EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ABO



General Information
Gene Symbol:   
ABO
Protein name:   
Histo-blood group ABO system transferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT6
E.C. No:   
2.4.1.40
Organs:   
Reaction (SNFG):   
+
→
+ UDP
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• GTA provides α(1,3)GalNAc-transferase activity in blood gp. A individuals.
• GTB provides α(1,3)Gal-transferase activity in blood gp. B individuals.
• Enzyme activity is truncated in O-blood group individuals.
• Four critical differences in amino acid substitution in GTA and GTBenzymes are 176 Arg for A vs. 176 Gly for B, 235 Gly (A) vs. Ser (B), 266 Leu (A) vs. Met (B), and 268 Gly (A) vs. Ala (B). The most common O allele, O1, is characterized by the deletion of nucleotide 261 G, causing a frame shift resulting in a termination codon at 352-4. The transcribed protein is shorter and dysfunctional.

Related Pathway
KEGG ORTHOLOGY:   
K00709
KEGG Pathway:   
ko00601  ko01100 
Reactome:   
R-HSA-9033807
Related Database
Organism:   
Homo sapiens
Uniprot:   
P16442 
NCBI GeneID:   
28
GlyMap GeneID:   
28
DNA aRefSeq:   
NM_020469.2 
Protein RefSeq:   
NP_065202.2 
BRENDA:   
Kinetic info.
OMIM number:   
110300
GeneCards database:   
ABO
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Fuc(a1-2)Gal(b1-?)
Product:  
     Fuc(a1-2)[GalNAc(a1-3)]Gal(b1-?) or Fuc(a1-2)[Gal(a1-3)]Gal(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 10 8 6 4 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data