GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
STT3B



General Information
Gene Symbol:   
STT3B
Protein name:   
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT66
E.C. No:   
2.4.99.18
Organs:   
Reaction (SNFG):   
→
Reaction (IUBMB):   
No specified reaction.
About

• A multi-membrane protein ER membrane associated protein complex that mediates the en-bloc transfer of 14 sugar LLO to N-X-S/T motif in nascent polypeptide
• STT3 is the catalytic core of this complex
• Stt3 forms a complex with the accessory membrane proteins DDOST/OST48, RPN1/ribophorin I, RPN2/ribophorin II, DAD1, Ost4, TUSC3/N33 and MGAT1/IAP in mammals for maximal activity.
• There are two STT3 genes: STT3A and STT3B. Human Stt3A and Stt3B have redundant functions in the co-translational N-glycosylation. Stt3B also mediates the post-translational N-glycosylation after some time, rather than just at the time when the protein enters the ER from the ribosome.
• Glc[0-3]Man[0-9]GlcNAc2β1-PP-Dol + Asn-Xaa-Ser/Thr -> Dol-PP + Glc[0-3]Man[0-9]GlcNAc2β1-Asn-Xaa-Ser/Thr.

Related Pathway
KEGG ORTHOLOGY:   
K07151
KEGG Pathway:   
ko00510  ko00513  ko01100  ko04141 
Reactome:   
No Record
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q8TCJ2 
NCBI GeneID:   
201595
GlyMap GeneID:   
201595
DNA aRefSeq:   
NM_178862.2 
Protein RefSeq:   
NP_849193.1 
BRENDA:   
Kinetic info.
OMIM number:   
608605
GeneCards database:   
STT3B
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     ER
Substrate:  
     Glc(a1-2)Glc(a1-3)Man(a1-2)Man(a1-2)Man(a1-3)[Man(a1-2)Man(a1-6)[Man(a1-2)Man(a1-3)]Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc(a1-?)Dol-PP
Product:  
     Glc(a1-2)Glc(a1-3)Man(a1-2)Man(a1-2)Man(a1-3)[Man(a1-2)Man(a1-6)[Man(a1-2)Man(a1-3)]Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc(b1-?)Asn
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 50 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 140 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-671-3p 433-439 -0.475 95.0 -0.391 93.0 noncon
hsa-miR-129-2-3p 715-722 -0.467 99.0 -0.384 99.0 con
hsa-miR-129-1-3p 715-722 -0.467 99.0 -0.384 99.0 con
hsa-miR-4671-3p 1069-1076 -0.453 98.0 -0.373 97.0 noncon
hsa-miR-6500-3p 403-410 -0.435 99.0 -0.359 99.0 noncon
hsa-miR-4666b 817-824 -0.423 98.0 -0.349 98.0 noncon
hsa-miR-671-3p 44-50 -0.418 92.0 -0.418 95.0 noncon
hsa-miR-4330 270-277 -0.403 99.0 -0.334 99.0 noncon
hsa-miR-6081 969-976 -0.391 97.0 -0.323 96.0 noncon
hsa-miR-561-5p 488-495 -0.386 98.0 -0.32 98.0 noncon
hsa-miR-3157-5p 1406-1413 -0.381 98.0 -0.315 98.0 noncon
hsa-miR-20a-3p 1502-1509 -0.367 98.0 -0.223 95.0 noncon
hsa-miR-3650 1225-1232 -0.346 97.0 -0.287 97.0 noncon
hsa-miR-490-3p 110-117 -0.344 98.0 -0.344 99.0 noncon
hsa-miR-4645-5p 914-921 -0.339 98.0 -0.281 98.0 noncon
hsa-miR-505-3p.1 638-645 -0.327 94.0 -0.271 93.0 noncon
hsa-miR-4673 914-921 -0.305 98.0 -0.253 98.0 noncon
hsa-miR-152-5p 94-101 -0.305 95.0 -0.305 96.0 noncon
hsa-miR-320e 1108-1115 -0.304 97.0 -0.253 97.0 noncon
hsa-miR-431-5p 201-208 -0.303 96.0 -0.252 95.0 con
hsa-miR-7848-3p 87-93 -0.302 94.0 -0.302 96.0 noncon
hsa-miR-4457 36-43 -0.298 97.0 -0.298 98.0 noncon
hsa-miR-6501-3p 568-575 -0.298 98.0 -0.248 97.0 noncon
hsa-miR-4295 1069-1075 -0.294 96.0 -0.244 96.0 con
hsa-miR-301b-3p 1069-1075 -0.294 96.0 -0.244 95.0 con
hsa-miR-301a-3p 1069-1075 -0.294 96.0 -0.244 95.0 con
hsa-miR-3171 700-707 -0.284 94.0 -0.236 94.0 noncon
hsa-miR-130b-3p 1069-1075 -0.282 95.0 -0.235 95.0 con
hsa-miR-6830-5p 487-494 -0.281 97.0 -0.234 97.0 noncon
hsa-miR-610 60-67 -0.273 95.0 -0.273 96.0 noncon
hsa-miR-130a-3p 1069-1075 -0.27 95.0 -0.225 94.0 con
hsa-miR-3666 1069-1075 -0.27 95.0 -0.225 94.0 con
hsa-miR-455-5p 320-326 -0.269 93.0 -0.225 94.0 con
hsa-miR-493-5p 118-125 -0.266 97.0 -0.266 98.0 con
hsa-miR-4659a-5p 743-750 -0.265 94.0 -0.221 94.0 noncon
hsa-miR-3914 1383-1389 -0.264 95.0 -0.22 95.0 noncon
hsa-miR-6806-3p 424-430 -0.263 95.0 -0.22 95.0 noncon
hsa-miR-549a 1056-1063 -0.263 93.0 -0.219 93.0 noncon
hsa-miR-136-5p 1083-1090 -0.262 96.0 -0.218 95.0 con
hsa-miR-4291 1314-1320 -0.262 95.0 -0.218 95.0 noncon
hsa-miR-6892-5p 987-994 -0.252 94.0 -0.21 94.0 noncon
hsa-miR-4317 134-140 -0.25 93.0 -0.25 95.0 noncon
hsa-miR-4780 713-719 -0.25 93.0 -0.208 92.0 noncon