GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ALG8



General Information
Gene Symbol:   
ALG8
Protein name:   
Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT57
E.C. No:   
2.4.1.265
Organs:   
Reaction (SNFG):   
+
→
+ P-Dol
Reaction (IUBMB):   
No specified reaction.
About

• Dol-P-Glc is formed from UDP-Glc using ALG5
• Three Glc residues are then transferred from Dol-P-Glc to the LLO using ALG6 [α(1,3)GlcT], ALG8 [α(1,3)GlcT] and ALG10 [α(1,2)GlcT].

Related Pathway
KEGG ORTHOLOGY:   
K03849
KEGG Pathway:   
ko00510  ko01100 
Reactome:   
R-HSA-446193
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9BVK2 
NCBI GeneID:   
79053
GlyMap GeneID:   
79053
DNA aRefSeq:   
NM_001007027.2 
Protein RefSeq:   
NP_001007028.1 
BRENDA:   
Kinetic info.
OMIM number:   
608103
GeneCards database:   
ALG8
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     ER
Substrate:  
     Glc(a1-3)Man(a1-2)Man(a1-2)Man(a1-3)
Product:  
     Glc(a1-3)Glc(a1-3)Man(a1-2)Man(a1-2)Man(a1-3)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 200 180 160 140 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-431-5p 233-240 -0.582 99.0 -0.582 99.0 con
hsa-miR-24-1-5p 171-178 -0.561 98.0 -0.561 99.0 noncon
hsa-miR-24-2-5p 171-178 -0.553 98.0 -0.553 98.0 noncon
hsa-miR-3907 362-369 -0.524 99.0 0.0 18.0 noncon
hsa-miR-192-3p 229-236 -0.523 99.0 -0.523 99.0 noncon
hsa-miR-3179 132-139 -0.517 99.0 -0.517 99.0 noncon
hsa-miR-5681a 105-112 -0.47 99.0 -0.47 99.0 noncon
hsa-miR-4314 15-22 -0.459 99.0 -0.459 99.0 noncon
hsa-miR-655-5p 152-159 -0.454 99.0 -0.454 99.0 noncon
hsa-miR-665 114-121 -0.444 99.0 -0.444 99.0 con
hsa-miR-584-3p 258-264 -0.417 98.0 -0.417 99.0 noncon
hsa-miR-6844 238-245 -0.395 99.0 -0.395 99.0 noncon
hsa-miR-302f 280-287 -0.385 98.0 0.0 24.0 noncon
hsa-miR-135a-3p 476-482 -0.377 97.0 0.0 7.0 noncon
hsa-miR-3190-3p 372-379 -0.37 98.0 0.0 20.0 noncon
hsa-miR-6757-5p 138-144 -0.361 98.0 -0.361 98.0 noncon
hsa-miR-5584-5p 474-481 -0.342 99.0 0.0 22.0 noncon
hsa-miR-1271-5p 274-280 -0.333 96.0 0.0 33.0 noncon
hsa-miR-7162-3p 154-160 -0.331 98.0 -0.331 99.0 noncon
hsa-miR-5187-5p 137-143 -0.327 96.0 -0.327 97.0 noncon
hsa-miR-494-3p 203-210 -0.327 99.0 -0.327 99.0 noncon
hsa-miR-664a-3p 248-255 -0.326 99.0 -0.326 99.0 noncon
hsa-miR-4802-5p 435-442 -0.323 97.0 0.0 24.0 noncon
hsa-miR-6851-5p 111-117 -0.318 95.0 -0.318 96.0 noncon
hsa-miR-1229-3p 418-425 -0.311 96.0 0.0 33.0 noncon
hsa-miR-5008-5p 73-79 -0.311 95.0 -0.311 96.0 noncon
hsa-miR-6511a-3p 417-424 -0.302 96.0 0.0 13.0 noncon
hsa-miR-6511b-3p 417-424 -0.302 96.0 0.0 13.0 noncon
hsa-miR-10b-3p 534-541 -0.3 98.0 0.0 34.0 noncon
hsa-miR-4320 600-606 -0.297 96.0 0.0 21.0 noncon
hsa-miR-525-5p 77-84 -0.297 97.0 -0.297 97.0 noncon
hsa-miR-4771 84-90 -0.294 95.0 -0.294 96.0 noncon
hsa-miR-520a-5p 77-84 -0.289 96.0 -0.289 97.0 noncon
hsa-miR-6792-5p 262-268 -0.287 98.0 -0.287 98.0 noncon
hsa-miR-3942-3p 456-463 -0.281 98.0 0.0 33.0 noncon
hsa-miR-96-5p 274-280 -0.281 94.0 0.0 30.0 noncon
hsa-miR-6864-5p 133-139 -0.28 96.0 -0.28 97.0 noncon
hsa-miR-583 31-37 -0.275 98.0 -0.275 99.0 noncon
hsa-miR-3911 374-380 -0.275 95.0 0.0 11.0 noncon
hsa-miR-874-5p 71-77 -0.268 90.0 -0.268 93.0 noncon
hsa-miR-617 81-87 -0.267 93.0 -0.267 95.0 noncon
hsa-miR-196a-3p 320-326 -0.266 93.0 0.0 8.0 noncon
hsa-miR-3927-3p 56-62 -0.266 94.0 -0.266 96.0 noncon
hsa-miR-3936 131-137 -0.26 95.0 -0.26 96.0 noncon
hsa-miR-6739-5p 473-479 -0.258 96.0 0.0 27.0 noncon
hsa-miR-6831-5p 56-62 -0.258 93.0 -0.258 95.0 noncon
hsa-miR-4276 445-451 -0.258 95.0 0.0 17.0 noncon