GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Glycogene class
A4GNT
ABO
ALG1
ALG10
ALG10B
ALG11
ALG12
ALG13
ALG14
ALG1L
ALG1L2
ALG2
ALG3
ALG5
ALG6
ALG8
ALG9
B3GALNT2
B3GALT1
B3GALT2
B3GALT5
B3GAT1
B3GAT2
B3GNT2
B3GNT3
B3GNT4
B3GNT6
B3GNT7
B3GNT8
B4GALNT2
B4GALNT3
B4GALNT4
B4GALT1
B4GALT2
B4GALT3
B4GALT4
B4GALT5
C1GALT1
C1GALT1C1
CHST1
CHST10
CHST2
CHST3
CHST4
CHST5
CHST8
CHST9
CMAS
CTBS
DAD1
DDOST
DPAGT1
DPM1
DPM2
DPM3
EDEM1
EDEM2
EDEM3
ENGASE
FPGT
FUCA1
FUT1
FUT10
FUT11
FUT2
FUT3
FUT4
FUT5
FUT6
FUT7
FUT8
FUT9
GAL3ST2
GAL3ST3
GAL3ST4
GALNT1
GALNT10
GALNT11
GALNT12
GALNT13
GALNT14
GALNT15
GALNT16
GALNT17
GALNT18
GALNT2
GALNT3
GALNT4
GALNT5
GALNT6
GALNT7
GALNT8
GALNT9
GALNTL6
GANAB
GCNT1
GCNT2
GCNT3
GCNT4
GNPNAT1
GNPTAB
GNPTG
MAN1A1
MAN1A2
MAN1B1
MAN1C1
MAN2A1
MAN2A2
MAN2B1
MAN2B2
MAN2C1
MANBA
MANEA
MGAT1
MGAT2
MGAT3
MGAT4A
MGAT4B
MGAT4C
MGAT4D
MGAT5
MOGS
NAGK
NAGPA
NEU1
NEU2
NEU3
NEU4
PRKCSH
RPN1
RPN2
ST3GAL1
ST3GAL2
ST3GAL3
ST3GAL4
ST3GAL6
ST6GAL1
ST6GAL2
ST6GALNAC1
ST6GALNAC2
ST6GALNAC3
ST6GALNAC4
ST8SIA2
ST8SIA3
ST8SIA4
ST8SIA6
STT3A
STT3B
UGGT1
UGGT2
Pathway Map
CHST8



General Information
Gene Symbol:   
CHST8
Protein name:   
Carbohydrate sulfotransferase 8
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.*
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Catalyzes C4 sulfation of GalNAc in GalNAc(β1-4)GlcNAcβ on N- and O-glycans.
• Required for biosynthesis of glycoprotein hormones lutropin and thyrotropin

Related Pathway
KEGG ORTHOLOGY:   
K09672
KEGG Pathway:   
ko00513  ko01100 
Reactome:   
R-HSA-975578
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9H2A9 
NCBI GeneID:   
64377
GlyMap GeneID:   
64377
DNA aRefSeq:   
NM_001127895.1 
Protein RefSeq:   
NP_001121367.1 
BRENDA:   
Kinetic info.
OMIM number:   
610190
GeneCards database:   
CHST8
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type)
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GalNAc(b1-4)GlcNAc(b1-?)
Product:  
     GalNAc(b1-4 [4S])GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000262622 ENST00000438847 EGR2 EGR2 ZNF549 NFE2 ZBTB17 MYOD1 ZSCAN31 ZNF48 ZFP64 UBTF GRHL2 GRHL2 TFAP4 RARA ARID4B MAZ MAZ ZNF554 MNT SMARCE1 TFE3 TFE3 TFAP2C ZNF644 ZNF644 THAP11 GLIS1 POU5F1 POU5F1 KLF16 KLF16 KLF16 BCL6 KLF6 KLF6 KLF6 MIER2 MIER2 KAT8 RXRB RXRB YY1 YY1 MAX ELF3 HMG20A TCF12 ZNF263 ZBTB14 ZBTB14 THRB
miRNA-GlycoEnzyme Interactome {ref. 9}
CHST8/hsa-miR-875-3p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...AUAAAGCACAUGG UUUCCAGA ...
3'   GUGUUGGAGUCAC AAAGGUC C
 8mer 436-443 -0.336 99.0 -0.048 75.0 noncon