GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
POMGNT1



General Information
Gene Symbol:   
POMGNT1
Protein name:   
Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT13
E.C. No:   
2.4.1.*
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• O-mannosyl glycans are a type of O-glycan in which the reducing terminal mannose is attached to Ser/Thr.
• POMGnT1 catalyzes the transfer of β1,2GlcNAc from UDP-GlcNAc to O-mannose of glycoproteins.
• POMGnT1 deficiency results in muscle-eye-brain disease (MEB), a form of congenital muscular dystrophy/ a-dystroglycanopathy.
• POMGnT1 cannot recognize 6P-Man-peptide as an acceptor. Thus O-mannose phosphorylation occurs after the formation of GlcNAcβ1-2Man linkage.

Related Pathway
KEGG ORTHOLOGY:   
K09666
KEGG Pathway:   
ko00515  ko01100 
Reactome:   
R-HSA-5083628
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q8WZA1 
NCBI GeneID:   
55624
GlyMap GeneID:   
55624
DNA aRefSeq:   
NM_017739.3 
Protein RefSeq:   
NP_060209.3 
BRENDA:   
Kinetic info.
OMIM number:   
606822
GeneCards database:   
POMGNT1
Enzyme Specificity
Glycan Type:  
     O-linked (other)
Compartment:  
     ER
Substrate:  
     Man(a1-?)Sre|Thr
Product:  
     GlcNAc(b1-2)Man(a1-?)Sre|Thr
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
POMGNT1/hsa-miR-4755-3p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...AGCAUCUCUGUGUG ACUAGAU U...
3'   UGAAAGGGAAGUCUC GGACCG A
 6mer 1942-1948 -0.286 94.0 -0.286 96.0 noncon