GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ALG10



General Information
Gene Symbol:   
ALG10
Protein name:   
Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT59
E.C. No:   
2.4.1.256
Organs:   
Reaction (SNFG):   
+
→
+ P-Dol
Reaction (IUBMB):   
No specified reaction.
About

• Dol-P-Glc is formed from UDP-Glc using ALG5
• Three Glc residues are then transferred from Dol-P-Glc to the LLO using ER-resident ALG6 [α(1,3)GlcT], ALG8 [α(1,3)GlcT] and ALG10 [α(1,2)GlcT].

Related Pathway
KEGG ORTHOLOGY:   
K03850
KEGG Pathway:   
ko00510  ko01100 
Reactome:   
R-HSA-446193
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q5BKT4 
NCBI GeneID:   
84920
GlyMap GeneID:   
84920
DNA aRefSeq:   
NM_032834.3 
Protein RefSeq:   
NP_116223.3 
BRENDA:   
Kinetic info.
OMIM number:   
-
GeneCards database:   
ALG10
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     ER
Substrate:  
     Glc(a1-3)Glc(a1-3)Man(a1-2)Man(a1-2)Man(a1-3)
Product:  
     Glc(a1-2)Glc(a1-3)Glc(a1-3)Man(a1-2)Man(a1-2)Man(a1-3)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6503-3p 1048-1055 -0.604 99.0 -0.407 99.0 noncon
hsa-miR-4529-5p 667-674 -0.594 99.0 -0.401 98.0 noncon
hsa-miR-4708-5p 1275-1282 -0.586 99.0 -0.396 99.0 noncon
hsa-miR-1537-3p 2431-2438 -0.576 98.0 0.0 8.0 noncon
hsa-miR-383-5p.2 18-25 -0.566 99.0 -0.566 99.0 noncon
hsa-miR-5088-5p 982-989 -0.555 99.0 -0.376 98.0 noncon
hsa-miR-670-3p 38-45 -0.553 99.0 -0.553 99.0 noncon
hsa-miR-223-5p 1409-1416 -0.552 99.0 -0.375 98.0 noncon
hsa-miR-4537 180-187 -0.544 98.0 -0.369 95.0 noncon
hsa-miR-3190-3p 500-507 -0.524 99.0 -0.357 98.0 noncon
hsa-miR-4279 130-137 -0.524 99.0 -0.357 98.0 noncon
hsa-miR-4480 726-733 -0.52 99.0 -0.354 98.0 noncon
hsa-miR-3171 1077-1084 -0.511 99.0 -0.348 98.0 noncon
hsa-miR-517-5p 1114-1121 -0.51 99.0 -0.348 98.0 noncon
hsa-miR-1226-3p 678-685 -0.502 99.0 -0.343 98.0 noncon
hsa-miR-425-5p 1033-1040 -0.502 99.0 -0.343 99.0 noncon
hsa-miR-942-3p 553-560 -0.492 99.0 -0.336 98.0 noncon
hsa-miR-4256 5088-5095 -0.483 99.0 0.0 24.0 noncon
hsa-miR-6802-5p 1530-1537 -0.479 98.0 0.0 12.0 noncon
hsa-miR-3136-5p 5577-5584 -0.477 99.0 0.0 17.0 noncon
hsa-miR-4763-3p 1917-1924 -0.473 99.0 0.0 25.0 noncon
hsa-miR-1207-5p 1917-1924 -0.455 99.0 0.0 27.0 noncon
hsa-miR-4704-5p 1387-1394 -0.455 99.0 -0.312 99.0 noncon
hsa-miR-6873-5p 4371-4378 -0.443 99.0 0.0 14.0 noncon
hsa-miR-3132 50-56 -0.443 97.0 -0.443 98.0 noncon
hsa-miR-6749-3p 3322-3329 -0.428 98.0 0.0 26.0 noncon
hsa-miR-7161-3p 1043-1050 -0.42 99.0 -0.289 98.0 noncon
hsa-miR-1827 3580-3587 -0.418 99.0 0.0 18.0 noncon
hsa-miR-939-3p 3995-4002 -0.418 98.0 0.0 38.0 noncon
hsa-miR-1827 223-230 -0.417 99.0 -0.287 96.0 noncon
hsa-miR-6817-3p 481-488 -0.413 99.0 -0.285 98.0 noncon
hsa-miR-4736 258-265 -0.407 99.0 -0.281 98.0 noncon
hsa-miR-6755-5p 51-57 -0.404 97.0 -0.404 98.0 noncon
hsa-miR-770-5p 3708-3715 -0.404 98.0 0.0 25.0 noncon
hsa-miR-645 1384-1390 -0.397 97.0 -0.274 94.0 noncon
hsa-miR-4712-5p 3708-3715 -0.396 98.0 0.0 25.0 noncon
hsa-miR-7154-5p 894-901 -0.392 99.0 -0.271 97.0 noncon
hsa-miR-548an 747-754 -0.389 99.0 -0.269 98.0 noncon
hsa-miR-4439 5577-5584 -0.388 99.0 0.0 24.0 noncon
hsa-miR-197-5p 50-56 -0.385 95.0 -0.385 96.0 noncon
hsa-miR-200c-5p 3790-3797 -0.384 98.0 0.0 24.0 noncon
hsa-miR-4724-5p 1069-1076 -0.381 99.0 -0.264 98.0 noncon
hsa-miR-4773 91-98 -0.38 99.0 -0.263 98.0 noncon
hsa-miR-4754 3510-3516 -0.375 92.0 0.0 3.0 noncon
hsa-miR-3613-3p 5412-5418 -0.37 99.0 0.0 28.0 noncon
hsa-miR-499b-3p 1681-1688 -0.369 99.0 0.0 38.0 noncon
hsa-miR-889-5p 5254-5261 -0.368 98.0 0.0 22.0 noncon
hsa-miR-31-3p 1352-1358 -0.363 96.0 -0.252 95.0 noncon
hsa-miR-6779-5p 368-375 -0.362 97.0 -0.251 94.0 noncon
hsa-miR-591 4180-4187 -0.361 96.0 0.0 13.0 noncon
hsa-miR-618 1332-1339 -0.36 99.0 -0.25 98.0 noncon
hsa-miR-1343-3p 3996-4003 -0.358 98.0 0.0 27.0 noncon
hsa-miR-649 2574-2581 -0.357 98.0 0.0 24.0 noncon
hsa-miR-5682 567-573 -0.355 97.0 -0.246 95.0 noncon
hsa-miR-550a-3p 3790-3797 -0.355 97.0 0.0 23.0 noncon
hsa-miR-496.2 1074-1081 -0.355 98.0 -0.246 97.0 noncon
hsa-miR-4309 4960-4967 -0.355 98.0 0.0 45.0 noncon
hsa-miR-6132 962-968 -0.354 96.0 -0.245 93.0 noncon
hsa-miR-499a-3p 1681-1688 -0.353 98.0 0.0 38.0 noncon
hsa-miR-4657 502-508 -0.352 98.0 -0.244 96.0 noncon
hsa-miR-202-3p 5182-5189 -0.352 96.0 0.0 11.0 noncon
hsa-miR-539-3p 5458-5465 -0.351 96.0 0.0 11.0 noncon
hsa-miR-490-5p 2801-2808 -0.346 98.0 0.0 26.0 noncon
hsa-miR-6783-3p 3996-4003 -0.345 98.0 0.0 25.0 noncon
hsa-let-7f-1-3p 1374-1381 -0.343 99.0 -0.238 98.0 noncon
hsa-miR-1244 4243-4250 -0.342 97.0 0.0 17.0 noncon
hsa-miR-146a-3p 479-486 -0.34 98.0 -0.236 97.0 noncon
hsa-miR-4650-3p 49-55 -0.336 95.0 -0.336 96.0 noncon
hsa-miR-505-3p.1 2710-2717 -0.335 94.0 0.0 7.0 noncon
hsa-miR-4742-5p 3904-3911 -0.333 98.0 0.0 24.0 noncon
hsa-miR-6780a-5p 368-375 -0.333 97.0 -0.231 93.0 noncon
hsa-miR-6873-3p 482-489 -0.331 97.0 -0.23 95.0 noncon
hsa-miR-3689c 368-375 -0.33 96.0 -0.229 93.0 noncon
hsa-miR-3689a-3p 368-375 -0.33 96.0 -0.229 94.0 noncon
hsa-miR-1273h-5p 368-375 -0.33 96.0 -0.229 93.0 noncon
hsa-miR-3689b-3p 368-375 -0.33 96.0 -0.229 93.0 noncon
hsa-miR-3161 607-614 -0.329 98.0 -0.229 97.0 noncon
hsa-miR-1294 4639-4645 -0.329 97.0 0.0 18.0 noncon
hsa-miR-3675-3p 1116-1123 -0.327 99.0 -0.227 98.0 noncon
hsa-miR-98-3p 1374-1381 -0.327 99.0 -0.227 98.0 noncon
hsa-miR-136-5p 5317-5324 -0.324 97.0 0.0 30.0 noncon
hsa-miR-30b-3p 368-375 -0.322 96.0 -0.224 93.0 noncon
hsa-miR-376a-2-5p 471-478 -0.322 98.0 -0.224 96.0 noncon
hsa-miR-186-5p 5588-5595 -0.319 99.0 0.0 59.0 noncon
hsa-miR-6836-5p 962-968 -0.317 95.0 -0.221 92.0 noncon
hsa-miR-6761-5p 1258-1264 -0.315 98.0 -0.219 97.0 noncon
hsa-miR-4274 70-76 -0.313 97.0 -0.218 95.0 noncon
hsa-miR-3925-5p 5505-5512 -0.311 99.0 0.0 52.0 noncon
hsa-let-7b-3p 1374-1381 -0.311 99.0 -0.217 98.0 noncon
hsa-let-7a-3p 1374-1381 -0.311 99.0 -0.217 98.0 noncon
hsa-miR-4659b-5p 5028-5034 -0.311 97.0 0.0 27.0 noncon
hsa-miR-2115-5p 5374-5381 -0.309 96.0 0.0 11.0 noncon
hsa-miR-4491 502-508 -0.308 97.0 -0.215 94.0 noncon
hsa-miR-4520-2-3p 1855-1862 -0.308 93.0 0.0 9.0 noncon
hsa-miR-6762-5p 2294-2301 -0.306 91.0 0.0 6.0 noncon
hsa-miR-6845-5p 2294-2301 -0.306 91.0 0.0 7.0 noncon
hsa-miR-552-3p 3363-3370 -0.305 96.0 0.0 20.0 noncon
hsa-miR-218-2-3p 3277-3284 -0.305 97.0 0.0 32.0 noncon
hsa-miR-6508-3p 2320-2327 -0.304 96.0 0.0 16.0 noncon
hsa-miR-892b 1356-1362 -0.304 95.0 -0.212 92.0 noncon
hsa-miR-4794 1355-1361 -0.304 98.0 -0.212 97.0 noncon
hsa-miR-4768-5p 483-490 -0.3 96.0 -0.209 95.0 noncon
hsa-miR-625-5p 4408-4415 -0.299 90.0 0.0 6.0 noncon
hsa-miR-1247-3p 202-208 -0.299 93.0 -0.209 86.0 noncon
hsa-miR-219a-1-3p 2143-2150 -0.298 95.0 0.0 11.0 noncon
hsa-miR-203b-3p 658-665 -0.298 98.0 -0.208 96.0 noncon
hsa-miR-4659a-5p 5028-5034 -0.297 96.0 0.0 29.0 noncon
hsa-miR-605-3p 1324-1330 -0.296 95.0 -0.207 90.0 noncon
hsa-miR-6754-3p 4895-4901 -0.293 96.0 0.0 30.0 noncon
hsa-miR-383-5p.1 17-23 -0.293 94.0 -0.293 96.0 noncon
hsa-miR-214-3p 442-448 -0.292 98.0 -0.204 95.0 noncon
hsa-miR-4316 3582-3588 -0.29 96.0 0.0 32.0 noncon
hsa-miR-3187-3p 668-674 -0.289 98.0 -0.202 97.0 noncon
hsa-miR-29c-3p 567-573 -0.288 91.0 -0.201 88.0 noncon
hsa-miR-29a-3p 567-573 -0.288 91.0 -0.201 88.0 noncon
hsa-miR-6794-3p 1993-2000 -0.288 96.0 0.0 24.0 noncon
hsa-miR-5096 465-472 -0.288 99.0 -0.201 98.0 noncon
hsa-miR-29b-3p 567-573 -0.288 91.0 -0.201 88.0 noncon
hsa-miR-6868-3p 5039-5046 -0.286 96.0 0.0 24.0 noncon
hsa-miR-605-5p 534-540 -0.283 98.0 -0.198 96.0 noncon
hsa-miR-664a-5p 1355-1361 -0.283 98.0 -0.198 95.0 noncon
hsa-miR-1238-3p 39-45 -0.281 99.0 -0.281 99.0 noncon
hsa-miR-340-5p 5591-5598 -0.281 99.0 0.0 52.0 noncon
hsa-miR-320e 1415-1421 -0.28 97.0 -0.196 94.0 noncon
hsa-miR-3619-5p 442-448 -0.28 97.0 -0.196 95.0 noncon
hsa-miR-1179 602-608 -0.278 97.0 -0.194 96.0 noncon
hsa-miR-3613-3p 2250-2257 -0.278 99.0 0.0 25.0 noncon
hsa-miR-5007-3p 1584-1591 -0.278 98.0 0.0 27.0 noncon
hsa-miR-4786-3p 5530-5536 -0.278 95.0 0.0 11.0 noncon
hsa-miR-3117-5p 1032-1038 -0.277 96.0 -0.194 92.0 noncon
hsa-miR-3198 4960-4967 -0.274 97.0 0.0 51.0 noncon
hsa-miR-485-3p 5458-5465 -0.273 97.0 0.0 18.0 noncon
hsa-miR-412-3p 4895-4901 -0.272 96.0 0.0 28.0 noncon
hsa-miR-5006-5p 28-34 -0.271 94.0 -0.271 96.0 noncon
hsa-miR-939-3p 2164-2171 -0.27 95.0 0.0 36.0 noncon
hsa-miR-6873-3p 2904-2911 -0.27 95.0 0.0 21.0 noncon
hsa-miR-4292 2102-2108 -0.269 94.0 0.0 21.0 noncon
hsa-miR-4798-3p 1011-1017 -0.268 97.0 -0.188 95.0 noncon
hsa-miR-7703 162-169 -0.267 97.0 -0.187 95.0 noncon
hsa-miR-6080 4331-4337 -0.267 93.0 0.0 21.0 noncon
hsa-miR-6507-3p 1313-1319 -0.267 97.0 -0.187 95.0 noncon
hsa-miR-655-5p 4638-4644 -0.267 95.0 0.0 14.0 noncon
hsa-miR-7847-3p 194-200 -0.266 92.0 -0.186 86.0 noncon
hsa-miR-6833-3p 483-490 -0.266 96.0 -0.186 94.0 noncon
hsa-miR-200b-5p 1144-1150 -0.262 97.0 -0.184 96.0 noncon
hsa-miR-200a-5p 1144-1150 -0.262 97.0 -0.184 96.0 noncon
hsa-miR-1273e 210-217 -0.26 95.0 -0.182 92.0 noncon
hsa-miR-125a-5p 5237-5243 -0.259 93.0 0.0 20.0 noncon
hsa-miR-3927-5p 2405-2412 -0.259 95.0 0.0 11.0 noncon
hsa-miR-7150 1918-1924 -0.258 94.0 0.0 23.0 noncon
hsa-miR-198 1310-1316 -0.258 96.0 -0.181 93.0 noncon
hsa-miR-921 3584-3590 -0.258 93.0 0.0 15.0 noncon
hsa-miR-8076 2017-2024 -0.257 95.0 0.0 11.0 noncon
hsa-miR-6849-3p 156-163 -0.255 96.0 -0.179 95.0 noncon
hsa-miR-6505-3p 3922-3929 -0.255 93.0 0.0 15.0 noncon
hsa-miR-34a-3p 3391-3397 -0.255 96.0 0.0 41.0 noncon
hsa-miR-3973 1370-1376 -0.254 98.0 -0.178 96.0 noncon
hsa-miR-4791 737-743 -0.254 93.0 -0.178 90.0 noncon
hsa-miR-3972 28-34 -0.254 94.0 -0.254 96.0 noncon
hsa-miR-7107-5p 2151-2157 -0.253 91.0 0.0 7.0 noncon
hsa-miR-3613-3p 2913-2919 -0.252 99.0 0.0 25.0 noncon
hsa-miR-4653-3p 3770-3776 -0.251 96.0 0.0 12.0 noncon
hsa-miR-4504 2866-2873 -0.251 95.0 0.0 15.0 noncon
hsa-miR-7976 5238-5244 -0.251 95.0 0.0 13.0 noncon
hsa-miR-3190-3p 5122-5129 -0.25 94.0 0.0 18.0 noncon