0 -0.27 96.0 noncon
hsa-miR-3118 222-228 -0.267 93.0 -0.011 40.0 noncon
hsa-miR-196a-3p 590-596 -0.265 92.0 -0.011 34.0 noncon
hsa-miR-1260b 145-151 -0.264 93.0 -0.264 95.0 noncon
hsa-miR-1260a 145-151 -0.264 93.0 -0.264 95.0 noncon
hsa-miR-4713-5p 118-124 -0.263 95.0 -0.263 96.0 noncon
hsa-miR-4722-3p 439-445 -0.26 96.0 -0.01 37.0 noncon
hsa-miR-7151-3p 275-282 -0.258 95.0 -0.01 59.0 noncon
hsa-miR-1224-3p 145-151 -0.257 95.0 -0.257 97.0 noncon
hsa-miR-6841-3p 37-43 -0.256 91.0 -0.256 94.0 noncon
hsa-miR-4721 594-600 -0.254 91.0 -0.01 29.0 noncon
hsa-miR-141-3p 517-524 -0.253 96.0 -0.01 53.0 noncon