EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
B3GALNT2



General Information
Gene Symbol:   
B3GALNT2
Protein name:   
UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 2
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT31
E.C. No:   
2.4.1.313
Organs:   
Reaction (SNFG):   
+
→
+ UDP
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• ER resident enzyme.
• Adds GalNAc to create Type I LacdiNAc structures on both N-glycans and O-glycans, but not glycolipid substrates.
• In particular it forms O-Mannosylated structures on α-dystroglycans, and this is necessary for laminin binding. Thus, it is a critical enzyme that is required for normal development of muscle and brain.
• Other enzyme substrates are yet unidentified.

Related Pathway
KEGG ORTHOLOGY:   
K09654
KEGG Pathway:   
ko00515  ko01100 
Reactome:   
R-HSA-5173105
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q8NCR0 
NCBI GeneID:   
148789
GlyMap GeneID:   
148789
DNA aRefSeq:   
NM_001277155.2 
Protein RefSeq:   
NP_001264084.1 
BRENDA:   
Kinetic info.
OMIM number:   
610194
GeneCards database:   
B3GALNT2
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), O-linked (other)
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GlcNAc(b1-?) or GlcNAc(b1-4)...Man(a1-?)Ser|Thr
Product:  
     GalNAc(b1-3)GlcNAc(b1-?) or GalNAc(b1-3)GlcNAc(b1-4)...Man(a1-?)Ser|Thr
Constraints:  
     !@GlcNAc(b1-4)...Man(b1-4)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    5.75e-04    (pmol/cell • hr)       KM:    1.50e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 50 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4467 2660-2667 -0.551 98.0 -0.366 95.0 noncon
hsa-miR-548v 190-197 -0.505 99.0 -0.505 99.0 noncon
hsa-miR-1282 38-45 -0.486 97.0 -0.486 98.0 noncon
hsa-miR-4763-5p 33-40 -0.48 97.0 -0.48 98.0 noncon
hsa-miR-7976 29-36 -0.452 99.0 -0.452 99.0 noncon
hsa-miR-645 1277-1284 -0.425 97.0 -0.294 95.0 noncon
hsa-miR-19a-3p 158-165 -0.391 97.0 -0.391 98.0 con
hsa-miR-19b-3p 158-165 -0.391 97.0 -0.391 98.0 con
hsa-miR-4711-5p 280-287 -0.382 98.0 -0.361 98.0 noncon
hsa-miR-6894-3p 34-41 -0.382 98.0 -0.382 99.0 noncon
hsa-miR-505-3p.1 789-796 -0.373 96.0 -0.34 96.0 noncon
hsa-miR-218-1-3p 575-582 -0.373 97.0 -0.353 97.0 noncon
hsa-miR-4477b 170-177 -0.369 98.0 -0.369 98.0 noncon
hsa-miR-449b-3p 289-296 -0.369 98.0 -0.349 99.0 noncon
hsa-miR-3192-3p 1225-1232 -0.36 99.0 -0.253 97.0 noncon
hsa-miR-187-3p 1400-1407 -0.354 90.0 -0.247 86.0 noncon
hsa-miR-4753-5p 2596-2603 -0.352 97.0 -0.24 95.0 noncon
hsa-miR-6770-3p 2661-2667 -0.347 91.0 -0.236 85.0 noncon
hsa-miR-6760-3p 2400-2407 -0.336 98.0 -0.229 96.0 noncon
hsa-miR-503-3p 638-645 -0.331 96.0 -0.302 97.0 noncon
hsa-miR-101-3p.1 187-194 -0.331 97.0 -0.331 98.0 noncon
hsa-miR-6768-3p 2996-3002 -0.33 98.0 -0.112 89.0 noncon
hsa-miR-7703 52-59 -0.325 98.0 -0.325 98.0 noncon
hsa-miR-4425 1295-1302 -0.325 94.0 -0.227 89.0 noncon
hsa-miR-6776-5p 128-135 -0.324 97.0 -0.324 98.0 noncon
hsa-miR-4762-3p 231-238 -0.311 98.0 -0.311 99.0 noncon
hsa-miR-125a-5p 748-754 -0.311 96.0 -0.284 96.0 con
hsa-miR-7702 20-27 -0.304 95.0 -0.304 97.0 noncon
hsa-miR-6732-5p 1341-1347 -0.301 93.0 -0.211 89.0 noncon
hsa-miR-3150a-3p 1008-1015 -0.301 96.0 -0.275 96.0 noncon
hsa-miR-125b-2-3p 2956-2963 -0.3 98.0 -0.205 96.0 noncon
hsa-miR-7114-5p 1475-1482 -0.299 97.0 -0.209 95.0 noncon
hsa-miR-2053 3043-3050 -0.296 99.0 0.0 0.0 noncon
hsa-miR-4753-5p 2995-3001 -0.294 96.0 -0.101 81.0 noncon
hsa-miR-125b-5p 748-754 -0.289 95.0 -0.264 95.0 con
hsa-miR-4522 17-23 -0.287 95.0 -0.287 97.0 noncon
hsa-miR-342-3p 720-726 -0.283 97.0 -0.259 97.0 noncon
hsa-miR-4319 748-754 -0.267 94.0 -0.244 94.0 con
hsa-miR-6728-3p 15-21 -0.267 96.0 -0.267 97.0 noncon
hsa-miR-199a-5p 2552-2559 -0.265 93.0 -0.182 91.0 noncon
hsa-miR-199b-5p 2552-2559 -0.265 93.0 -0.182 91.0 noncon
hsa-miR-141-5p 223-229 -0.262 95.0 -0.262 96.0 noncon
hsa-miR-4715-5p 2628-2635 -0.262 92.0 -0.18 90.0 noncon
hsa-miR-3144-3p 904-911 -0.262 93.0 -0.24 95.0 noncon
hsa-miR-6763-5p 1008-1015 -0.261 93.0 -0.239 93.0 noncon
hsa-miR-433-3p 1181-1188 -0.254 96.0 -0.233 97.0 noncon
hsa-miR-6719-3p 524-531 -0.253 96.0 -0.24 97.0 noncon
hsa-miR-4266 821-827 -0.253 90.0 -0.232 92.0 noncon
hsa-miR-1261 2949-2956 -0.252 94.0 -0.173 90.0 noncon