GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
GALNS



General Information
Gene Symbol:   
GALNS
Protein name:   
N-acetylgalactosamine-6-sulfatase
Enzyme Class:   
CAZy Family:   
E.C. No:   
3.1.6.4
Organs:   
Reaction (SNFG):   
→
→
Reaction (IUBMB):   
No specified reaction.
About

• Hydrolyzes the 6 carbon sulfates on GalNAc residues on chondroitin sulfate and the 6 carbon sulfates on Gal residues on keratin sulfate.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-2022857
Related Database
Organism:   
Homo sapiens
Uniprot:   
P34059 
NCBI GeneID:   
2588
GlyMap GeneID:   
2588
DNA aRefSeq:   
NM_000512.4 
Protein RefSeq:   
NP_000503.1 
BRENDA:   
Kinetic info.
OMIM number:   
612222
GeneCards database:   
GALNS
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     
Substrate:  
     GalNAc(b1-4 S-6)GlcA(b1-3) or Gal(b1-4 S-6)GlcNAc(b1-3)
Product:  
     GalNAc(b1-4)GlcA(b1-3) or Gal(b1-4)GlcNAc(b1-3)
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 60 50 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000268695 ENST00000562593 ENST00000565364 ENST00000568311 SOX2 CTCF MYB USF1 SP1 ZFHX2 TAL1 EGR2 EGR1 EGR1 CREM ATF2 JUND MAX BHLHE40 USF2 CEBPB USF1 FOSL1 MITF FOS ZNF770
miRNA-GlycoEnzyme Interactome {ref. 9}
GALNS/hsa-miR-4649-5p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'... GCUUGGCCCUCCUC CUCGCCCA ...
3'   GAGACUCUCGGGUGGG GAGCGGG U
 8mer 189-196 -0.524 97.0 -0.524 97.0 noncon
5'... UCAGGACCCUCCUC CUCGCCCA ...
3'   GAGACUCUCGGGUGGG GAGCGGG U
 8mer 225-232 -0.501 96.0 -0.501 97.0 noncon