EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 

Pathway Map

CHST7



General Information
Gene Symbol:   
CHST7
Protein name:   
Carbohydrate sulfotransferase 7
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
Organs:   
Reaction (SNFG):   
PAPS +
+ PAP
Reaction (IUBMB):   
No specified reaction.

About

• Five GlcNAc6ST enzymes have been found in humans.
• GlcNAc6ST-1 (CHST2) catalyzes the formation of 6-sulfo sialyl Lewis-X (6-sulfo sLeX, the major L-selectin ligand on HEV. This is also a major enzyme facilitating C6-GlcNAc sulfation of KS. CHST2 acts at the non-reducing end of GlcNAc(β1-3)Gal(β1-4)GlcNAc(β1-3).
• GlcNAc6ST-2 (CHST4) is expressed on High Endothelial Cells of the lymph node (HEV). Both CHST2 and CHST4 can make 6-sulfo sialyl Lewis-X. It is also involved in the biosynthesis of MECA-79 epitope. CHST4 acts internally on LacNAc substrate.
• GlcNAc6ST-3 (CHST5) is a GlcNAc6ST that is functional in the intestines. It is highly expressed in colon and small intestine. CHST5 does not act to form KS, but it may attach sulfate to C6 on O-glycan core-2 glycan at the non-reducing end. As it is located in the intestines, it does not participate in the formation of the sulfated sLeX epitope.
• GlcNAc6ST-4/GST-5 (CHST7) is widely expressed in many organs. Less is known about this enzyme. It may contribute to C6 sulfation of GalNAc during chondroitin sulfate biosynthesis.
• GlcNAc6ST-5 (CHST6) is expressed in the cornea, and involved in the sulfation of keratin sulfate GAGs of the eye. The absence of this enzyme results in macular corneal dystrophy.


Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
Reactome:   

Related Database
Organism:   
Homo sapiens
Uniprot:   
NCBI GeneID:   
GlyMap GeneID:   
DNA aRefSeq:   
Protein RefSeq:   
BRENDA:   
OMIM number:   
GeneCards database:   

Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GlcA(b1-3)GalNAc(b1-4)GlcA(b1-3)
Product:  
     GlcA(b1-3)GalNAc(b1-4 [6S])GlcA(b1-3)
Constraints:  
     var=50%

Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN











Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 50 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000276055 NFKB1 SMAD2 ZSCAN22 KLF5 SP4 ATF1 EBF1 KLF15 SP2 YY1 MAX EGR2 SP3 SP3 SP3 KLF12 EGR1 EGR1 KLF9 MAZ RUNX1 KLF6