GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
FUT7



General Information
Gene Symbol:   
FUT7
Protein name:   
Alpha-(1,3)-fucosyltransferase 7
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT10
E.C. No:   
2.4.1.152
Organs:   
Reaction (SNFG):   
+
→
+ GDP
Reaction (IUBMB):   
No specified reaction.
About

• FUT7 (and FUT4) is ubiquitously expressed in all populations of peripheral blood cells including granulocytes, monocytes, and lymphocytes. It is also expressed in High Endothelial Venules (HEV).
• In blood cells, this is the dominant enzyme forming the sialyl Lewis-X structure and also 6 sulfo-sialyl Lewis-X. It exhibits strict enzyme specificity for sialyl lactosamine structures.
• It is tightly transcriptionally regulated, and plays a critical role in controlling L-, E- and P-selectin dependent leukocyte-endothelial cell adhesion interactions across species.
• Other FUTs like FUT3, 4, 5, 6, particularly FUT3 and 6, may also form the sLeX glycan in other tissue types.

Related Pathway
KEGG ORTHOLOGY:   
K07635
KEGG Pathway:   
ko00601  ko01100 
Reactome:   
R-HSA-9037629
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q11130 
NCBI GeneID:   
2529
GlyMap GeneID:   
2529
DNA aRefSeq:   
NM_004479.3 
Protein RefSeq:   
NP_004470.1 
BRENDA:   
Kinetic info.
OMIM number:   
602030
GeneCards database:   
FUT7
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     NeuAc(a2-3)Gal(b1-4)GlcNAc(b1-?)
Product:  
     NeuAc(a2-3)Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.75e-06    (pmol/cell • hr)       KM:    1.40e+09    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000314412 GATA2 STAT5A ZNF263 ZNF121 ZBTB33 RELB PPARG NFE2 STAT5A USF2 BHLHE40 SP2 TAL1 CTCF ERG NFE2 NFKB1 RELB NFKB2 TAL1 SP2 RUNX1 BHLHE40 STAT5A PPARG FLI1 IKZF1 TFAP4 TWIST1 TCF12 EBF1 GABPA SPIB CTCF CTCF EBF3 LYL1 TCF3 TCF4 STAT5A ELF1 CTCF TCF3 EBF1 EGR3 SP2 SP1 SP5 NR2F6 HMG20A GATA6 HMG20B GABPA KLF1 SKI NR2F2
miRNA-GlycoEnzyme Interactome {ref. 9}
FUT7/hsa-miR-6821-3p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...GGAGGCUGGGCAC AGAGGUCA ...
3'   GACACGCCUCGCC UCUCCAG U
 8mer 112-119 -0.306 97.0 -0.306 98.0 noncon