GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
CHST14



General Information
Gene Symbol:   
CHST14
Protein name:   
Carbohydrate sulfotransferase 14
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.35
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Chondroitin sulfate chains are composed of GlcAβ1-3GalNAcβ1-4 repeating unit. Various sulfotransferases, CHST3, 7, 11, 12, 13, 14, 15, and UST, sulfate either GalNAc and GlcA residues. CS are a major functional component of aggrecan, and needed for cartilage formation and maintenance.
• Dermatan 4-O sulfotransferase 1 (D4ST1) is the CS/DS 4-O-sulfotransferase responsible for the sulfation of GalNAc in blocks of IdoA-GalNAc-IdoA.
• It acts both nearly fully or partially desulfated DS, but not on CS.
• Chst14/D4st1-deficient mice showed growth retardation as well as multiple system abnormalities including neurological changes.

Related Pathway
KEGG ORTHOLOGY:   
K08105
KEGG Pathway:   
ko00532 
Reactome:   
R-HSA-2022923
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q8NCH0 
NCBI GeneID:   
113189
GlyMap GeneID:   
113189
DNA aRefSeq:   
NM_130468.3 
Protein RefSeq:   
NP_569735.1 
BRENDA:   
Kinetic info.
OMIM number:   
608429
GeneCards database:   
CHST14
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     IdoA(a1-3)GalNAc(b1-4)IdoA(a1-3) or IdoA(a1-3 [2S])GalNAc(b1-4)IdoA(a1-3) or IdoA(a1-3)GalNAc(b1-4)IdoA(a1-3 [2S]) or IdoA(a1-3 [2S])GalNAc(b1-4)IdoA(a1-3 [2S])
Product:  
     IdoA(a1-3)GalNAc(b1-4 [4S])IdoA(a1-3) or IdoA(a1-3 [2S])GalNAc(b1-4 [4S])IdoA(a1-3) or IdoA(a1-3)GalNAc(b1-4 [4S])IdoA(a1-3 [2S]) or IdoA(a1-3 [2S])GalNAc(b1-4 [4S])IdoA(a1-3 [2S])
Constraints:  
     var=50%
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
No expression data available.
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4711-5p 716-723 -0.689 99.0 -0.686 99.0 noncon
hsa-miR-608 417-424 -0.582 98.0 -0.582 99.0 noncon
hsa-miR-1468-5p 540-547 -0.561 95.0 -0.559 96.0 noncon
hsa-miR-4651 417-424 -0.561 98.0 -0.561 98.0 noncon
hsa-miR-1288-3p 305-312 -0.524 99.0 -0.524 99.0 noncon
hsa-miR-4736 505-512 -0.509 99.0 -0.507 99.0 noncon
hsa-miR-6081 480-487 -0.498 99.0 -0.496 99.0 noncon
hsa-miR-1911-3p 559-566 -0.496 99.0 -0.494 99.0 noncon
hsa-miR-5010-5p 404-411 -0.483 98.0 -0.483 99.0 noncon
hsa-miR-8065 498-505 -0.479 98.0 -0.477 98.0 noncon
hsa-miR-6773-5p 2216-2222 -0.474 98.0 -0.002 30.0 noncon
hsa-miR-588 336-343 -0.474 99.0 -0.474 99.0 noncon
hsa-miR-151a-5p 864-871 -0.469 96.0 -0.002 11.0 noncon
hsa-miR-151b 864-871 -0.469 96.0 -0.002 11.0 noncon
hsa-miR-383-5p.2 262-269 -0.468 99.0 -0.468 99.0 noncon
hsa-miR-296-5p 1821-1828 -0.463 97.0 -0.002 21.0 noncon
hsa-miR-1178-3p 442-449 -0.459 99.0 -0.459 99.0 noncon
hsa-miR-4763-5p 1553-1560 -0.454 97.0 -0.002 25.0 noncon
hsa-miR-4701-5p 336-343 -0.442 99.0 -0.442 99.0 noncon
hsa-miR-6796-5p 661-668 -0.439 98.0 -0.437 98.0 noncon
hsa-miR-6724-5p 2216-2222 -0.438 98.0 -0.002 32.0 noncon
hsa-miR-554 434-441 -0.435 98.0 -0.435 98.0 noncon
hsa-miR-6779-3p 179-186 -0.431 99.0 -0.431 99.0 noncon
hsa-miR-6784-5p 1479-1486 -0.426 96.0 -0.002 21.0 noncon
hsa-miR-3162-5p 640-647 -0.418 97.0 -0.416 98.0 noncon
hsa-miR-6838-3p 432-439 -0.412 99.0 -0.412 99.0 noncon
hsa-miR-4739 639-646 -0.408 99.0 -0.406 99.0 noncon
hsa-miR-1321 639-646 -0.403 99.0 -0.401 99.0 noncon
hsa-miR-4756-5p 639-646 -0.403 99.0 -0.401 99.0 noncon
hsa-miR-491-5p 660-667 -0.402 98.0 -0.4 98.0 noncon
hsa-miR-210-5p 363-370 -0.4 98.0 -0.4 98.0 noncon
hsa-miR-6842-5p 1767-1774 -0.399 92.0 -0.002 13.0 noncon
hsa-miR-544b 429-436 -0.391 99.0 -0.391 99.0 noncon
hsa-miR-7110-5p 1767-1774 -0.383 90.0 -0.002 13.0 noncon
hsa-miR-3616-3p 128-135 -0.376 96.0 -0.376 97.0 noncon
hsa-miR-4532 285-291 -0.369 95.0 -0.369 96.0 noncon
hsa-miR-505-5p 275-282 -0.365 98.0 -0.365 98.0 noncon
hsa-miR-1915-5p 1557-1564 -0.363 96.0 -0.002 30.0 noncon
hsa-miR-637 1157-1164 -0.363 97.0 -0.002 30.0 noncon
hsa-miR-124-3p.1 472-478 -0.362 97.0 -0.361 98.0 con
hsa-miR-3672 487-494 -0.362 98.0 -0.361 99.0 noncon
hsa-miR-4525 404-411 -0.357 98.0 -0.357 98.0 noncon
hsa-miR-6724-5p 1708-1715 -0.356 97.0 -0.002 31.0 noncon
hsa-miR-6852-3p 173-180 -0.349 96.0 -0.349 97.0 noncon
hsa-miR-3909 173-180 -0.349 96.0 -0.349 97.0 noncon
hsa-miR-5581-5p 1549-1556 -0.346 95.0 -0.002 28.0 noncon
hsa-miR-296-5p 2216-2222 -0.344 93.0 -0.002 20.0 noncon
hsa-miR-4297 1549-1556 -0.344 95.0 -0.002 29.0 noncon
hsa-miR-379-5p 317-323 -0.342 97.0 -0.342 98.0 noncon
hsa-miR-6851-3p 2214-2220 -0.337 96.0 -0.002 23.0 noncon
hsa-miR-6812-5p 418-424 -0.337 95.0 -0.337 96.0 noncon
hsa-miR-4653-5p 345-352 -0.335 98.0 -0.335 98.0 noncon
hsa-miR-6773-5p 1708-1715 -0.335 96.0 -0.002 28.0 noncon
hsa-miR-4710 2095-2102 -0.334 97.0 -0.002 34.0 noncon
hsa-miR-6752-5p 1767-1774 -0.331 91.0 -0.002 17.0 noncon
hsa-miR-3943 975-981 -0.329 96.0 -0.002 30.0 noncon
hsa-miR-5582-5p 471-477 -0.326 97.0 -0.325 98.0 noncon
hsa-miR-873-3p 486-493 -0.325 98.0 -0.324 99.0 noncon
hsa-miR-760 379-386 -0.324 95.0 -0.324 96.0 noncon
hsa-miR-4446-3p 2085-2092 -0.324 97.0 -0.002 29.0 noncon
hsa-miR-6508-3p 1654-1661 -0.323 97.0 -0.002 23.0 noncon
hsa-miR-7150 506-512 -0.321 97.0 -0.32 97.0 noncon
hsa-miR-3921 345-352 -0.319 98.0 -0.319 98.0 noncon
hsa-miR-548a-3p 800-807 -0.319 99.0 -0.318 99.0 noncon
hsa-miR-6868-5p 507-513 -0.317 97.0 -0.316 98.0 noncon
hsa-miR-4725-3p 658-665 -0.316 95.0 -0.315 96.0 noncon
hsa-miR-6780b-5p 658-665 -0.316 95.0 -0.315 96.0 noncon
hsa-miR-4470 240-247 -0.315 98.0 -0.315 98.0 noncon
hsa-miR-548ar-3p 800-807 -0.314 99.0 -0.313 99.0 noncon
hsa-miR-4313 1935-1941 -0.309 93.0 -0.001 15.0 noncon
hsa-miR-548f-3p 800-807 -0.308 99.0 -0.307 99.0 noncon
hsa-miR-6778-3p 1834-1841 -0.307 96.0 -0.001 34.0 noncon
hsa-miR-548av-3p 799-805 -0.307 99.0 -0.306 99.0 noncon
hsa-miR-3943 1936-1942 -0.303 95.0 -0.001 30.0 noncon
hsa-miR-6752-3p 1838-1845 -0.3 94.0 -0.001 32.0 noncon
hsa-miR-363-5p 416-422 -0.299 95.0 -0.299 96.0 noncon
hsa-miR-3529-5p 317-323 -0.299 95.0 -0.299 97.0 noncon
hsa-miR-4665-5p 405-411 -0.297 93.0 -0.297 95.0 noncon
hsa-miR-6747-3p 186-193 -0.296 96.0 -0.296 97.0 noncon
hsa-miR-371b-3p 1948-1954 -0.296 94.0 -0.001 27.0 noncon
hsa-miR-3191-5p 1600-1607 -0.293 97.0 -0.001 40.0 noncon
hsa-miR-548e-3p 800-807 -0.292 99.0 -0.291 99.0 noncon
hsa-miR-6745 416-422 -0.291 95.0 -0.291 96.0 noncon
hsa-miR-3665 504-510 -0.29 94.0 -0.289 96.0 noncon
hsa-miR-4324 103-110 -0.288 97.0 -0.288 98.0 noncon
hsa-miR-1178-3p 1517-1524 -0.285 96.0 -0.001 39.0 noncon
hsa-miR-548az-3p 800-807 -0.284 99.0 -0.283 99.0 noncon
hsa-miR-383-5p.1 261-267 -0.282 93.0 -0.282 95.0 noncon
hsa-miR-129-1-3p 761-767 -0.281 96.0 -0.28 97.0 noncon
hsa-miR-129-2-3p 761-767 -0.281 96.0 -0.28 97.0 noncon
hsa-miR-6737-5p 418-424 -0.281 93.0 -0.281 95.0 noncon
hsa-miR-6819-5p 418-424 -0.281 93.0 -0.281 94.0 noncon
hsa-miR-6894-3p 1554-1561 -0.281 96.0 -0.001 47.0 noncon
hsa-miR-1199-5p 2021-2028 -0.28 96.0 -0.001 42.0 noncon
hsa-miR-6751-3p 2021-2028 -0.277 95.0 -0.001 37.0 noncon
hsa-miR-4633-3p 565-572 -0.274 96.0 -0.273 97.0 noncon
hsa-miR-324-3p 227-234 -0.274 95.0 -0.274 96.0 noncon
hsa-miR-3144-3p 920-927 -0.272 94.0 -0.001 22.0 noncon
hsa-miR-4271 658-665 -0.272 95.0 -0.271 96.0 noncon
hsa-miR-548k 738-744 -0.271 98.0 -0.27 99.0 noncon
hsa-miR-3202 652-658 -0.271 95.0 -0.27 96.0 noncon
hsa-miR-6749-3p 2185-2191 -0.27 92.0 -0.001 32.0 noncon
hsa-miR-3176 1728-1735 -0.269 96.0 -0.001 33.0 noncon
hsa-miR-3165 415-421 -0.268 96.0 -0.268 97.0 noncon
hsa-miR-6741-5p 589-596 -0.266 97.0 -0.265 98.0 noncon
hsa-miR-5001-5p 2084-2090 -0.264 90.0 -0.001 21.0 noncon
hsa-miR-6760-5p 649-655 -0.264 96.0 -0.263 97.0 noncon
hsa-miR-1913 227-234 -0.264 94.0 -0.264 96.0 noncon
hsa-miR-6773-5p 1822-1829 -0.261 91.0 -0.001 28.0 noncon
hsa-miR-548av-5p 738-744 -0.26 98.0 -0.259 99.0 noncon
hsa-miR-1976 188-195 -0.259 96.0 -0.259 97.0 noncon
hsa-miR-4531 2129-2136 -0.257 96.0 -0.001 22.0 noncon
hsa-miR-3934-5p 503-509 -0.254 97.0 -0.253 98.0 noncon
hsa-miR-6864-3p 487-494 -0.252 98.0 -0.251 99.0 noncon
hsa-miR-4514 60-66 -0.252 96.0 -0.252 97.0 noncon
hsa-miR-3922-3p 1728-1735 -0.251 96.0 -0.001 33.0 noncon
hsa-miR-548ba 453-459 -0.251 96.0 -0.251 97.0 noncon
hsa-miR-2467-5p 2118-2124 -0.251 95.0 -0.001 48.0 noncon
hsa-miR-365b-5p 2079-2086 -0.25 94.0 -0.001 23.0 noncon
hsa-miR-6724-5p 1822-1829 -0.25 91.0 -0.001 30.0 noncon
hsa-miR-576-3p 257-263 -0.25 95.0 -0.25 97.0 noncon
hsa-miR-365a-5p 2079-2086 -0.25 94.0 -0.001 23.0 noncon