SubPathClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ST3GAL3



General Information
Gene Symbol:   
ST3GAL3
Protein name:   
CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT29
E.C. No:   
2.4.99.6
Organs:   
Reaction (SNFG):   
+
→
+ CMP
+
→
+ CMP
+
→
+ CMP
Reaction (IUBMB):   
No specified reaction.
About

• ST3Gal-III prefers Galβ1-3GlcNAc, but also acts on Galβ1-4GlcNAc (Km < 0.1 mM) and Galβ1-3GalNAc (Km=4 mM) termini on glycoproteins, oligosaccharides and glycolipids.
• Evidence suggests that the enzyme may prefer the gangliotetraose terminus (Galβ1-3GalNAcβ1-4Gal) compared to core 1 O-linked glycans (Galβ1-3GalNAcα1-Ser/Thr).
• Mutations in the ST3GAL3 gene in humans are associated with rare severe familial intellectual disability
• ST3GAL3 gene is widely expressed in human tissues, with higher mRNA levels in the brain, skeletal muscle, adrenal gland, uterus, and lymphocytes.
• Likely to play a role in regulating Siglec function.

Related Pathway
KEGG ORTHOLOGY:   
K00781
KEGG Pathway:   
ko00513  ko00514  ko00515  ko00533  ko00601  ko01100 
Reactome:   
R-HSA-1912420
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q11203 
NCBI GeneID:   
6487
GlyMap GeneID:   
6487
DNA aRefSeq:   
NM_001270459.1 
Protein RefSeq:   
NP_001257388.1 
BRENDA:   
Kinetic info.
OMIM number:   
606494
GeneCards database:   
ST3GAL3
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), O-linked (other), GSL
Compartment:  
     
Substrate:  
     Gal(b1-4)GlcNAc(b1-?) or Gal(b1-3)GlcNAc(b1-?) or Gal(b1-3)GalNAc(b1-?)
Product:  
     NeuAc(a2-3)Gal(b1-4)GlcNAc(b1-?) or NeuAc(a2-3)Gal(b1-3)GlcNAc(b1-?) or NeuAc(a2-3)Gal(b1-3)GalNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    7.26e-05    (pmol/cell • hr)       KM:    2.60e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-3196 981-988 -0.681 98.0 -0.681 98.0 noncon
hsa-miR-3180-3p 981-988 -0.652 98.0 -0.652 98.0 noncon
hsa-miR-3180 981-988 -0.652 98.0 -0.652 98.0 noncon
hsa-miR-6816-5p 981-988 -0.652 98.0 -0.652 98.0 noncon
hsa-miR-210-3p 242-249 -0.613 98.0 -0.613 99.0 con
hsa-miR-103b 164-171 -0.586 99.0 -0.586 99.0 noncon
hsa-miR-6804-5p 43-50 -0.582 99.0 -0.582 99.0 noncon
hsa-miR-1909-3p 191-198 -0.568 99.0 -0.568 99.0 noncon
hsa-miR-3162-3p 675-682 -0.567 99.0 -0.567 99.0 noncon
hsa-miR-6840-5p 82-89 -0.562 98.0 -0.562 98.0 noncon
hsa-miR-6722-3p 191-198 -0.555 99.0 -0.555 99.0 noncon
hsa-miR-92b-5p 1220-1227 -0.552 97.0 -0.552 98.0 noncon
hsa-miR-4455 178-185 -0.515 98.0 -0.515 98.0 noncon
hsa-miR-5580-5p 374-381 -0.48 98.0 -0.48 99.0 noncon
hsa-miR-7110-5p 1231-1238 -0.468 95.0 -0.468 96.0 noncon
hsa-miR-6890-3p 112-119 -0.452 99.0 -0.452 99.0 noncon
hsa-miR-6842-5p 1231-1238 -0.452 95.0 -0.452 96.0 noncon
hsa-miR-2115-5p 1239-1246 -0.45 98.0 -0.45 99.0 noncon
hsa-miR-3189-3p 1192-1199 -0.442 98.0 -0.442 99.0 noncon
hsa-miR-3651 989-996 -0.441 97.0 -0.441 98.0 noncon
hsa-miR-137 1243-1250 -0.44 97.0 -0.44 98.0 con
hsa-miR-3688-5p 1111-1118 -0.438 99.0 -0.438 99.0 noncon
hsa-miR-4745-5p 916-923 -0.422 98.0 -0.422 98.0 noncon
hsa-miR-133a-3p.2 1173-1180 -0.402 96.0 -0.402 97.0 noncon
hsa-miR-133b 1173-1180 -0.402 96.0 -0.402 97.0 noncon
hsa-miR-3115 15-21 -0.401 96.0 -0.401 98.0 noncon
hsa-miR-4487 868-875 -0.399 98.0 -0.399 98.0 noncon
hsa-miR-383-5p.2 303-310 -0.395 99.0 -0.395 99.0 noncon
hsa-miR-6752-5p 1231-1238 -0.392 94.0 -0.392 95.0 noncon
hsa-miR-342-5p 188-194 -0.39 97.0 -0.39 98.0 noncon
hsa-miR-6847-3p 172-179 -0.386 99.0 -0.386 99.0 noncon
hsa-miR-6884-5p 504-511 -0.38 98.0 -0.38 99.0 noncon
hsa-miR-6864-5p 59-66 -0.379 98.0 -0.379 99.0 noncon
hsa-miR-555 1201-1208 -0.376 96.0 -0.376 98.0 noncon
hsa-miR-4323 826-833 -0.375 98.0 -0.375 99.0 noncon
hsa-miR-485-5p 504-511 -0.372 98.0 -0.372 98.0 noncon
hsa-miR-7855-5p 1102-1109 -0.37 98.0 -0.37 98.0 noncon
hsa-miR-4531 621-628 -0.366 98.0 -0.366 99.0 noncon
hsa-miR-1226-3p 280-287 -0.362 98.0 -0.362 98.0 noncon
hsa-miR-4446-3p 925-932 -0.361 97.0 -0.361 98.0 noncon
hsa-miR-3162-3p 663-669 -0.353 94.0 -0.353 96.0 noncon
hsa-miR-6762-3p 371-378 -0.352 97.0 -0.352 97.0 noncon
hsa-miR-326 405-412 -0.35 98.0 -0.35 98.0 con
hsa-miR-1180-3p 610-616 -0.348 90.0 -0.348 93.0 noncon
hsa-miR-4664-5p 188-194 -0.345 96.0 -0.345 97.0 noncon
hsa-miR-7847-3p 1058-1065 -0.343 96.0 -0.343 97.0 noncon
hsa-miR-6757-5p 91-98 -0.342 97.0 -0.342 98.0 noncon
hsa-miR-6749-5p 1093-1099 -0.342 92.0 -0.342 94.0 noncon
hsa-miR-6805-5p 861-867 -0.322 91.0 -0.322 93.0 noncon
hsa-miR-6779-3p 987-994 -0.315 97.0 -0.315 98.0 noncon
hsa-miR-4481 916-923 -0.314 96.0 -0.314 97.0 noncon
hsa-miR-330-5p 405-412 -0.313 97.0 -0.313 98.0 con
hsa-miR-6762-3p 478-485 -0.306 95.0 -0.306 96.0 noncon
hsa-miR-5703 620-627 -0.306 97.0 -0.306 98.0 noncon
hsa-miR-6131 494-501 -0.305 98.0 -0.305 98.0 noncon
hsa-miR-4758-3p 788-795 -0.302 95.0 -0.302 97.0 noncon
hsa-miR-489-3p 708-715 -0.301 98.0 -0.301 99.0 noncon
hsa-miR-6865-3p 1020-1027 -0.301 96.0 -0.301 97.0 noncon
hsa-miR-4701-3p 180-186 -0.299 96.0 -0.299 97.0 noncon
hsa-miR-4434 620-627 -0.298 97.0 -0.298 98.0 noncon
hsa-miR-4433a-5p 584-591 -0.296 95.0 -0.296 97.0 noncon
hsa-miR-4252 313-320 -0.296 98.0 -0.296 98.0 noncon
hsa-miR-661 352-359 -0.295 96.0 -0.295 97.0 noncon
hsa-miR-6773-5p 328-334 -0.295 94.0 -0.295 95.0 noncon
hsa-miR-6724-5p 754-760 -0.294 94.0 -0.294 96.0 noncon
hsa-miR-4663 474-481 -0.292 97.0 -0.292 97.0 noncon
hsa-miR-4758-3p 825-831 -0.288 95.0 -0.288 96.0 noncon
hsa-miR-1262 180-186 -0.287 96.0 -0.287 97.0 noncon
hsa-miR-6773-5p 754-760 -0.286 93.0 -0.286 95.0 noncon
hsa-miR-371a-3p 124-130 -0.286 96.0 -0.286 97.0 noncon
hsa-miR-6736-5p 180-186 -0.283 96.0 -0.283 97.0 noncon
hsa-miR-1273g-3p 312-318 -0.282 97.0 -0.282 98.0 noncon
hsa-miR-6724-5p 328-334 -0.281 94.0 -0.281 95.0 noncon
hsa-miR-7848-3p 672-678 -0.275 92.0 -0.275 95.0 noncon
hsa-miR-3191-5p 406-413 -0.275 97.0 -0.275 97.0 noncon
hsa-miR-15a-3p 69-75 -0.267 97.0 -0.267 98.0 noncon
hsa-miR-1343-5p 1053-1059 -0.265 94.0 -0.265 95.0 noncon
hsa-miR-484 1262-1268 -0.265 97.0 0.0 0.0 noncon
hsa-miR-3155a 1262-1268 -0.262 97.0 0.0 0.0 noncon
hsa-miR-6762-3p 455-462 -0.261 93.0 -0.261 95.0 noncon
hsa-miR-5002-3p 148-154 -0.261 91.0 -0.261 94.0 noncon
hsa-miR-6733-3p 877-883 -0.259 95.0 -0.259 97.0 noncon
hsa-miR-4662a-5p 162-168 -0.258 95.0 -0.258 97.0 noncon
hsa-miR-2861 565-572 -0.257 93.0 -0.257 94.0 noncon
hsa-miR-6721-5p 55-61 -0.256 91.0 -0.256 93.0 noncon
hsa-miR-3155b 1262-1268 -0.251 97.0 0.0 0.0 noncon
hsa-miR-4329 486-493 -0.25 97.0 -0.25 98.0 noncon