Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (firstname.lastname@example.org), Yusen Zhou (email@example.com) or Ted Groth (firstname.lastname@example.org).
• Sulfation of heparan sulfate occurs at C2 of HexA. It also occurs at C3, C6, and NS residues of GlcNAc or GlcNS. HSPGs are major regulators of cell behavior with roles in inflammation, blood coagulation, tumor invasion, and malignancy.
• Three heparan sulfate 6-O-sulfotransferases exist that transfer sulfate to the C6 position of GlcNAc or GlcNS in heparan sulfate/heparin.
• HS6ST1 prefers iduronic acid residues adjacent to the GlcNS. HS6ST2 prefers GlcA when substrate conc is high, but it prefers IdoA at low substrate concentration. HS6ST-3 preference is intermediate between HS6ST1 and HS6ST2. These enzymes do not function when IdoA is 2-sulfated.
• HS6ST1 is ubiquitously expressed. The expression of other isoenzymes is restricted. HS6ST2 is expressed primarily in bladder, brain, testis, and thyroid. HS6ST3 slightly in brain, connective tissue, and kidney.
• HS6ST1 deficient mice display growth retardation and fatality after E15.5. H6ST1 is also associated with human disease.
• These enzymes do not act on CS/DS/KS.