GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
B3GLCT



General Information
Gene Symbol:   
B3GLCT
Protein name:   
Beta-1,3-glucosyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT31
E.C. No:   
2.4.1.*
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• During O-Fuc modification of TSR1 (thrombospondin type 1) repeat, the first step involves the transfer of fuc by Pofut2 to Ser/Thr in this domain. The absence of Pofut2 in mice results in early embryonic lethality with defects in gastrulation. 
• The next step involves the transfers glucose to O-Fuc by a gene encoding the β3-glucosyltransferase, B3GLCT or B3GALTL. Mutations in B3GLCT in humans results in a multifaceted severe developmental disorder known as Peters-plus syndrome.
• Besides O-fuc, and C-mannosylation also occur on TSR1/thrombospondin-1 domains.

Related Pathway
KEGG ORTHOLOGY:   
K13675
KEGG Pathway:   
ko00514 
Reactome:   
R-HSA-5083635
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q6Y288 
NCBI GeneID:   
145173
GlyMap GeneID:   
145173
DNA aRefSeq:   
NM_194318.3 
Protein RefSeq:   
NP_919299.3 
BRENDA:   
Kinetic info.
OMIM number:   
610308
GeneCards database:   
B3GLCT
Enzyme Specificity
Glycan Type:  
     O-linked (other)
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Fuc(a1-?)Ser|Thr
Product:  
     Glc(b1-3)Fuc(a1-?)Ser|Thr
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 8 6 4 2 0 RPKM
Expression Data in Cells
No expression data available.
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
B3GLCT/hsa-miR-135a-5p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'... CAGCACAGAAUUUA AAGCCAUA ...
3'   AGUGUAUCCUUAUUU UUCGGUA U
 8mer 1239-1246 -0.359 97.0 -0.185 91.0 con
5'... AUAUAAAGUUUAUA AAGCCAU U...
3'   AGUGUAUCCUUAUUU UUCGGUA U
 7mer-m8 2049-2055 -0.187 87.0 -0.086 80.0 noncon
5'... UCAUUUUUGCUUUU AAGCCAU U...
3'   AGUGUAUCCUUAUUU UUCGGUA U
 7mer-m8 2274-2280 -0.166 85.0 -0.076 78.0 noncon