GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
HS3ST3A1



General Information
Gene Symbol:   
HS3ST3A1
Protein name:   
Heparan sulfate glucosamine 3-O-sulfotransferase 3A1
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.30
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Sulfation of heparan sulfate occurs at C2 of HexA. It also occurs at C3, C6, and NS residues of GlcNAc or GlcNS. HSPGs are major regulators of cell behavior with roles in inflammation, blood coagulation, tumor invasion, and malignancy.
• Seven isoenzymes display HS 3-O-sulfotransferase activity, with all enzymes transferring sulfate to C3 of GlcNAc in heparin and heparan sulfate.
• HS3ST1 sulfates an N-sulfo glucosamine (GlcNS) that has a nonsulfated glucuronic acid (GlcA) or an iduronic acid (IdoA) at the nonreducing end. This modification is necessary for anti-thrombin activity of heparin. HS
• HS3ST-2, 3 (3A and 3B), 4, and 6, all sulfate a GlcNS6S residue that has a 2-O-sulfated iduronic acid (IdoA2S) on the nonreducing end. This does not provide anti-thrombin activity, but serves viral entry for herpes simplex virus type-1.
• The tissue distribution of the different HS3STs is quite different.

Related Pathway
KEGG ORTHOLOGY:   
K07809
KEGG Pathway:   
ko00534 
Reactome:   
R-HSA-2022928
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9Y663 
NCBI GeneID:   
9955
GlyMap GeneID:   
9955
DNA aRefSeq:   
NM_006042.2 
Protein RefSeq:   
NP_006033.1 
BRENDA:   
Kinetic info.
OMIM number:   
604057
GeneCards database:   
HS3ST3A1
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     IdoA(a1-4 [2S])GlcN(a1-4 [2S]) or IdoA(a1-4 [2S])GlcN(a1-4 [2,6S])
Product:  
     IdoA(a1-4 [2S])GlcN(a1-4 [2,3S]) or IdoA(a1-4 [2S])GlcN(a1-4 [2,3,6S])
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 50 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000284110 ATF2 FLI1 EGR3 RUNX1 ESR1 GLIS1 YY1 NR2F2 SP1 FLI1 ZBTB26 FOXP1 KLF9 EGR3 ZBTB42 ZNF76 ZNF341 ZNF341 ZNF35 ZNF600 ZNF600 ZNF317 ZNF549 IKZF1 KLF9 MAX SP2 SP2 SP2 ZNF263 GATA3 ZBTB17 ZBTB6 ZNF554 ZNF467 PRDM10 RUNX1 ZFP64 ZBTB20 GATA1 MYCN ZNF335 GLIS2 ZEB2 PBX2 PKNOX1 PBX3 GABPA MYC ZNF778 KLF8 SMAD1 SP2 FOXH1 SP4 ZBTB7A ZBTB7A ZNF223 YY1 KLF1 SP1 KLF15 KLF15 ZNF449 ZNF449 SP3 SP3 SP2 NEUROD1 PATZ1 ZNF320 NANOG SMAD3 SMAD4 EGR1 PAX5 ETV1 KLF12 VEZF1 ZBTB26 MTA1 MAZ ATF7 ZNF629 ATF2 JUND KMT2A CREM CREM E2F1 E2F1 ATF3 ZNF574 ZNF574 ATF1 TBP ZFP64 BHLHE40 BHLHE40 ZSCAN22 MAX USF2 CTCFL GLIS1 FOXM1 MNT MNT MBD1 MBD1 MXI1 NONO
miRNA-GlycoEnzyme Interactome {ref. 9}
HS3ST3A1/hsa-miR-7111-3p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...AUUAAAUUGGUUUA GAGAGGAA ...
3'   GACCCUCCUCCCUU CUCUCCU A
 8mer 1489-1496 -0.276 96.0 -0.137 89.0 noncon
5'...GCUUGAUUGAAUUG GAGAGGAA ...
3'   GACCCUCCUCCCUU CUCUCCU A
 8mer 932-939 -0.234 94.0 -0.131 88.0 noncon
5'...GAAAUUUAAACCCA AGAGGAA A...
3'   GACCCUCCUCCCUUC UCUCCU A
 7mer-A1 1569-1575 -0.011 43.0 -0.006 46.0 noncon