GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
UST



General Information
Gene Symbol:   
UST
Protein name:   
Uronyl 2-sulfotransferase
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.*
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Chondroitin sulfate chains are composed of GlcAβ1-3GalNAcβ1-4 repeating unit. Various sulfotransferases, CHST3, 7, 11, 12, 13, 14, 15, and UST, sulfate either GalNAc and GlcA residues. CS are a major functional component of aggrecan, and needed for cartilage formation and maintenance.
• This is also called CS2ST. It transfers sulfo group to C2 of GlcA or IdoA in the context of GalNAc.
• IdoA2S residue present in a highly sulfated chondroitin sulfate B (dermatan sulfate) hexasaccharide
• Does not have activity on HS

Related Pathway
KEGG ORTHOLOGY:   
K03193
KEGG Pathway:   
ko00532 
Reactome:   
R-HSA-2022923
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9Y2C2 
NCBI GeneID:   
10090
GlyMap GeneID:   
10090
DNA aRefSeq:   
NM_005715.2 
Protein RefSeq:   
NP_005706.1 
BRENDA:   
Kinetic info.
OMIM number:   
610752
GeneCards database:   
UST
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     
Substrate:  
     GalNAc(b1-4)GlcA(b1-3)GalNAc(b1-?) or GalNAc(b1-4)IdoA(a1-3)GalNAc(b1-?)
Product:  
     GalNAc(b1-4)GlcA(b1-3 [2S])GalNAc(b1-?) or GalNAc(b1-4)IdoA(a1-3 [2S])GalNAc(b1-?)
Constraints:  
     var=50%
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000367463 ZNF263 NFATC3 TAF1 ARNT SMAD4 MYC IRF4 RBPJ ZNF554 NFKB2 TEAD4 TP53 NFKB1 UBTF KLF15 SP4 TCF4 TCF4 HSF1 SP2 GATA3 SP3 SP3 ZNF467 ZSCAN22 FEZF1 FEZF1 FEZF1 MZF1 PRDM1 SP1 OSR2 OSR2 YY1 FOXP1 BHLHE40 GATA6 GATA6 GATA6 SP7 DEAF1 SIX5 USF2 USF2 EGR3 IKZF2 RELB RELB ZNF341 EGR2 EGR2 SMAD2 MAZ MAZ VEZF1 NFAT5 PATZ1 ZNF507 NFE2 ZBTB7A EGR1 ZEB2 ZNF629
miRNA-GlycoEnzyme Interactome {ref. 9}
UST/hsa-miR-584-3p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...UCAAAAAUAUAUAU GGAACUGA ...
3'   UCGGACCAACCGGA CCUUGAC U
 8mer 2828-2835 -0.536 99.0 -0.337 98.0 noncon