SubPathClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ST3GAL4



General Information
Gene Symbol:   
ST3GAL4
Protein name:   
CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 4
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT29
E.C. No:   
2.4.99.2
Organs:   
Reaction (SNFG):   
+
→
+ CMP
+
→
+ CMP
Reaction (IUBMB):   
No specified reaction.
About

• ST3Gal-4 transfers α2,3sialic acid preferentially to Galβ1-4GlcNAc (Type-II, Km=0.22mM), but may also act on Type-I (Galβ1-3GlcNAc, Km=0.75mM) and Type-III (Galβ1-3GalNAc, Km=3mM) on glycoproteins and glycolipids.
• Critical for leukocyte trafficking in humans (with potentially some role for ST3Gal-6, in mouse), and plays a major role in the synthesis of leukocyte surface sialyl LeX structures.
• ST3Gal-4 also regulates VWF half-life in circulation.
• Widely expressed in human tissues, with high levels in the adrenal gland, placenta, trachea, testis, blood lymphocytes, and colon and significant but lower amounts in the liver, lung, and skeletal muscle.

Related Pathway
KEGG ORTHOLOGY:   
K03494
KEGG Pathway:   
ko00601  ko01100 
Reactome:   
R-HSA-1912420
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q11206 
NCBI GeneID:   
6484
GlyMap GeneID:   
6484
DNA aRefSeq:   
NM_001254757.1 
Protein RefSeq:   
NP_001241686.1 
BRENDA:   
Kinetic info.
OMIM number:   
104240
GeneCards database:   
ST3GAL4
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), O-linked (other), GSL
Compartment:  
     
Substrate:  
     Gal(b1-?)GlcNAc(b1-?) or Gal(b1-3)GalNAc(b1-?)
Product:  
     NeuAc(a2-3)Gal(b1-?)GlcNAc(b1-?) or NeuAc(a2-3)Gal(b1-3)GalNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    7.26e-05    (pmol/cell • hr)       KM:    2.60e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 300 200 100 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-1468-5p 584-591 -0.758 99.0 -0.113 62.0 noncon
hsa-miR-193a-3p 346-353 -0.627 99.0 -0.627 99.0 noncon
hsa-miR-193b-3p 346-353 -0.627 99.0 -0.627 99.0 noncon
hsa-miR-6845-5p 391-398 -0.525 98.0 -0.525 98.0 noncon
hsa-miR-6762-5p 391-398 -0.525 98.0 -0.525 98.0 noncon
hsa-miR-4757-5p 343-350 -0.519 99.0 -0.519 99.0 noncon
hsa-miR-4638-5p 177-184 -0.492 97.0 -0.492 98.0 noncon
hsa-miR-4738-5p 201-208 -0.478 97.0 -0.478 98.0 noncon
hsa-miR-6744-3p 343-350 -0.441 99.0 -0.441 99.0 noncon
hsa-miR-4464 556-563 -0.426 99.0 -0.426 99.0 noncon
hsa-miR-210-5p 435-442 -0.421 98.0 -0.421 98.0 noncon
hsa-miR-3651 286-292 -0.389 96.0 -0.389 98.0 noncon
hsa-miR-4748 556-563 -0.364 99.0 -0.364 99.0 noncon
hsa-miR-4764-5p 331-338 -0.362 97.0 -0.362 97.0 noncon
hsa-miR-6756-3p 527-534 -0.36 96.0 -0.36 97.0 noncon
hsa-miR-3127-3p 527-534 -0.36 96.0 -0.36 97.0 noncon
hsa-miR-216b-5p 272-279 -0.355 98.0 -0.355 99.0 noncon
hsa-miR-4290 429-436 -0.348 93.0 -0.348 95.0 noncon
hsa-miR-4684-5p 351-358 -0.341 98.0 -0.341 99.0 noncon
hsa-miR-5195-5p 300-306 -0.34 97.0 -0.34 98.0 noncon
hsa-miR-6878-3p 344-350 -0.338 96.0 -0.338 97.0 noncon
hsa-miR-3142 401-408 -0.322 98.0 -0.322 98.0 noncon
hsa-miR-92a-2-5p 394-401 -0.313 97.0 -0.313 97.0 noncon
hsa-miR-593-5p 77-84 -0.313 93.0 -0.313 95.0 noncon
hsa-miR-4648 325-332 -0.31 96.0 -0.31 97.0 noncon
hsa-miR-128-1-5p 392-398 -0.306 95.0 -0.306 96.0 noncon
hsa-miR-216a-5p 273-279 -0.302 98.0 -0.302 99.0 noncon
hsa-miR-4749-3p 469-475 -0.3 93.0 -0.3 95.0 noncon
hsa-miR-1245b-5p 401-408 -0.296 97.0 -0.296 98.0 noncon
hsa-miR-3922-3p 345-351 -0.295 97.0 -0.295 98.0 noncon
hsa-miR-8078 405-412 -0.287 95.0 -0.287 96.0 noncon
hsa-miR-3176 345-351 -0.285 97.0 -0.285 97.0 noncon
hsa-miR-6878-3p 149-155 -0.285 94.0 -0.285 96.0 noncon
hsa-miR-2115-5p 588-594 -0.279 94.0 0.0 9.0 noncon
hsa-miR-500b-3p 43-50 -0.277 98.0 -0.277 98.0 noncon
hsa-miR-3150a-5p 549-555 -0.276 98.0 -0.276 99.0 noncon
hsa-miR-3150b-5p 549-555 -0.276 98.0 -0.276 99.0 noncon
hsa-miR-3065-3p 311-317 -0.276 96.0 -0.276 97.0 noncon
hsa-miR-1227-5p 392-398 -0.272 95.0 -0.272 96.0 noncon
hsa-miR-516a-3p 588-594 -0.268 94.0 0.0 15.0 noncon
hsa-miR-516b-3p 588-594 -0.268 94.0 0.0 15.0 noncon
hsa-miR-371a-3p 413-419 -0.268 95.0 -0.268 97.0 noncon
hsa-miR-128-2-5p 392-398 -0.267 96.0 -0.267 97.0 noncon
hsa-miR-651-5p 579-585 -0.264 97.0 -0.264 97.0 noncon
hsa-miR-4529-5p 389-395 -0.258 90.0 -0.258 93.0 noncon