Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).
Glycogene class |
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Pathway figs |
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Glycosylation |
Sugar Nucleotide |
Glucuronidation |
Glycan Degradation |
Pathway Map |
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PAPS | + |
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→ |
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+ | PAP |
• Chondroitin sulfate chains are composed of GlcAβ1-3GalNAcβ1-4 repeating unit. Various sulfotransferases, CHST3, 7, 11-13, 14, 15, and UST, sulfate either GalNAc and GlcA residues. CS are a major functional component of aggrecan, and needed for cartilage formation and maintenance.
• CHST11-13 are three isoforms of the C4ST family, C4ST-1, C4ST-2 and C4ST-3.
• These enzymes transfer sulfate from PAPS donor to C4 of GalNAc on CS chains.
• CHST11 transfer sulfate to GalNAc residue adjacent to the reducing side of the GlcA, and also at the non-reducing end. Chondroitin 4-sulfate formed by C4STs serve as acceptors for GalNAc4S-6ST to form
chondroitin sulfate E.
• CHST12 acts on GalNAc adjacent not only to GlcA but also IdoA in desulfated dermatan sulfate. The activity for the latter is lower.
• CHST13 displays similar activity to CHST11
ER | Cis- | Medial- | Trans- | TGN |
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CHST11/hsa-miR-1914-3p |
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miRNA Target | Site Type | Pos. | Context Score | Frac. | Wt. Context | Frac. | Conserved | Quality |
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5'...UUGCAAUGGUUUUU
ACCCCUCA
...
3' GGAGGGUCACGCCC UGGGGAG G |
8mer | 1334-1341 | -0.264 | 97.0 | -0.221 | 96.0 | noncon |