GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
CHST8



General Information
Gene Symbol:   
CHST8
Protein name:   
Carbohydrate sulfotransferase 8
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.*
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Catalyzes C4 sulfation of GalNAc in GalNAc(β1-4)GlcNAcβ on N- and O-glycans.
• Required for biosynthesis of glycoprotein hormones lutropin and thyrotropin

Related Pathway
KEGG ORTHOLOGY:   
K09672
KEGG Pathway:   
ko00513  ko01100 
Reactome:   
R-HSA-975578
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9H2A9 
NCBI GeneID:   
64377
GlyMap GeneID:   
64377
DNA aRefSeq:   
NM_001127895.1 
Protein RefSeq:   
NP_001121367.1 
BRENDA:   
Kinetic info.
OMIM number:   
610190
GeneCards database:   
CHST8
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type)
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GalNAc(b1-4)GlcNAc(b1-?)
Product:  
     GalNAc(b1-4 [4S])GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000262622 ENST00000438847 EGR2 EGR2 ZNF549 NFE2 ZBTB17 MYOD1 ZSCAN31 ZNF48 ZFP64 UBTF GRHL2 GRHL2 TFAP4 RARA ARID4B MAZ MAZ ZNF554 MNT SMARCE1 TFE3 TFE3 TFAP2C ZNF644 ZNF644 THAP11 GLIS1 POU5F1 POU5F1 KLF16 KLF16 KLF16 BCL6 KLF6 KLF6 KLF6 MIER2 MIER2 KAT8 RXRB RXRB YY1 YY1 MAX ELF3 HMG20A TCF12 ZNF263 ZBTB14 ZBTB14 THRB
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-133a-3p.2 354-361 -0.503 98.0 -0.503 98.0 noncon
hsa-miR-133b 354-361 -0.503 98.0 -0.503 98.0 noncon
hsa-miR-346 241-248 -0.46 98.0 -0.46 98.0 noncon
hsa-miR-218-5p 426-432 -0.453 99.0 -0.453 99.0 con
hsa-miR-296-5p 307-314 -0.448 96.0 -0.448 97.0 noncon
hsa-miR-875-3p 436-443 -0.336 99.0 -0.048 75.0 noncon
hsa-miR-615-5p 177-184 -0.303 93.0 -0.303 95.0 noncon
hsa-miR-6778-3p 127-134 -0.298 96.0 -0.298 97.0 noncon
hsa-miR-6805-3p 440-446 -0.291 97.0 -0.042 72.0 noncon
hsa-miR-5691 440-446 -0.291 96.0 -0.042 70.0 noncon
hsa-miR-518c-5p 438-444 -0.287 97.0 -0.042 66.0 noncon
hsa-miR-4469 221-228 -0.283 97.0 -0.283 98.0 noncon
hsa-miR-1299 437-443 -0.256 95.0 -0.038 62.0 noncon