SubPathClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
FUT7



General Information
Gene Symbol:   
FUT7
Protein name:   
Alpha-(1,3)-fucosyltransferase 7
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT10
E.C. No:   
2.4.1.152
Organs:   
Reaction (SNFG):   
+
→
+ GDP
Reaction (IUBMB):   
No specified reaction.
About

• FUT7 (and FUT4) is ubiquitously expressed in all populations of peripheral blood cells including granulocytes, monocytes, and lymphocytes. It is also expressed in High Endothelial Venules (HEV).
• In blood cells, this is the dominant enzyme forming the sialyl Lewis-X structure and also 6 sulfo-sialyl Lewis-X. It exhibits strict enzyme specificity for sialyl lactosamine structures.
• It is tightly transcriptionally regulated, and plays a critical role in controlling L-, E- and P-selectin dependent leukocyte-endothelial cell adhesion interactions across species.
• Other FUTs like FUT3, 4, 5, 6, particularly FUT3 and 6, may also form the sLeX glycan in other tissue types.

Related Pathway
KEGG ORTHOLOGY:   
K07635
KEGG Pathway:   
ko00601  ko01100 
Reactome:   
R-HSA-9037629
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q11130 
NCBI GeneID:   
2529
GlyMap GeneID:   
2529
DNA aRefSeq:   
NM_004479.3 
Protein RefSeq:   
NP_004470.1 
BRENDA:   
Kinetic info.
OMIM number:   
602030
GeneCards database:   
FUT7
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     NeuAc(a2-3)Gal(b1-4)GlcNAc(b1-?)
Product:  
     NeuAc(a2-3)Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.75e-06    (pmol/cell • hr)       KM:    1.40e+09    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000314412 GATA2 STAT5A ZNF263 ZNF121 ZBTB33 RELB PPARG NFE2 STAT5A USF2 BHLHE40 SP2 TAL1 CTCF ERG NFE2 NFKB1 RELB NFKB2 TAL1 SP2 RUNX1 BHLHE40 STAT5A PPARG FLI1 IKZF1 TFAP4 TWIST1 TCF12 EBF1 GABPA SPIB CTCF CTCF EBF3 LYL1 TCF3 TCF4 STAT5A ELF1 CTCF TCF3 EBF1 EGR3 SP2 SP1 SP5 NR2F6 HMG20A GATA6 HMG20B GABPA KLF1 SKI NR2F2
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-1306-5p 164-171 -0.5 99.0 -0.5 99.0 noncon
hsa-miR-876-3p 55-62 -0.477 98.0 -0.477 98.0 noncon
hsa-miR-6865-3p 281-288 -0.465 99.0 -0.465 99.0 noncon
hsa-miR-6720-5p 273-280 -0.412 98.0 -0.412 99.0 noncon
hsa-miR-6887-3p 211-218 -0.404 96.0 -0.404 97.0 noncon
hsa-miR-6512-3p 273-280 -0.404 98.0 -0.404 99.0 noncon
hsa-miR-296-5p 453-460 -0.402 95.0 -0.402 96.0 noncon
hsa-miR-4763-3p 407-414 -0.387 98.0 -0.387 98.0 noncon
hsa-miR-4290 504-510 -0.385 95.0 -0.385 96.0 noncon
hsa-miR-6826-3p 212-219 -0.361 96.0 -0.361 97.0 noncon
hsa-miR-1207-5p 407-414 -0.337 97.0 -0.337 97.0 noncon
hsa-miR-6810-3p 124-130 -0.329 95.0 -0.329 97.0 noncon
hsa-miR-7160-3p 295-301 -0.329 96.0 -0.329 97.0 noncon
hsa-miR-1825 147-154 -0.32 97.0 -0.32 98.0 noncon
hsa-miR-6801-3p 124-130 -0.319 95.0 -0.319 96.0 noncon
hsa-miR-6889-3p 107-114 -0.317 96.0 -0.317 97.0 noncon
hsa-miR-7108-3p 449-455 -0.313 90.0 -0.313 92.0 noncon
hsa-miR-125b-5p 495-502 -0.31 96.0 -0.31 97.0 noncon
hsa-miR-378a-5p 116-123 -0.309 98.0 -0.309 99.0 noncon
hsa-miR-4726-3p 344-351 -0.309 98.0 -0.309 98.0 noncon
hsa-miR-6821-3p 112-119 -0.306 97.0 -0.306 98.0 noncon
hsa-miR-7162-5p 119-126 -0.295 96.0 -0.295 97.0 noncon
hsa-miR-6861-3p 428-434 -0.295 92.0 -0.295 95.0 noncon
hsa-miR-4664-5p 314-321 -0.295 94.0 -0.295 96.0 noncon
hsa-miR-4319 495-502 -0.294 95.0 -0.294 96.0 noncon
hsa-miR-125a-5p 495-502 -0.294 95.0 -0.294 96.0 noncon
hsa-miR-5701 515-522 -0.293 97.0 -0.293 98.0 noncon
hsa-miR-874-5p 336-342 -0.293 92.0 -0.293 94.0 noncon
hsa-miR-6811-3p 394-401 -0.29 96.0 -0.29 97.0 noncon
hsa-miR-5193 162-168 -0.284 93.0 -0.284 95.0 noncon
hsa-miR-4456 57-63 -0.284 95.0 -0.284 96.0 noncon
hsa-miR-4523 363-369 -0.282 92.0 -0.282 94.0 noncon
hsa-miR-342-5p 314-321 -0.281 93.0 -0.281 95.0 noncon
hsa-miR-516b-3p 119-126 -0.279 95.0 -0.279 97.0 noncon
hsa-miR-516a-3p 119-126 -0.279 95.0 -0.279 97.0 noncon
hsa-miR-6511b-3p 167-173 -0.273 94.0 -0.273 96.0 noncon
hsa-miR-6511a-3p 167-173 -0.273 94.0 -0.273 96.0 noncon
hsa-miR-3918 375-381 -0.27 94.0 -0.27 95.0 noncon
hsa-miR-3649 429-435 -0.264 92.0 -0.264 94.0 noncon
hsa-miR-3173-5p 292-298 -0.264 94.0 -0.264 96.0 noncon
hsa-miR-2115-5p 120-126 -0.264 93.0 -0.264 95.0 noncon
hsa-miR-1254 60-66 -0.258 96.0 -0.258 97.0 noncon
hsa-miR-1229-3p 167-173 -0.257 94.0 -0.257 96.0 noncon
hsa-miR-939-3p 456-463 -0.25 94.0 -0.25 96.0 noncon