GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
CHST15



General Information
Gene Symbol:   
CHST15
Protein name:   
Carbohydrate sulfotransferase 15
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.33
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Chondroitin sulfate chains are composed of GlcAβ1-3GalNAcβ1-4 repeating unit. Various sulfotransferases, CHST3, 7, 11, 12, 13, 14, 15, and UST, sulfate either GalNAc and GlcA residues. CS are a major functional component of aggrecan, and needed for cartilage formation and maintenance.
• This enzyme synthesizes GlcA(β1-3)GalNAc(β1-4 [4, 6-bissulfate]) or IdoA(α1-3)GalNAc(β1-4 [4, 6-bissulfate]) repeating units at varying proportion Chondroitin/dermatan sulfate
• This enzyme is widely expressed. Mice deficient in this enzyme are born normal and fertile.
• GalNAc4S-6ST is highly expressed in colorectal cancer cells. It is often increased in cancer tissue.

Related Pathway
KEGG ORTHOLOGY:   
K08106
KEGG Pathway:   
ko00532 
Reactome:   
R-HSA-2022870
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q7LFX5 
NCBI GeneID:   
51363
GlyMap GeneID:   
51363
DNA aRefSeq:   
NM_001270764.1 
Protein RefSeq:   
NP_001257693.1 
BRENDA:   
Kinetic info.
OMIM number:   
608277
GeneCards database:   
CHST15
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GlcA(b1-3)GalNAc(b1-4 [6S]) or IdoA(a1-3)GalNAc(b1-4 [6S])
Product:  
     GlcA(b1-3)GalNAc(b1-4 [4,6S]) or IdoA(a1-3)GalNAc(b1-4 [4,6S])
Constraints:  
     var=50%
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 12 10 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6729-5p 1409-1416 -0.45 94.0 -0.45 96.0 noncon
hsa-miR-4649-5p 1409-1416 -0.442 94.0 -0.442 95.0 noncon
hsa-miR-4799-3p 889-896 -0.439 99.0 -0.439 99.0 noncon
hsa-miR-5588-5p 889-896 -0.423 99.0 -0.423 99.0 noncon
hsa-miR-4720-5p 889-896 -0.418 98.0 -0.418 99.0 noncon
hsa-miR-6794-5p 435-442 -0.399 97.0 -0.399 98.0 noncon
hsa-miR-4716-3p 435-442 -0.388 96.0 -0.388 97.0 noncon
hsa-miR-6780b-3p 1965-1972 -0.387 98.0 -0.376 98.0 noncon
hsa-miR-4700-3p 2361-2368 -0.37 99.0 -0.348 99.0 noncon
hsa-miR-654-5p 1412-1419 -0.351 97.0 -0.351 97.0 noncon
hsa-miR-647 2474-2481 -0.349 98.0 -0.027 75.0 noncon
hsa-miR-541-3p 1412-1419 -0.343 97.0 -0.343 97.0 noncon
hsa-miR-3115 2433-2440 -0.331 94.0 -0.311 96.0 noncon
hsa-miR-6852-5p 102-109 -0.328 98.0 -0.328 98.0 noncon
hsa-miR-483-5p 1439-1446 -0.326 94.0 -0.326 96.0 noncon
hsa-miR-3186-5p 2445-2451 -0.325 95.0 -0.159 90.0 noncon
hsa-miR-4721 947-954 -0.319 95.0 -0.319 96.0 noncon
hsa-miR-3912-3p 770-776 -0.316 93.0 -0.316 96.0 noncon
hsa-miR-3199 2097-2104 -0.314 95.0 -0.295 96.0 noncon
hsa-miR-4663 173-180 -0.31 97.0 -0.31 98.0 noncon
hsa-miR-3657 1553-1560 -0.302 93.0 -0.302 95.0 noncon
hsa-miR-8052 2097-2104 -0.301 95.0 -0.283 95.0 noncon
hsa-miR-615-5p 183-189 -0.29 93.0 -0.29 94.0 noncon
hsa-miR-1288-3p 2096-2102 -0.287 93.0 -0.27 95.0 noncon
hsa-miR-1236-3p 867-874 -0.286 96.0 -0.286 96.0 noncon
hsa-miR-6738-5p 185-192 -0.278 95.0 -0.278 96.0 noncon
hsa-miR-6741-3p 1194-1200 -0.277 93.0 -0.277 95.0 noncon
hsa-miR-2467-3p 2469-2476 -0.266 95.0 -0.131 85.0 noncon
hsa-miR-4433b-3p 1984-1991 -0.251 95.0 -0.244 96.0 noncon